Luciferase Assay System with Reporter Lysis Buffer E4030 from Promega

Product Specs
ItemLuciferase Assay System with Reporter Lysis Buffer
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE4030
ReactivityMammalian, Bacteria, Plant
Detection TargetLuciferase
SpeciesMammalian, plant.&bacteria
Quantity100 assays
MethodLuciferase
Sample TypeMammalian, cell , plant.&bacteria cells
SensitivityTo 10^{–20
moles of luciferase.}
Assay Time5 minutes
Molecular Weight~44kDa

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Localized cardiac small molecule trajectories and persistent chemical sequelae in experimental Chagas diseaseNature CommunicationsOctober 25, 2023Zongyuan Liu, Rebecca Ulrich vonBargen, April L. Kendricks, Kate Wheeler, Ana Carolina Leão, Krithivasan Sankaranarayanan, Danya A. Dean, Shelley S. Kane, Ekram Hossain, Jeroen Pollet, et al.1000×g for 10 min, then diluted in 1X Cell Culture Lysis Reagent (Promega Luciferase Assay system, cat # E4030) to a final concentration of 1 × 106 epimastigotes/mL. Parasites were lysed by freezing on dry ice,

(2) Enhancement of Neutralization Responses through Sequential Immunization of Stable Env Trimers Based on Consensus Sequences from Select Time Points by Mimicking Natural InfectionInt J Mol SciAugust 10, 2023Mingming Wan, Xiao Yang, Jie Sun, Elena E. Giorgi, Xue Ding, Yan Zhou, Yong Zhang, Weiheng Su, Chunlai Jiang, Yaming Shan, et al.cells were washed with PBS once and lysed in Reporter Lysis Buffer (Promega, Madison, WI, USA, Cat. E4030). Luciferase activity was measured on a Victor X Multilabel Readers (PerkinElmer, Waltham, MA, USA).

(3) Monoterpenoid aryl hydrocarbon receptor allosteric antagonists protect against ultraviolet skin damage in female miceNature CommunicationsMay 11, 2023Karolína Ondrová, Iveta Zůvalová, Barbora Vyhlídalová, Kristýna Krasulová, Eva Miková, Radim Vrzal, Petr Nádvorník, Binod Nepal, Sandhya Kortagere, Martina Kopečná, et al.Cells were grown to 90% confluency in a 60 mm dish. After removal of the medium, 1 mL of lysis buffer (E4030, Promega) was applied for 10 min on ice. Cells were scraped, sonicated, and centrifuged at 15,900 ×

(4) HIV Nef Expression Down-modulated GFAP Expression and Altered Glutamate Uptake and Release and Proliferation in AstrocytesAging and diseaseFebruary 1, 2023Kelly M. Wilson, Johnny J. HeThe cells continued to culture for 48 hr, rinsed in ice-cold PBS, and then lysis buffer added (Cat. # E4030, Promega, Madison, WI). The cells were incubated at 4°C for 10 min, scraped off, collected in a 1.5

(5) Stimulating TAM-mediated anti-tumor immunity with mannose-decorated nanoparticles in ovarian cancerBMC CancerDecember 1, 2022Evan B. Glass et al.luminescent measurements. Samples were prepared for luminescence readings using a Luciferase Assay System (Promega; E4030) following the manufacturer’s instructions. In vivo biodistribution studies in ovarian tumor-bearing

(6) CK2-mediated phosphorylation of SUZ12 promotes PRC2 function by stabilizing enzyme active siteNature communicationsNovember 9, 2022Gong L, Liu X, Jiao L, Yang X, Lemoff A, Liu X.transfection. The luciferase activity was then measured using the Luciferase Assay System kit (Promega, Cat No. E4030). Luciferase signals were normalized by β- galactosidase activity using the β-galactosidase Enzyme Assay

