Fig 1: SH decreased MMP-9 and increased TIMP-1, TIMP-2 levels in both mouse modelSerum samples and section specimen were used to detect these markers. In the 4T1 mouse model, (A) MMP-2, (B) MMP-9, (C) TIMP-1 and (D) TIMP-2 serum levels were tested using ELISA. (E) IHC staining and (F) quantification of MMP-9, TIMP-1 and TIMP-2 expression in 4T1 tumor specimens. (G) IHC staining and (H) quantification of MMP-9, TIMP-1 and TIMP-2 expression in MDA-MB-231 lung specimens. Data are represented as mean ± S.D. of three independent experiments. *P < 0.05, #P < 0.01, SH treated group compared with the untreated control group.