This protocol describes the detailed procedure of the setup and use of a packed-bed Fibra-Cel® Disk bioreactor controlled by a BioFlo 320® control station. Fibra-Cel® Disks are proven support systems used to increase yields of anchorage-dependent cell cultures. When combined with the Eppendorf packed-bed impeller system, the Fibra-Cel® Disks create a three-dimensional matrix that immobilizes cells within the bioreactor. This application note guides users through all steps of the bioprocess, starting from setting up the packed-bed Fibra-Cel® bioreactor to the preparation and operation of a perfusion culture. Download now to learn more.
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