(7) Payload distribution and capacity of mRNA lipid nanoparticlesNature CommunicationsSeptember 23, 2022Sixuan Li, Yizong Hu, Andrew Li, Jinghan Lin, Kuangwen Hsieh, Zachary Schneiderman, Pengfei Zhang, Yining Zhu, Chenhu Qiu, Efrosini Kokkoli, et al.liver and spleen were then harvested, weighted, and disgested by reporter lysis buffer (Promega, Cat# E4030) assisted by an ultrasonic processor (Qsonica, Cat# Q55A). The digested solution was subjected to a

(8) Secretagogin is a Ca(2+)-dependent stress-responsive chaperone that may also play a role in aggregation-based proteinopathiesThe Journal of biological chemistrySeptember 1, 2022Chidananda AH, Khandelwal R, Jamkhindikar A, Pawar AD, Sharma AK, Sharma Y.Luciferase construct in basic pGL3 vector [procured from Promega luciferase assay system kit (Catalog No. E4030)], pEGFP(N3)-SCGN plasmid, and SCGN-eGFPN3 constructs were used for transient overexpression in HEK-293T cells

(9) miR-451a Regulates Neuronal Apoptosis by Modulating 14-3-3ζ-JNK Axis upon Flaviviral InfectionmSphereAugust 31, 2022Chakraborty S, Basu A.harvested and subjected to luciferase assay using luciferase assay system (Promega, catalogue number: E4030) according to manufacturer’s recommendation. Firefly-luciferase activity has been normalized using renilla-luciferase

(10) Immunogenicity of an AAV-Based COVID-19 Vaccine in Murine Models of Obesity and AgingVirusesApril 15, 2022Dawid Maciorowski, Cheikh Diop, Urja Bhatt, Reynette Estelien, Dan Li, Ruchi Chauhan, Luk H. Vandenberghe, Nerea Zabaletathen incubated for 48 h. After incubation, cells were lysed with reporter lysis buffer (Promega, Cat# E4030). These plates were frozen at −80 °C for 60 min to enhance lysis. Thereafter, they were thawed at 37

(11) A novel peptide inhibitor of Dll4-Notch1 signalling and its pro-angiogenic functionsBritish journal of pharmacologyApril 1, 2022Zhu G, Lin Y, Ge T, Singh S, Liu H, Fan L, Wang S, Rhen J, Jiang D, Lyu Y, et al.TAT-Sc or TAT-ANK for another 12 h. A luciferase assay system with reporter lysis buffer (Promega, E4030) was used to detect the luciferase activity. β-Gal activity was measured by β-gal enzyme assay system

(12) Analysis of DNA Repair Using Transfection-Based Host Cell ReactivationMolecular Toxicology ProtocolsMarch 31, 2022Jennifer M. Johnson, Jean J. LatimerQuantification of Luciferase Expression Author Manuscript 1. Luciferase® Assay System (Promega, cat. no. E4030). 2. Luciferase assay reagent (LAR): resuspend the lyophilized luciferase assay substrate in 10 mL

(13) Biallelic, Selectable, Knock-in Targeting of CCR5 via CRISPR-Cas9 Mediated Homology Directed Repair Inhibits HIV-1 ReplicationFrontiers in ImmunologyMarch 21, 2022Stefan H. Scheller, Yasmine Rashad, Fayez M. Saleh, Kurtis A. Willingham, Antonia Reilich, Dong Lin, Reza Izadpanah, Eckhard U. Alt, Stephen E. Brauncultured for 48 hours before lysis with 1x Reporter Lysis Buffer (Promega, Madison, Wisconsin, Cat. # E4030). The cell lysate was centrifuged at 20000 × g for 10 min and 20 μl of the supernatant were mixed with

(14) Mutations that adapt SARS-CoV-2 to mink or ferret do not increase fitness in the human airwayCell reportsFebruary 8, 2022Zhou J, Peacock TP, Brown JC, Goldhill DH, Elrefaey AME, Penrice-Randal R, Cowton VM, De Lorenzo G, Furnon W, Harvey WT, et al.Cap Analogue,Promega,P1711 TMB substrate,Europa Bioproducts,MO701A reporter lysis buffer,Promega,E4030 Coelenterazine-H,Promega,S2011 ,, Critical commercial assays,Critical commercial assays,Critical

