Adventures with Imaging Flow Cytometry: Aging Cells, Giant Viruses and Golgi Fragmentation

In this webinar we will describe three recent applications for imaging flow cytometry (IFC), a technology that combines the information-rich imagery of microscopy with the quantification and high-throughput abilities of flow cytometry. First we describe an automated method to quantify the senescence phenotype (a permanent state of cell cycle arrest), combining it with additional markers, improving the current qualitative methods. The second part describes using IFC to monitor changes in Acanthamoeba polyphaga cells infected with the giant DNA virus Mimivirus, including generation of viral factories, transport and fusion of replication centers within the cell, accumulation of viral progeny, and changes in cell morphology. The third part describes development of a method for using IFC to automatically quantify Golgi fragmentation, which plays an important role in numerous cellular processes. These examples demonstrate the diversity and versatility of IFC.