Prostaglandin E Metabolite ELISA Kit 514531 from Cayman Chemical

Product Specs
ItemProstaglandin E Metabolite ELISA Kit
CompanyCayman Chemical
Price Pricing Info Supplier Page View Company Product Page
Catalog Number514531
Quantity480 Well
ApplicationsELISA
Detection Range0.39-50 pg/ml
DetectionColorimetric
MethodEIA
Sensitivity80% B/B0: ~2 pg/ml
Research AreaBone Growth & Remodeling, Cyclooxygenase Pathway, Endocrinology & Metabolism, Immunology & Inflammation, Inflammatory Lipid Mediators, Lipid Biochemistry, Prostaglandins

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Cayman Chemical

Cayman Chemical Company 1180 East Ellsworth Road

Ann Arbor, Michigan 48108

United States

Phone: (800) 364-9897
(734) 971-3640

Company Profile

Website: http://www.caymanchem.com/

(1) Effect of Hibiscus sabdariffa L. on the Metabolism of Arachidonic Acid in the Isolated Kidney of a Rat Model of Metabolic SyndromeInternational Journal of Molecular SciencesSeptember 18, 2023Israel Pérez-Torres, María Elena Soto, Linaloe Manzano-Pech, Eulises Díaz-Díaz, Raúl Martínez-Memije, Juan Carlos Torres-Narváez, Verónica Guarner-Lans, Vicente Castrejón-Téllezsaline–ethanol (2:1 vol/vol) [14]. The prostaglandins were determined with ELISA kits; prostaglandin E (Item No. 514531), 6-keto prostaglandin F1α (Item No. 515211), leukotriene B4 (Item No. 520111), and thromboxane B2 (Item

(2) Circumvention of luteolysis reveals parturition pathways in mice dependent upon innate type 2 immunityImmunityMarch 14, 2023Siewiera J, McIntyre TI, Cautivo KM, Mahiddine K, Rideaux D, Molofsky AB, Erlebacher A.Kit,Cayman Chemical,Cat# No. 514010 Prostaglandin E Metabolite ELISA Kit,Cayman Chemical,Cat# No. 514531 iScript TM cDNA Synthesis Kit,Bio-Rad,Cat#170–8891 eBioscience™ Foxp3 / Transcription Factor Staining

(3) Elevated Levels of PGE2-Metabolite in Cerebrospinal Fluid and Cox-2 Gene Polymorphisms in Patients with Chronic, Post Cholecystectomy Pain and Visceral Hyperalgesia Compared to Healthy Controls. A Hypothesis-Generating Pilot StudyJournal of Pain ResearchDecember 14, 2022Morten Rune Blichfeldt-Eckhardt, Dorte Aalund Olsen, Rikke Fredslund Andersen, Palle Toft, Laila Bendixconcentrations8,36 and were measured using a commercially available competitive assay from Cayman (PGEM 514531, Cayman Chemical, Ann Arbor, MI, USA). The analyses were performed according to the manufacturer’s protocols.

(4) The Surface Area to Volume Ratio Changes the Pharmacokinetic and Pharmacodynamic Parameters in the Subcutaneous Tissue Cage Model: As Illustrated by Carprofen in SheepFrontiers in Veterinary ScienceJuly 1, 2022Richard Munn, Ted Whittem, Andrew P. Woodwardprocessed using commercial ELISA kits (Cayman Chemical; Prostaglandin E Metabolite ELISA Kit Item No. 514531 and Thromboxane B2 ELISA Kit Item No. 501020). Thromboxane concentrations were measured in serum samples

(5) Clinical and biochemical characteristics of 12 Chinese primary hypertrophic osteoarthropathy patients with HPGD mutationsInternational Journal of Biological SciencesJune 6, 2022Qi Lu, Yang Xu, Shanshan Li, Zeng Zhang, Jiagen Sheng, Zhenlin Zhangwere normalized to creatinine according to the manufacturer's instructions (Item 500141 for PGE2, Item 514531 for PGE-M, Item 500701 for creatinine; Cayman Chemicals, Ann Arbor, MI, USA). Statistical analysis Normally

(6) Differential effects of cyclo-oxygenase 1 and 2 inhibition on angiogenesis inhibitor-induced hypertension and kidney damageClinical ScienceMay 13, 2022Katrina M. Mirabito Colafella et al.Kit No. 514010, Cayman Chemical) and its metabolite, PGE-M (Prostaglandin E Metabolite ELISA Kit No. 514531, Cayman Chemical). Markers of oxidative stress To assess oxidative stress, aortic and renal superoxide

