CRISPR has already infiltrated every sector of our lives.
New methods elicit targeted changes to the genome without DSBs
Cas9 has been a game-changer but it's not the only nuclease variant available
Early-career scientists share experimental details and more.
CRISPR is being used now to improve human health
sgRNAs can now be chemically synthesized at an affordable cost.
Recent improvements to system help reduce off-target activity.
Easy to design and use system has proven to be widely accessible.
Others techs have benefits but CRISPR has been game-changing.
A guide to the tools/techniques available to help you ensure success.
ZFN, TALENS, and CRISPR-Cas9 have ushered in a new era in biology.