Pacific Blue(TM) Annexin V Apoptosis Detection Kit with 7-AAD 640926 from BioLegend

Product Specs
ItemPacific Blue(TM) Annexin V Apoptosis Detection Kit with 7-AAD
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number640926
Quantity100 tests
ApplicationsFlow Cytometry (FC)
ReactivityHuman, Mouse, Rat
Antibody TypePrimary
NCBI Full Gene Nameannexin A5
NCBI Gene AliasesANX5, CPB-I, ENX2, HEL-S-7, PP4, RPRGL3, VAC-alph
Detection TargetAnnexin V
Conjugate/Tag/LabelPacific Blue

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Cell type-specific delivery by modular envelope designNat CommunAugust 23, 2023Daniel Strebinger, Chris J. Frangieh, Mirco J. Friedrich, Guilhem Faure, Rhiannon K. Macrae, Feng Zhang4 °C. After this incubation, cells were washed and stained with Annexin V-PB450 (BioLegend, Cat. # 640926) for 15 min at room temperature in Annexin V binding buffer according to the manufacturer’s instructions.

(2) The impact of metabolic stressors on mitochondrial homeostasis in a renal epithelial cell model of methylmalonic aciduriaScientific ReportsMay 11, 2023Anke Schumann, Marion Brutsche, Monique Havermans, Sarah C. Grünert, Stefan Kölker, Olaf Groß, Luciana Hannibal, Ute Spiekerkoettercells were stained for Annexin V and 7AAD according to the manufacturer’s protocol (Biolegend, cat# 640926). Separate samples were stained with MitoSpy Green FM (Biolegend, cat# 424805) to determine the mitochondrial

(3) Klebsiella pneumoniae Induces Inflammatory Bowel Disease Through Caspase-11-Mediated IL18 in the Gut Epithelial CellsCellular and molecular gastroenterology and hepatologyJanuary 1, 2023Zhang Q, Su X, Zhang C, Chen W, Wang Y, Yang X, Liu D, Zhang Y, Yang R.RRID:AB_2834604 Anti-villin,Proteintech,Cat: 16488-1-AP RRID:AB_2215975 Antibodies for flow cytometry,, 7-AAD,BioLegend,Cat: 640926 Brilliant Violet 421 anti-mouse CD45 (30-F11),BioLegend,Cat: 103133 RRID:AB_10899570 PE anti-mouse

(4) Meteorin-like is an injectable peptide that can enhance regeneration in aged muscle through immune-driven fibro/adipogenic progenitor signalingNature communicationsDecember 9, 2022Lee DE, McKay LK, Bareja A, Li Y, Khodabukus A, Bursac N, Taylor GA, Baht GS, White JP.Biolegend 127626), APC anti-mouse F4/80 (1:200, Biolegend 123116), Pacific Blue AnnexinV (Biolegend 640926), PE-Cy7 anti-mouse CD11b (1:200, Biolegend 101216). Mouse Meteorin-like DuoSet ELISA (R&D Systems DY6679). Statistical

(5) Miro1 R272Q disrupts mitochondrial calcium handling and neurotransmitter uptake in dopaminergic neuronsFrontiers in Molecular NeuroscienceDecember 2, 2022Lisa Schwarz, Karan Sharma, Lorenzo D. Dodi, Lara-Sophie Rieder, Petra Fallier-Becker, Nicolas Casadei, Julia C. Fitzgeraldtreated with 1 μM Staurosporine for 4 h, dissociated and stained with AnnexinV and 7-AAD (BioLegend, #640926) following the manufacturer’s instructions. Mean fluorescence was measured in phenol red-free maturation

(6) The Phosphofurin Acidic Cluster Sorting Protein 2 (PACS-2) E209K Mutation Responsible for PACS-2 Syndrome Increases Susceptibility to ApoptosisACS OmegaSeptember 27, 2022Rong Xuan Zang, Mitchell J. Mumby, Jimmy D. Dikeakossubjected to viability staining with the Pacific Blue Annexin V Apoptosis Detection Kit with 7-AAD (640926, BioLegend) following the manufacturer’s recommendations. Stained cells were analyzed immediately via

