Novus Bio
Goat Anti-Mouse IgG1 heavy chain (HRP)
ab97240
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Browse Similar Products: IgG1 Secondary Antibodies
This antibody is super specific! It does not react with mouse IgG2a or human IgG, or any IgG light chain I have tested so far. I use it because I work with mouse tumor lysate in western blot. All tumor lysate contains blood, so IgG is always in the lysates. When my protein of interest is at the same molecular weight of IgG light chain, and the primary antibody is mouse IgG1, I use this antibody. The purpose of the western blot I show here is to verify if this antibody reacts with the light chain of mouse IgG1 specifically, so there is no primary antibody used. The antibody is quite clean. The image I show is over-exposed with some background in order to confirm there is absolutely no IgG light chain signal.
Western Blot
Mouse tumor lysates and mouse IgG1, mouse IgG2a, human IgG
No primary antibody used
5% milk in TBS buffer, 1 hour at room temperature
1:3000 in TBST buffer, 1 hour at room temperature
NA
ECL incubate at room temperature for 1 min
The purpose of the western blot I show here is to verify if this antibody reacts with the light chain of mouse IgG1 specifically, so there is no primary antibody used. The antibody is quite clean. The image I show is over-exposed with some background in order to confirm there is absolutely no IgG light chain signal.
None
This antibody is super specific!
Not found so far.
I like this 2nd antibody because it specifically reacts with mouse IgG1 heavy chain, which is the same as claimed by the data sheet.