Anti-DYNC1ILI1 Antibody in Immunofluorescence

Tumor Biology
German Cancer Research Center
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Atlas Antibodies-Sigma

Product Name:

Anti-DYNC1LI1

Catalog Number:

HPA035013

The main goal of our research is to identify new genes that play critical roles in cancer formation and development. To characterize the candidate genes and examine their cellular functions, we perform several cell-based assays. Having a specific antibody that is able to detect the target protein is a fundamental point in our experimental model.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Caco-2 cells

Primary Incubation

1:500 diluted in 1xPBS, incubation for 1h at room temperature

Blocking Agent

No blocking

Secondary Incubation

Cy3-coupled secondary antibody, 30 min incubation at room temperature

Tertiary Incubation

NA

Detection

Fluorescence microscopy

Results Summary

Specific cytoplasmic staining for DYNC1LI1 in Caco-2 cells.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Very nice staining

The Bad

Nothing

The Bottom Line

Great antibody for the detection of DYNC1LI1 with immunofluorescence.

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