Santa Cruz Biotechnology
β-Actin Antibody (C4)
sc-47778
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I use beta HRPO conjugated antibody as a loading control for all my Western blotting. I look for several cell cycle molecules in diffferent cell lines of various cancer origin. I prefer HRPO conjugated beta actin antibody because it's quick, reliable and highly reproducible.
Western Blot
Whole cell lysates of different cell lines
1:20000 dilution in 5% non-fat dry milk in TBST: incubated for 1 hour at RT on rocker shaker
5% non fat dry milk in TBST
Not required
ECL
Beta actin is used as a loading control and clearly shows the equal loading in diferent cell lines.
None
Specificity, less incubation time, reproducibility
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