Cell Signaling Technology
Phospho-PKD/PKCµ (Ser744/748) Antibody
2054
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The aim of our study was to test the time course effect of growth factor on Phospho-PKD/PKCµ (Ser744/748) levels in mammalian cell line. For this, quiesced cells were treated with PDGF, proteins were extracted and resolved in 7.5% SDS-PAGE. Resolved proteins were western transferred on a nitrocellulose membrane and probed for Phospho-PKD/PKCµ (Ser744/748).
Western Blot
Whole cell lysate
1:1000 (5%BSA in TBST), Overnight at 4 degrees Celsius
5% Skimmed milk in TBST for 2h
1:4000 (5%skimmed milk in TBST), 2h at RT
NA
ECL
This antibody worked very badly and a lot of non-specific bands were detected.
None
No background noise
Antibody is not specific for its target
Bad antibody.