FITC Annexin V Apoptosis Detection Kit with 7-AAD 640922 from BioLegend

Product Specs
ItemFITC Annexin V Apoptosis Detection Kit with 7-AAD
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number640922
Quantity100 tests
ApplicationsFlow Cytometry (FC)
ReactivityHuman, Mouse, Rat
NCBI Full Gene Nameannexin A5
NCBI Gene AliasesANX5, CPB-I, ENX2, HEL-S-7, PP4, RPRGL3, VAC-alph
Antibody TypePrimary
Conjugate/Tag/LabelFITC
Detection TargetAnnexin V

Add to Compare List

BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Extracellular vesicles recovered from plasma of severe dengue patients induce CD4+ T cell suppression through PD-L1/PD-1 interactionmBioNovember 20, 2023Sharda Kumari, Bhaswati Bandyopadhyay, Anamika Singh, Suruchi Aggarwal, Amit Kumar Yadav, Naval Kishore Vikram, Prasenjit Guchhait, Arup Banerjeeannexin binding buffer, and annexin-FITC, 7-AAD was added as per manufacturers guidelines (catalog no. 640922, BioLegend). Flow cytometry recorded 50k cell events. CFSE labeling of EVs EVs from different groups

(2) Anaplastic thyroid cancer cells reduce CD71 levels to increase iron overload toleranceJournal of Translational MedicineNovember 3, 2023Simona D’Aprile, Simona Denaro, Anna Maria Pavone, Sebastiano Giallongo, Cesarina Giallongo, Alfio Distefano, Lucia Salvatorelli, Filippo Torrisi, Raffaella Giuffrida, Stefano Forte, et al.Annexin V-FITC and propidium iodide (PI) was performed using commercially available assay kit (Cat#640922, Biolegend). Briefly, reagents were added to cell suspension and mixed gently as per manufactures’ instructions.

(3) Th1 cytokines in pediatric acute lymphoblastic leukemiaCancer Immunology, ImmunotherapyNovember 1, 2023Sarah Schober, Jennifer M. Rottenberger, Johannes Hilz, Evi Schmid, Martin Ebinger, Tobias Feuchtinger, Rupert Handgretinger, Peter Lang, Manon Queudeville100%. Annexin V/7-AAD assay We used the FITC Annexin V Apoptosis Detection Kit with 7-AAD by Biolegend (Cat. 640922) according to manufacturer’s instructions. Staurosporine was used as a positive control. Caspase assay The

(4) Irreversible HER2 inhibitors overcome resistance to the RSL3 ferroptosis inducer in non-HER2 amplified luminal breast cancerCell Death DisAugust 18, 2023Soon Young Park, Kang Jin Jeong, Alfonso Poire, Dong Zhang, Yiu Huen Tsang, Aurora S. Blucher, Gordon B. Millsstaining, cells were collected with trypsinization, and washed with PBS, stained with 7AAD (Biolegend, 640922), and analyzed on a Fortessa Flow Cytometry. Results are from three independent experiments performed. MitoSOX

(5) Rewiring cancer drivers to activate apoptosisNatureAugust 10, 2023Sai Gourisankar, Andrey Krokhotin, Wenzhi Ji, Xiaofan Liu, Chiung-Ying Chang, Samuel H. Kim, Zhengnian Li, Wendy Wenderski, Juste M. Simanauskaite, Haopeng Yang, et al.were harvested on ice and washed twice in 2.5% FBS/PBS. Of 7-AAD, 2.5 μl and 2.5 μl of FITC-annexin V (640922, BioLegend) were added. Cells were incubated for 15 min at room temperature, then immediately measured

(6) The miR-23-27-24 clusters drive lipid-associated macrophage proliferation in obese adipose tissueCell ReportsAugust 4, 2023Neil T. Sprenkle, Nathan C. Winn, Kaitlyn E. Bunn, Yang Zhao, Deborah J. Park, Brenna G. Giese, John J. Karijolich, K. Mark Ansel, C. Henrique Serezani, Alyssa H. Hasty, et al.Kits,Thermo Fisher Scientific,Cat# E13183 FITC Annexin V Apoptosis Detection Kit with 7-AAD,BioLegend,Cat# 640922 Foxp3/Transcription Factor Staining Buffer Set,Thermo Fisher Scientific,Cat# 00-5523-00 PE Mouse Anti-Ki-67 Set

