Santa Cruz Biotechnology, Inc.
Anti-tubulin
sc-5286
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Browse Similar Products: Tubulin Western blot Antibodies
Our research lab is focused on understanding the role of oncogenes, such as EGFR, in the metabolism, signaling pathways, growth and metastasis of human cancers including prostate, breast, colon and lung. This antibody was used to detect tubulin in PC3 cells through western blot analysis. This antibody was purchased from Santa Cruz and detected band for tubulin in western blot.
Western Blot
Cancer cells
1:3000, overnight at 4 C
5% milk
1:3000, one hour at room temperature
NA
ECL and film
30 ugs of protein samples were used for this western blot to detect tubulin in PC3 cell lysates. I observed a very strong band using anti-tubulin antibody around 55KD molecular weight.
None
Very good antibody for western blot
Good for western blotting as a loading control as a marker of cytoplasmic fraction.