(7) Comparison of lidocaine alone or in combination with a local nerve block of ethanol, bupivacaine liposome suspension, or oral meloxicam to extend analgesia after scoop dehorning in Holstein calvesJDS CommunicationsMay 1, 2022Miriam S. Martin, Michael D. Kleinhenz, Abbie V. Viscardi, Shawnee R. Montgomery, Charley A. Cull, Jon E. Seagren, Kelly F. Lechtenberg, Johann F. Coetzeerespectively. Prostaglandin E2 metabolites were analyzed using a commercially available ELISA kit (cat. no. 514531; Cayman Chemical) following manufacturer specifications with minor modifications, as described in Martin

(8) HDAC3-Regulated PGE2 Production by Microglia Induces Phobic Anxiety Susceptibility After Stroke and Pointedly Exploiting a Signal-Targeted Gamma Visual Stimulation New TherapyFrontiers in ImmunologyFebruary 18, 2022Hongrui Zhu, Yi Guo, Ailing Huang, Huidan Shen, Yang Chen, Jingyi Song, Ao Guan, Liang Wu, Huiting Wang, Bin Dengsupernatant was measured by a PGE2 ELISA kit according to the manufacturer’s protocol (Cayman Chemical, 514531). RNA-Seq Analysis RNA-seq was carried out by the UCLA Neuroscience Genomics Core (UNGC). Samples

(9) Assessment of diagnostic accuracy of biomarkers to assess lung consolidation in calves with induced bacterial pneumonia using receiver operating characteristic (ROC) curvesJournal of Animal ScienceJanuary 1, 2022Miriam Martin, Michael D Kleinhenz, Shawnee R Montgomery, Dale A Blasi, Kelli M Almes, Angela K Baysinger, Johann F CoetzeePGEM. Prostaglandin E2 metabolites were analyzed using a commercially available ELISA kit (cat. no. 514531, Cayman Chemical, Ann Arbor, MI) following manufacturer specifications with minor modifications. Sample

(10) Effect of bupivacaine liposome suspension administered as a local anesthetic block on indicators of pain and distress during and after surgical castration in dairy calvesJournal of Animal ScienceJanuary 1, 2022Miriam S Martin, Michael D Kleinhenz, Abbie V Viscardi, Andrew K Curtis, Blaine T Johnson, Shawnee R Montgomery, Maria E Lou, Johann F Coetzee20.86%. Prostaglandin E2 metabolites PGEMs were analyzed using a commercially available ELISA kit (cat. no. 514531, Cayman Chemical, Ann Arbor, MI) following the manufacturer’s specifications with minor modifications.

(11) Multidrug resistance‐associated protein 4 (Mrp4) is a novel genetic factor in the pathogenesis of obesity and diabetesThe FASEB JournalNovember 13, 2021Ajay C. Donepudi, Yoojin Lee, Ji‐Young Lee, John D. Schuetz, José E. Manautoulevels in WT and Mrp4−/− mice were quantified using Prostaglandin E Metabolite ELISA Kit (Catalog: 514531; Cayman Chemicals) (28). Indirect calorimetry analysis: WT and Mrp4−/− female mice ( 5 WT mice and

(12) 2-Methoxyestradiol Ameliorates Angiotensin II–Induced Hypertension by Inhibiting Cytosolic Phospholipase A ₂ α Activity in Female MiceHypertensionNovember 1, 2021Chi Young Song, Purnima Singh, Mustafa Motiwala, Ji Soo Shin, Jessica Lew, Shubha R. Dutta, Frank J. Gonzalez, Joseph V. Bonventre, Kafait U. Malikthe concentration of its metabolites using a Prostaglandin E Metabolite ELISA kit (Cayman Catalog No. 514531) following the manufacturer’s instructions. This kit enables the conversion of 13,14-dihydro-15-keto

(13) 6β-Hydroxytestosterone Promotes Angiotensin II-Induced Hypertension via Enhanced Cytosolic Phospholipase A ₂ α ActivityHypertensionOctober 1, 2021Purnima Singh, Chi Young Song, Shubha R. Dutta, Ajeeth Pingili, Ji Soo Shin, Frank J. Gonzalez, Joseph V. Bonventre, Kafait U. Malikconcentrations of PGE2 metabolites in urine using a Prostaglandin E Metabolite EIA Kit (Cayman Catalog No. 514531), as per the manufacturer’s instructions. This kit enables the conversion of 13, 14-dihydro-15-keto

