Webinar Date: June 15, 2021
Time: 9 a.m. AEST (6/16), 4 p.m. PT (6/15), 7 p.m. ET (6/15)
New research is challenging the classical view of hematopoiesis—in which the earliest commitment toward the lymphoid lineage is described within a multipotent progenitor population termed lymphoid-primed multipotent progenitors (LMPPs). These cells are Lin–cKithiSca1+Flt3+ and devoid of erythroid and megakaryocytic potential but still produce myeloid cells.
In this webinar, Sara Tomei of Shalin Naik’s group at The Walter & Eliza Hall Institute, will discuss the group’s recently identified subpopulation of LMPPs marked by Dach1-GFP downregulation. This subpopulation, termed lymphoid primed progenitors (LPPs), is completely devoid of any myeloid potential but can produce B and T cells. She will also explain how the team used LEGENDScreen™ to identify potential surface markers that could act as surrogates for LPPs.
Through staining for 263 markers, the group identified CD69 as a novel candidate marker expressed on this population. This crucial finding allows any researcher to prospectively isolate this population without the need for Dach1-GFP reporter mice.
In this webinar you’ll learn:
- The significance of lymphoid primed progenitors (LPPs) in hematopoietic stem and progenitor populations
- How to isolate lymphoid primed progenitors (LPPs) without a Dach1-GFP reporter mouse
- Lessons learned running LEGENDScreen™, and how other novel markers of heterogeneity can be revealed through this data set
Who should attend:
- Stem cell researchers
- Immunologists
- Single-cell systems biologists
- Hematopoiesis researchers