cAMP Assay Kit (Competitive ELISA) ab133051 from Abcam

Product Specs
ItemcAMP Assay Kit (Competitive ELISA)
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab133051
Quantity1 96TESTS
ReactivityMammals, Mammals, Other species
TypeCompetitive
ApplicationsELISA
Detection TargetcAMP

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Astrocyte and L-lactate in the anterior cingulate cortex modulate schema memory and neuronal mitochondrial biogenesiseLifeNovember 14, 2023Mastura Akter, Mahadi Hasan, Aruna Surendran Ramkrishnan, Zafar Iqbal, Xianlin Zheng, Zhongqi Fu, Zhuogui Lei, Anwarul Karim, Ying Licollected from the ACC were kept at –80°C until further use. cAMP complete ELISA kit (Abcam, USA, Cat #: ab133051) was used to determine the cAMP concentration in ACC dialysate according to the manufacturer’s protocol.

(2) SARS‐CoV‐2 infection activates CREB/CBP in cellular cyclic AMP‐dependent pathwaysJournal of Medical VirologyJanuary 1, 2023Qi Yang, Jielin Tang, Juan Cao, Fengjiang Liu, Muqing Fu, Bao Xue, Anqi Zhou, Sijie Chen, Junjun Liu, Yuan Zhou, et al.MOI of 0.01 for the indicated time point. cAMP content was measured by a commercially available kit (ab133051; Abcam). Data represent mean ± SD of two independent experiments performed in duplicate. Data were fit

(3) BYHW Decoction Improves Cognitive Impairments in Rats with Cerebral Microinfarcts via Activation of the PKA/CREB PathwayOxidative Medicine and Cellular LongevityDecember 30, 2022Bingjie Xue, Bo Ma, Yaoyao Yao, Aimei Zhao, Ying Gao, Jianxun Liu(Cell Signaling Technology, 42406); ATPlite luminescence ATP detection assay system (PerkinElmer, 6016941); cAMP (Abcam, ab133051); JC-1 dye (Life technologies, T3168); H2DCFDA (Life technologies, C6827); MitoTracker red (Life technologies,

(4) Activation of Piezo1 downregulates renin in juxtaglomerular cells and contributes to blood pressure homeostasisCell & bioscienceDecember 5, 2022Yang X, Zeng H, Wang L, Luo S, Zhou Y.To determine cAMP concentrations, treated cells were lysed with 0.1 M HCl and analyzed with a kit (#ab133051, Abcam) following the manufacturer’s instructions. Statistics analysis All experiments were conducted

(5) An optogenetic approach for regulating human parathyroid hormone secretionNature CommunicationsDecember 1, 2022Yunhui Liu, Lu Zhang, Nan Hu, Jie Shao, Dazhi Yang, Changshun Ruan, Shishu Huang, Liping Wang, William W. Lu, Xinzhou Zhang, et al.Human Protein Kinase A ELISA Kit (MBS034086, MyBioSource, CA) and Human Cyclic AMP Complete ELISA (ab133051, Abcam, MA). All experiments are performed according manufacturer instructions. Patch-clamp electrophysiology Whole-cell

(6) A novel prostaglandin I2 agonist, ONO-1301, attenuates liver inflammation and suppresses fibrosis in non-alcoholic steatohepatitis model miceInflammation and RegenerationDecember 1, 2022Satoko Motegi et al.tissue. Measurement of cAMP accumulation cAMP was quantified from liver homogenates using a Direct cAMP ELISA Kit (ab133051; Abcam, Cambridge, UK) according to the manufacturer’s protocol and normalized to total liver weight.