(14) Time-of-Day-Dependent Gating of the Liver-Spinal Axis Initiates an Anti-Inflammatory Reflex in the RateNeuroNovember 1, 2020Soto-Tinoco E, Santacruz E, Basualdo-Sigales MDC, Guerrero-Vargas NN, Buijs RM.metabolite of PGE2 since the latter is too unstable to be measured directly (Cayman Chemical, catalog #514531, RRID:AB_2876793). Immunohistochemistry Following perfusion, spinal cord, brain and brainstem were

(15) Evaluating the utility of a CO2 surgical laser for piglet castration to reduce pain and improve wound healing: a pilot studyJournal of Animal ScienceNovember 1, 2020Abbie V Viscardi, Charley A Cull, Michael D Kleinhenz, Shawnee Montgomery, Andrew Curtis, Kelly Lechtenberg, Johann F Coetzee0.5 hr postcastration. A commercially available PGEM ELISA kit was used (Cayman Chemical catalog no. 514531; Ann Arbor, MI) with minor modifications. Briefly, samples were purified by adding 1.5 mL ice cold acetone

(16) Inhibition of viral and bacterial trigger-stimulated prostaglandin E₂ by a throat lozenge containing flurbiprofen: An in vitro study using a human respiratory epithelial cell lineSAGE Open MedicineSeptember 24, 2020Rob Lambkin-Williams, Alex Mann, Adrian Shephardenzyme-linked immunosorbent assay (ELISA) using a prostaglandin E metabolite enzyme immunoassay kit (Item No. 514531, Cayman Chemical,27 Cambridge, UK), according to the manufacturer’s methodology. Developed plates were

(17) Differences in cNOS/iNOS Activity during Resistance to Trypanosoma cruzi Infection in 5-Lipoxygenase Knockout MiceMediators of InflammationOctober 24, 2019Carolina Panis, Vanessa Jacob Victorino, Vera Lúcia Hideko Tatakihara, Rubens Cecchini, Luiz Vicente Rizzo, Lucy Megumi Yamauchi, Sueli Fumie Yamada-Ogatta, Marli Cardoso Martins-Pinge, Phileno Pinge-Filhothe acetylcholinesterase-based assay PGE2 EIA kit and leukotriene B4 EIA kit (Cayman Chemical, CAT#514531). 2.7. Cardiac Parasitism. The mouse hearts were removed on day 12 i.p., sliced transversally in three

(18) Early B Cell Factor 1 (EBF1) Regulates Glomerular Development by Controlling Mesangial Maturation and Consequently COX-2 ExpressionJournal of the American Society of NephrologySeptember 1, 2019Tracy Nelson, Heino Velazquez, Nancy Troiano, Jackie A. Fretzthe kit from Sigma (#MAK080). ELISAs from Cayman Chemical were used to measure PG:PGE metabolites (#514531), 11-dehydro TBXB2 (#519510), PGI metabolites (#501100), 13,14-dihydro-15-keto PGF2a (#516671), and JASN

(19) Prostaglandin E₂Inhibits the Ability of Neutrophils to KillListeria monocytogenesThe Journal of ImmunologyJune 15, 2019Michelle G. Pitts, Sarah E. F. D’Orazioenzyme immunoassay Prostaglandin E2 was measured using the Prostaglandin E2 Metabolite (PGEM) EIA kit (Cayman Chemical No. 514531), which converts all the metabolites of PGE2 present in a sample into a single stable derivative prior

(20) Bone changes after short-term whole body vibration are confined to cancellous boneJ Musculoskelet Neuronal InteractDecember 5, 2018William O. Runge, David S. Ruppert, Denis J. Marcellin-Little, Laurence E. Dahners, Ola L.A. Harrysson, Paul S. Weinholduntil analysis of PGE2 metabolite levels according to the instructions of a commercially available kit (514531, Cayman Chemical Co, Ann Arbor, MI). The 0.0, 0.15, and 1.2 g groups were analyzed. Cartilage thickness

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Prostaglandin E Metabolite ELISA Kit from Cayman Chemical

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Prostaglandin E Metabolite ELISA Kit from Cayman Chemical

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