(7) SAM-Competitive EZH2-Inhibitors Induce Platinum Resistance by EZH2-Independent Induction of ABC-TransportersCancersJune 3, 2023Groß E, Hilger RA, Schümann FL, Bauer M, Bouska A, Rohde C, Willscher E, Lützkendorf J, Müller LP, Edemir B, et al.The pellets were washed with PBS and stained using FITC Annexin V Apoptosis Detection Kit with 7-AAD (640922; Biolegend, San Diego, CA, USA) according to the manufacturer’s instructions. Cells were analyzed by

(8) Galectin-3 expression in donor T cells reduces GvHD severity and lethality after allogeneic hematopoietic cell transplantationCell ReportsMarch 28, 2023Hemn Mohammadpour, Takemasa Tsuji, Cameron R. MacDonald, Joseph L. Sarow, Hanna Rosenheck, Saeed Daneshmandi, Jee Eun Choi, Jingxin Qiu, Junko Matsuzaki, Agnieszka K. Witkiewicz, et al.isolation Kit II,Miltenyi Biotec,Cat# 130-095-130 FITC-Annexin V Apoptosis Detection Kit,BioLegend™,Cat# 640922 CaspGLOW™ Fluorescein Active Caspase-8 Staining Kit,Invitrogen™,Cat# 88-7005-42 BD Fixation/Permeabilization

(9) Butein Inhibits Cell Growth by Blocking the IL-6/IL-6Rα Interaction in Human Ovarian Cancer and by Regulation of the IL-6/STAT3/FoxO3a PathwayInternational Journal of Molecular SciencesMarch 23, 2023Sun-Ae Park, Young Ju Seo, Lee Kyung Kim, Hee Jung Kim, Kee Dong Yoon, Tae-Hwe Heo(PI). Cell death was analyzed using the FITC Annexin V Apoptosis Detection kit with 7-AAD (Cat.no. 640922, BioLegend, San Diego, CA, USA). Cells were sorted using a FACS Canto II (BD Biosciences, Franklin Lakes,

(10) Differential Lipid Accumulation on HepG2 Cells Triggered by Palmitic and Linoleic Fatty Acids ExposureMoleculesMarch 4, 2023Francisca S. Teixeira, Lígia L. Pimentel, Susana S. M. P. Vidigal, João Azevedo-Silva, Manuela E. Pintado, Luís M. Rodríguez-Alcaláand PA) were determined using the Biolegend FITC Annexin V Apoptosis Detection Kit with 7-AAD Cat. 640922 (San Diego, CA, USA), according to the manufacturer’s instructions and analyzed by flow cytometry using

(11) Whole-mount immunofluorescence imaging and isolation of mesothelium-bound immune cell aggregates during mouse peritoneal inflammationSTAR ProtocolsMarch 1, 2023Margarita Ferriz, Adrián Vega-Pérez, Alejandra Gutiérrez-González, Natalia Alvarez-Ladrón, Carlos ArdavínSet,Thermo Fisher,Cat # 00-5523-00 FITC Annexin V Apoptosis Detection Kit with 7-AAD,BioLegend,Cat # 640922 ,, Experimental models: Organisms/strains,Experimental models: Organisms/strains,Experimental models:

(12) A Novel Combination of Sotorasib and Metformin Enhances Cytotoxicity and Apoptosis in KRAS-Mutated Non-Small Cell Lung Cancer Cell Lines through MAPK and P70S6K InhibitionInternational Journal of Molecular SciencesFebruary 22, 2023Pedro Barrios-Bernal, José Lucio-Lozada, Maritza Ramos-Ramírez, Norma Hernández-Pedro, Oscar Arrietathey were marked with FITC Annexin V Apoptosis Detection Kit with 7-AAD (Biolegend, San Diego, CA, USA 640922). Finally, the cells were evaluated through flow cytometry in accordance with manufacturer’s instructions. 4.5.

(13) Cytochalasin B Influences Cytoskeletal Organization and Osteogenic Potential of Human Wharton’s Jelly Mesenchymal Stem CellsPharmaceuticals (Basel)February 14, 2023Luca Pampanella, Provvidenza Maria Abruzzo, Riccardo Tassinari, Andrea Alessandrini, Giovannamaria Petrocelli, Gregorio Ragazzini, Claudia Cavallini, Valeria Pizzuti, Nicoletta Collura, Silvia Canaider, et al.and 3 μM). The commercial FITC Annexin V Apoptosis Detection Kit with 7-Aminoactinomycin D (7-AAD) (640922; Biolegend, San Diego, CA, USA) was used to evaluate viability and cell death of hWJ-MSCs after CB-treatment

