Cell Signaling Technology
MeCP2 (D4F3) XP ® Rabbit mAb from Cell Signaling Technology
3456P
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The long-term goal of our lab is to understand the molecular and genetic mechanisms underlying brain development and disases. My focus of work is studying the critical role of MeCP2 (methyl-CpG binding protein 2), whose mutation causes Rett Syndrome, in the development and function of the nervous system. I discovered a novel phosphorylation site on MeCP2, therefore I want to test whether this phosphorylation modulates activity-dependent binding of MeCP2 to the target DNA. As a first step in this study, I established the experiment of DNA-protein binding upon membrane depolarization, Cell Signaling's MeCP2 antibody was used for MeCP2 ChIP assay.
ChIP
Mouse cortical neurons
4ug per ChIP sample for 12 hours at 4°C
3% BSA, 50mM blocking DNA and 0.1% Triton 100 in TBS buffer
NA
Real-time PCR
MeCP2 strongly binds to exon IV of bdnf gene, and weakly binds to exon VI. Upon KCl-induced membrane depolarization, MeCP2 is released from the exon IV, suggesting the this process is activity-dependent.
None
Easy to use
The antibody can enrich MeCP2-binding DNA sequence in ChIP assay.