(15) Crosstalk between macrophage-derived PGE(2) and tumor UHRF1 drives hepatocellular carcinoma progressionTheranosticsJanuary 1, 2022Zhang J, Zhang H, Ding X, Hu J, Li Y, Zhang J, Wang H, Qi S, Xie A, Shi J, et al.were measured 48 hours after transfection using a Luciferase Assay System with Reporter Lysis Buffer (E4030, Promega Corporation, USA). PGE2 (200 ng/mL) or supernatant treatments were performed 24 hours before

(16) The Sall2 transcription factor promotes cell migration regulating focal adhesion turnover and integrin β1 expressionFrontiers in cell and developmental biologyJanuary 1, 2022Riffo E, Palma M, Hepp MI, Benítez-Riquelme D, Torres VA, Castro AF, Pincheira R.vector control per well. After 24 h, the transfected cells were lysed with reporter lysis 5X buffer (E4030; Promega, Madison, WI, United States) and centrifugated at 14000 × g to pellet cell debris. The supernatant

(17) Chandipura virus dysregulates the expression of hsa-miR-21-5p to activate NF-κB in human microglial cellsJournal of Biomedical ScienceDecember 1, 2021Neha Pandey, Meghana Rastogi, Sunit K. Singhtransfection at MOI 0.1. The luciferase expression was studied by using the Luciferase assay kit (#E4030; Promega) as per the manufacturer’s protocol and the luminescence activity was measured on the multimode

(18) Development of CIDEA reporter mouse model and its application for screening thermogenic drugsScientific reportsSeptember 16, 2021Son Y, Choi C, Song C, Im H, Cho YK, Son JS, Joo S, Joh Y, Lee YJ, Seong JK, et al.assay Luciferase activity of tissue or cell lysate was analyzed using Luciferase Assay System (Promega, E4030) with a microplate luminometer (Centro LB 960, BERTHOLD), according to the manufacturer’s instructions.

(19) Y chromosomal noncoding RNAs regulate autosomal gene expression via piRNAs in mouse testisBMC biologySeptember 9, 2021Reddy HM, Bhattacharya R, Tiwari S, Mishra K, Annapurna P, Jehan Z, Praveena NM, Alex JL, Dhople VM, Singh L, et al.medium. The cell extracts were prepared 24 h post transfection using Reporter Lysis Buffer (Promega Cat # E4030) and assayed for Luciferase activity in EnSpire 2300 multimode plate reader (Perkin Elmer). The Luciferase

(20) An AAV-based, room-temperature-stable, single-dose COVID-19 vaccine provides durable immunogenicity and protection in non-human primatesCell host & microbeSeptember 8, 2021Zabaleta N, Dai W, Bhatt U, Hérate C, Maisonnasse P, Chichester JA, Sanmiguel J, Estelien R, Michalson KT, Diop C, et al.incubated for 48 hours. After 48 hours, cells were lysed with Reporter Lysis Buffer (Promega, Cat# E4030). These plates were frozen at -80°C for 60 minutes. Thereafter, they were thawed at 37°C for 20 mins

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1/22/2015Easy to use detection system Yonghou JiangThis research plan is designed to evaluate nanoparticle-based vaginal drug delivery systems for HIV prevention. The experimental focus is to achieve protection against vaginal transmission of HIV-1 by... Read Review

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Luciferase Assay System with Reporter Lysis Buffer

Luciferase Assay System with Reporter Lysis Buffer from Promega

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Promega

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Luciferase Assay System by Promega

Luciferase Assay System From Promega

Luciferase Assay System From Promega

Luciferase Assay System with Reporter Lysis Buffer from Promega

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