Senescence ß-Galactosidase Staining Kit for Cells In Vitro

University of Rochester
Environ. Med.
Postdoc

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Company:

Cell Signaling Technology

Product Name:

Senescence β-Galactosidase Staining Kit

Catalog Number:

#9860

We used this SA-beta-gal staining kit for determining senescence in cultured mouse bone marrow derived MSCs. We were able to detect SA-beta gal positive cells after culturing them in MSC culture medium.

Experimental Design and Results Summary

Application

SA-Beta-galactosidase staining

Starting Material

Mouse bone marrow derived MSCs

Protocol Overview

Follow the protocol described in the datasheet provided with the staining kit.http://www.cellsignal.com/products/cellular-assay-kits/senescence-b-galactosidase-staining-kit/9860

Tips

Follow the protocol as per the datasheet

Results Summary

Cultured mouse MSCs were showing SA-beta staining which is indicative of cellular senescence.

Additional Notes

None

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Summary

The Good

Very good staining kit for cell culture experiments

The Bad

None noted

The Bottom Line

Works well for detection of SA-beta-galactosidase positive cells in culture.

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