Assessing the quality and quantity of nucleic acids is critically important to successful next-generation sequencing (NGS). Optimal sequencing performance requires careful preparation of NGS libraries with nucleic acids of sufficient quality. To identify suitable nucleic acids, leading NGS companies recommend checking nucleic acid quality prior to library preparation. An initial quality check helps find samples likely to produce low performing NGS libraries due to fragmentation or degradation. Similarly, confirming the size, concentration, and overall quality of prepared libraries helps optimize cluster generation during sequencing and aids in both cost and time savings while maximizing NGS data quality and output.
The Fragment Analyzer and the FEMTO Pulse streamline nucleic acid quality assessment during NGS library preparations, ultimately improving result generation through improved and optimized workflows. Data will be presented that demonstrates how sample quality can impact NGS outcomes and why accurate quantification of nucleic acids is important.
You will learn about:
- Why QC is important to successful sequencing
- Instruments that can accurately assess nucleic acid quality and quantity
- How improper handling of nucleic acids affects results
Who should attend:
- Genomics researchers
- Sequencing core lab managers/technicians
- Molecular biologists
- Cancer researchers