BioLegend
Brilliant Violet 605 anti-Mouse B220
103243
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The aim of the research is to study splenic hemopoietic progenitor cells in cancer. In my experiment, I tested if a cytokine associated with tumor promotion alters progenitors counts. We selected Brilliant Violet 605™ anti-mouse B220 from BioLegend specifically because B220 is expressed in B cells and can be found in adult spleens. Using this product I was able to exclude differentiated subsets and focus on progenitor cells.
Flow Cytometry
Spleen
1:200 30' on ice
Fcblock
None
FC
No need to define the best working concentration. Bright and easy to compensate with PE, BV-510 and BV-711
Easy
So few ul in each tube
Go for it