(7) Synergistic Tumor Cytolysis by NK Cells in Combination With a Pan-HDAC Inhibitor, PanobinostatFrontiers in ImmunologyAugust 31, 2021Lukman O. Afolabi, Jiacheng Bi, Xuguang Li, Adeleye O. Adeshakin, Funmilayo O. Adeshakin, Haisi Wu, Dehong Yan, Liang Chen, Xiaochun Wanwas evaluated using Pacific Blue™ Annexin V Apoptosis - 7-aminoactinomycin D (7-AAD) Detection Kit (640926, BioLegend, San Diego, CA). The assay was performed as recommended by the manufacturer. Cells were analyzed

(8) Uncoupling of macrophage inflammation from self-renewal modulates host recovery from respiratory viral infectionImmunityJune 8, 2021Zhu B, Wu Y, Huang S, Zhang R, Son YM, Li C, Cheon IS, Gao X, Wang M, Chen Y, et al.Assay,ImmunoChemistry Technologies,Cat# 9122 Pacific Blue Annexin V Apoptosis Detection Kit,Biolegend,Cat# 640926 Propidium Iodide Flow Cytometry Kit,abcam,Cat# ab139418 BD PharMingen BrdU Flow Kits,BD Biosciences,Cat#

(9) The Heteromeric Complex Formed by Dopamine Receptor D5 and CCR9 Leads the Gut Homing of CD4+ T Cells Upon InflammationCellular and Molecular Gastroenterology and HepatologyApril 20, 2021Francisco Osorio-Barrios, Gemma Navarro, Javier Campos, Valentina Ugalde, Carolina Prado, Iu RaÃ¯ch, Francisco Contreras, Ernesto LÃ³pez, Alexandra Espinoza, Alvaro Lladser, et al.apoptosis/necrosis was determined with a commercial kit (Pacific Blue Annexin V Apoptopsis Detection Kit with 7-AAD; 640926; BioLegend). Study Approval All procedures and housing of mice were compliant with the recommendations

(10) Engineering of Biocompatible Coacervate-Based Synthetic CellsACS Applied Materials & InterfacesFebruary 24, 2021Marleen H. M. E. van Stevendaal, Laurynas Vasiukas, N. Amy Yewdall, Alexander F. Mason, Jan C. M. van Hest100-041) were purchased from LGC standards. Pacific Blue Annexin V Apoptosis Detection Kit with 7-AAD (no. 640926) was purchased from BioLegend. LysoTracker Green DND-26 (no. H3570), Hoechst 33342 (no. H3570), and Live Cell Imaging

(11) TMEM41B Is a Pan-flavivirus Host FactorCellJanuary 7, 2021Hoffmann HH, Schneider WM, Rozen-Gagnon K, Miles LA, Schuster F, Razooky B, Jacobson E, Wu X, Yi S, Rudin CM, et al.for flow cytometry using the Pacific Blue Annexin V Apoptosis Detection Kit with 7-AAD (Biolegend: 640926) according to the manufacturer’s protocol. Cells were subsequently washed twice in binding buffer, followed

(12) TMEM41B is a pan-flavivirus host factorbioRxiv : the preprint server for biologyOctober 11, 2020Hoffmann HH, Schneider WM, Rozen-Gagnon K, Miles LA, Schuster F, Razooky B, Jacobson E, Wu X, Yi S, Rudin CM, et al.for flow cytometry using the Pacific Blue Annexin V Apoptosis Detection Kit with 7-AAD (Biolegend: 640926) according to the manufacturer’s protocol. Cells were subsequently washed twice in binding buffer, followed

(13) Coordinated Viral Control by Cytotoxic Lymphocytes Ensures Optimal Adaptive NK Cell ResponsesCell reportsSeptember 22, 2020Diaz-Salazar C, Sun JC.FAM FLICA Poly Caspase Kit,Bio-Rad,Cat#ICT092 Pacific Blue Annexin V Apoptosis Detection Kit,BioLegend,Cat# 640926 Experimental Models: Organisms/Strains,Experimental Models: Organisms/Strains,Experimental Models:

