Mouse IL-1 Protein Tried in Rabbit Disc Cell Culture

ORTHOPAEDIC SURGERY
RUSH UNIVERSITY MEDICAL CENTER
POSTDOC

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Quality of Results

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Company:

BioLegend

Product Name:

Mouse IL-1

Catalog Number:

575102

Info: View Product View Product Specs

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This murine IL-1 protein was used to stimulate the cultured rabbit disc cell culture. Both human IL-1 and murine IL-1 protein were tried on the cells and both worked. Human IL-1 is little stronger than murine IL-1 by stimulating cultured disc cells.

Experimental Design and Results Summary

Applications

ELISA

Sample

Cultured rabbit disc cells

Primary Incubation

Treat cells in culture media for 24h at 100ng/ml concentration

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

ELISA

Results Summary

This murine IL-1 protein worked on rabbit cultured disc cells, less potent than human IL-1 in this specific cell culture.

Additional Notes

None

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Summary

The Good

Easy to handle and very clear information

The Bad

Not as potent as human IL1 protein in terms of rabbit disc cell culture is concerned

The Bottom Line

This protein worked.

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