LifeSpan BioSciences
MRE11 Mouse anti-Human Monoclonal (aa182-583) (12D7) Antibody
LS-B1700-50
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Human colon cancer cell line was stained with MRE11 at 1/2000 together with nuclear marker DAPI. The cells were PFA fixed, blocked in normal goat serum and then incubated with the antibody for 1 hour. An Alexa conjugated goat polyclonal antibody was used as the secondary antibody. The image was acquired by confocal microscopy.
Immunofluorescence
Human colon cancer cell line
1:2000 for 12hr at 4C
3% serum plus 0.3% Triton-X 100 in PBS
Alexa Fluor 488 Goat Anti-Mouse IgG (H+L) 1:1000 for 1hr at 25C
Not used
Confocal imaging
Mre11 antibody counter-stained with nuclear marker DAPI. The nuclear part of the human colon cancer cell was labeled, which consistent with its role as a component of the MRN complex, acting in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis.
None
Highly specific, no background staining
This is a very good antibody to detect Mre11 in the cancer cell line