Thermo Fisher Scientific
Fast SYBR® Green Master Mix
4385610
I transfected 293T cells to knock down AKT by transient transfection. After 72 hours, I checked the knock down by quantifying the mRNA using SYBR green master mix.
Real time quantitative PCR
239T cells
I prepared the total RNA from all samples, then cDNA was synthesized and qPCR reaction was set up using sybr green master mix. GAPDH was used as an internal loading control.
Minimize pipetting errors by using different tips for each pipetting.
I got the specific amplification of the target gene and it worked absolutely fine.
None
Quick and reliable quantification
It is a good kit for qPCR. I would recommend using this for routine qPCR experiments.
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