(14) NK cell defects in X-linked pigmentary reticulate disorderJCI insightNovember 1, 2019Starokadomskyy P, Wilton KM, Krzewski K, Lopez A, Sifuentes-Dominguez L, Overlee B, Chen Q, Ray A, Gil-Krzewska A, Peterson M, et al.death was completed. Briefly, a Pacific Blue Annexin V Apoptosis Detection Kit with 7-AAD (BioLegend, 640926) was used. Briefly, samples were first stained per normal method with APC anti-CD56 antibody. Cells

(15) The lncRNA SLNCR Recruits the Androgen Receptor to EGR1-Bound Genes in Melanoma and Inhibits Expression of Tumor Suppressor p21Cell reportsMay 21, 2019Schmidt K, Carroll JS, Yee E, Thomas DD, Wert-Lamas L, Neier SC, Sheynkman G, Ritz J, Novina CD.(v2),Illumina,cat# rs-122 Pacific Blue Annexin V Apoptosis Detection Kit with 7-AAD,Biolegend,cat# 640926 Dual-Glo® Luciferase Assay System,Promega,cat# E2920 NE-PER Nuclear and Cytoplasmic Extraction Kit,Thermo Fisher Scientific

(16) The transcription factors TFE3 and TFEB amplify p53 dependent transcriptional programs in response to DNA damageeLifeDecember 6, 2018Jeong E, Brady OA, Martina JA, Pirooznia M, Tunc I, Puertollano R.AB_141633,IF (1:5000) Commercial assay or kit,Pacific Blue AnnexinV apoptosis detection kit,BioLegend,Cat. #: 640926, Chemical compound or drug,Etoposide,Cell Signaling Technology,Cat. #: 2200, Chemical compound or

(17) Combination with SGT-53 overcomes tumor resistance to a checkpoint inhibitorOncoimmunologyJanuary 1, 2018Kim SS, Harford JB, Moghe M, Rait A, Chang EH.level of apoptosis, cells were stained with Annexin V apoptosis detection kit with 7-AAD (BioLegend, 640926). Cells were analyzed by LSRFortessa flow cytometer (BD Biosciences). Ihc Mice were euthanized after

(18) Galectin-8 as an immunosuppressor in experimental autoimmune encephalomyelitis and a target of human early prognostic antibodies in multiple sclerosisPloS oneJanuary 1, 2017Pardo E, Cárcamo C, Uribe-San Martín R, Ciampi E, Segovia-Miranda F, Curkovic-Peña C, Montecino F, Holmes C, Tichauer JE, Acuña E, et al.apoptosis/necrosis was determined with a commercial kit (Pacific Blue TM Annexin V Apoptopsis Detection Kit with 7-AAD; 640926, Biolegend) analyzing death cells by FACS, in the CD4+ GFP+ gated population corresponding to 70% of

(19) SBDS-Deficient Cells Have an Altered Homeostatic Equilibrium due to Translational Inefficiency Which Explains their Reduced Fitness and Provides a Logical Framework for InterventionPLoS geneticsJanuary 1, 2017Calamita P, Miluzio A, Russo A, Pesce E, Ricciardi S, Khanim F, Cheroni C, Alfieri R, Mancino M, Gorrini C, et al.cross-linker (9999 μJ/cm2, three times) and cell death was detected by using the 7AAD-Annexin V kit (640926, BioLegend). For ROS measurement, cells were incubated with 50 μM H2DCF-DA (2’, 7’, -dichlorodihydrofluorescein

(20) TGF-β1 promotes acinar to ductal metaplasia of human pancreatic acinar cellsScientific reportsAugust 3, 2016Liu J, Akanuma N, Liu C, Naji A, Halff GA, Washburn WK, Sun L, Wang P.

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Pacific Blue(TM) Annexin V Apoptosis Detection Kit with 7-AAD from BioLegend

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Pacific Blue(TM) Annexin V Apoptosis Detection Kit with 7-AAD from BioLegend

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