(14) Regulatory T cells suppress the formation of potent KLRK1 and IL-7R expressing effector CD8 T cells by limiting IL-2eLifeJanuary 27, 2023Oksana Tsyklauri, Tereza Chadimova, Veronika Niederlova, Jirina Kovarova, Juraj Michalik, Iva Malatova, Sarka Janusova, Olha Ivashchenko, Helene Rossez, Ales Drobek, et al.33258 (#H3569) dyes were purchased from Invitrogen. FITC Annexin V Apoptosis Detection Kit with 7-AAD (#640922) was purchased from BioLegend. IL-2/S4B6 and IL-2/JES6 complexes IL-2/S4B6 and IL-2/JES6 immunocomplexes

(15) Redox-Dependent Modulation of Human Liver Progenitor Cell Line FateInternational Journal of Molecular SciencesJanuary 18, 2023Francesco Bellanti, Domenica Mangieri, Giorgia di Bello, Aurelio Lo Buglio, Giuseppe Pannone, Maria Carmela Pedicillo, Alberto Fersini, Michał Dobrakowski, Aleksandra Kasperczyk, Sławomir Kasperczyk, et al.sample)/7-amino-actinomycin D (7-AAD, 5 µL for one sample) (FITC Annexin V Apoptosis Detection Kit with 7-AAD, 640922, BioLegend, San Diego, CA, USA) for 30 min at room temperature, and then 500 µL of binding buffer was

(16) Anti-phospholipase A2 receptor antibodies directly induced podocyte damage in vitroRenal FailureDecember 31, 2022Yanfen Li, Juntao Yu, Miao Wang, Zhao Cui, Ming-hui Zhaoantibodies. Apoptosis detection assay The commercial FITC Annexin V Apoptosis Detection Kit with 7-AAD (640922, Biolegend) was used to evaluate the apoptosis of podocytes. After the above treatments, the podocytes

(17) A genetically engineered, stem-cell-derived cellular vaccineCell reports. MedicineDecember 20, 2022Cooper A, Sidaway A, Chandrashekar A, Latta E, Chakraborty K, Yu J, McMahan K, Giffin V, Manickam C, Kroll K, et al.600882 Covid-19 S-protein ELISA kit,Abcam,Cat# ab284402 FITC Annexin V Apoptosis Kit with 7-AAD,Biolegend,Cat# 640922 Cell Trace Yellow,Molecular Probes,Cat# CD3 cell depletion kit,Miltenyi Biotech,Cat# 130-050-101 Primary

(18) Extracellular Vesicles from Pancreatic Cancer Stem Cells Lead an Intratumor Communication Network (EVNet) to fuel tumour progressionGutOctober 1, 2022Carolina F. Ruivo et al.viability/apoptosis was assessed with the FITC annexin V and 7-AAD apoptosis detection kit (BioLegend Cat# 640922), according to manual instructions. As positive control, viable cells (not exposed to gemcitabine or

(19) A proliferative to invasive switch is mediated by srGAP1 downregulation through the activation of TGF-β2 signalingCell reportsSeptember 20, 2022Mondal C, Gacha-Garay MJ, Larkin KA, Adikes RC, Di Martino JS, Chien CC, Fraser M, Eni-Aganga I, Agullo-Pascual E, Cialowicz K, et al.assays,Critical commercial assays ,, FITC Annexin V Apoptosis Detection Kit with 7-AAD,BioLegend,640922 ,, Deposited data,Deposited data,Deposited data ,, RNAseq,Repository,GEO (GSE210488) Proteomics,Repository,PRIDE

(20) Therapeutic Targeting of Macrophage Plasticity Remodels the Tumor-Immune MicroenvironmentCancer ResearchJuly 18, 2022Hee-Jin Jang, Hyun-Sung Lee, Wendong Yu, Maheshwari Ramineni, Cynthia Y. Truong, Daniela Ramos, Taylor Splawn, Jong Min Choi, Sung Yun Jung, Ju-Seog Lee, et al.purchased from Biolegend (352906). Cell viability assay was performing with Annexin V (Biolegend, Cat. No 640922) and DAPI (Life Technologies, Cat. No D1306) staining. Flow cytometry was performed by an LSR Fortessa

Be the First to Write a Review!

FITC Annexin V Apoptosis Detection Kit with 7-AAD from BioLegend

Description

BioLegend

Citations (98)

Reviews

FITC Annexin V Apoptosis Detection Kit with 7-AAD from BioLegend

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map