The Agilent Seahorse XF Flex Analyzer, paired with the XF Flex 3D Capture Microplate-L, advances metabolic research by enabling sensitive, consistent analysis of 3D models like tissue slices, organoids, and small organisms. These models offer more physiologically relevant insights into complex biology. Tissue slices, in particular, are valued as a model system for their accessibility, cost-effectiveness, and preservation of native architecture. Download this technical overview to explore an optimized workflow for measuring oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) in live liver tissue, providing real-time metabolic data with improved sensitivity and reliability.