EMD Millipore
Anti-phospho-Histone H3 (Ser10)
06-570
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Browse Similar Products: H3 Flow Cytometry Antibodies
Analysis of mammalian cells in the mitotic phase using flow cytometry is easy and sensitive. The phosphorylated histone 3 at the Ser10 is a specific mitosis marker. This antibody, with great specificity and sensitivity to the H3 pSer10, is ideal for the analysis of mitotic cells.
Flow Cytometry
U2OS osteosarcoma cells
1:500 in PBS with 1% BSA for 2hr at room temperature
1% BSA
Anti-rabbit-Alex Fluor 488
NA
BD LSR II
U2OS cells treated with nocodazole for the G2/M arrest were incubated with this antibody. The blue dots in the box depicted the mitotic cells.
None
Specificity and sensitivity
Not Fluor conjugated. Need a fluor-conjugated secondary antibody
Overall this is a very good antibody for detecting the mitotic cells.