Fig 1: The analysis of highly up-regulated genes in U87-MG cells vs HEB cells. A, A scatter plot representation of RNA-seq expression data. Every point represents a single gene plotted according to the mean log2 ratio between U87-MG cells and HEB cells (x-axis) and the mean U87-MG expression (y-axis). These genes were further sorted according to their expression in U87-MG cells into high (=1000), intermediate (<1000 and =10) and low (<10) expression categories. B, The levels of IL1B, CSF3 and TIMP1 in the serum of GBM patients were significantly higher than those of control patients. *P < 0.05, **P < 0.01. C, VIM protein expression level was higher in U87-MG cells than in HEB cells and GBM tissues showed higher level of VIM than normal brain tissues. NT, normal tissue. Bars = 50 µm. D, Protein interaction of genes distributed into high category from protein interaction databases. E, Enriched signal pathway of GBM high expression genes and their interation genes
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