Description
The Resazurin Cell Viability Kit detects resorufin produced from resazurin conversion by metabolic enzymes in live cells. This kit is expected to work in most cell lines. For most experiments, 0.02-2x105 cells/well should be sufficient, but this can vary depending on the cell type and incubation time. For best results, a cell number titration and incubation time course (as shown in Figure 1) is recommended.
Note: Microbial contaminants will reduce resazurin to resorufin, yielding false positive results. Autofluorescent compounds may interfere with this assay