(7) GLP1R inhibits the progression of endometrial carcinoma through activation of cAMP/PKA pathwayJournal of clinical laboratory analysisOctober 1, 2022Li W, Gu Y, Liu S, Ruan F, Lv W.Enzyme‐linked immunosorbent assay (ELISA) The content of cAMP in Ishikawa and RL95‐2 cells was assessed using the cAMP ELISA Kit (ab133051, Abcam) according to the specification of manufacturer. Briefly, the transfected EC cells were lysed

(8) MAGED2 Is Required under Hypoxia for cAMP Signaling by Inhibiting MDM2-Dependent Endocytosis of G-Alpha-SCellsAugust 16, 2022Seaayfan E, Nasrah S, Quell L, Kleim M, Weber S, Meyer H, Laghmani K, Kömhoff M.Measurement Cyclic AMP was measured using a cAMP complete in vitro ELISA kit (Abcam, Berlin, Germany, ab133051), in which a goat anti-rabbit immunoglobulin G binds with a cAMP antibody. Cyclic AMP then binds to

(9) Comparative Evaluation of the Effects of Legacy and New Generation Perfluoralkyl Substances (PFAS) on Thyroid Cells In VitroFront Endocrinol (Lausanne)June 23, 2022Luca De Toni, Andrea Di Nisio, Maria Santa Rocca, Federica Pedrucci, Andrea Garolla, Stefano Dall’Acqua, Diego Guidolin, Alberto Ferlin, Carlo ForestaTSH-108H), were purchased by Creative BioMart Inc. (NY, USA). The Cyclic AMP Complete ELISA Kit (cat# ab133051), used for intracellular cAMP quantification, was purchased by Abcam (Cambridge, UK). The non-radioactive

(10) α-Synuclein Impacts on Intrinsic Neuronal Network Activity Through Reduced Levels of Cyclic AMP and Diminished Numbers of Active Presynaptic TerminalsFrontiers in Molecular NeuroscienceMay 3, 2022Kristian Leite, Pretty Garg, F. Paul Spitzner, Sofia Guerin Darvas, Mathias Bähr, Viola Priesemann, Sebastian Küglerby the colorimetric cAMP assay kit, a competitive enzyme-linked immunosorbent assay (ELISA; Abcam, ab133051), exactly according to the manufacturer’s protocol. Neurons and astrocytes were fluorescently labelled

(11) Attenuation of hepatic ischemia‑reperfusion injury by adipose stem cell‑derived exosome treatment via ERK1/2 and GSK‑3β signaling pathwaysInternational journal of molecular medicineFebruary 1, 2022Zhang Y, Li Y, Wang Q, Zheng D, Feng X, Zhao W, Cai L, Zhang Q, Xu H, Fu H.assay levels The amount of cAMP in serum was quantified using cAMP Complete ELISA kit (product code ab133051; Abcam). Furthermore, prostaglandin E2 (PGE2) was also quantified in serum using PGE2 ELISA kit (product

(12) Salmonella Typhimurium reprograms macrophage metabolism via T3SS effector SopE2 to promote intracellular replication and virulenceNature CommunicationsDecember 1, 2021Lingyan Jiang et al.centrifugation. The cAMP concentration in intracellular bacteria was measured by a Direct cAMP ELISA Kit (Abcam #ab133051) following the manufacturer protocol. Cell lysates were also plated, and cAMP concentrations were normalized

(13) Amelioration of alcohol‑induced gastric mucosa damage by oral administration of food‑polydeoxyribonucleotidesMolecular Medicine ReportsSeptember 9, 2021Jonghwan Kim, Soyoung Chun, Seul-Ong Ohk, Sanghoon Kim, Juwan Kim, Sungoh Lee, Hangyu Kim, Sujong KimMPO activity were analysed. Histamine (cat. no. ab213975), MPO (cat. no. ab155458), cAMP (cat. no. ab133051) and prostaglandin E2 (cat. no. ab133021) ELISA kits (Abcam) were used to measure histamine, MPO, cAMP

(14) Aquaporins Are Essential to Maintain Motility and Membrane Lipid Architecture During Mammalian Sperm CapacitationFrontiers in Cell and Developmental BiologySeptember 1, 2021Ariadna Delgado-Bermúdez, Sandra Recuero, Marc Llavanera, Yentel Mateo-Otero, Andra Sandu, Isabel Barranco, Jordi Ribas-Maynou, Marc YesteU/mL. Concerning cAMP concentration, sperm samples were measured using a colorimetric competitive ELISA kit (ab133051; Abcam, Cambridge, United Kingdom) following the manufacturer’s instructions. Briefly, samples were

(15) Abiraterone Acetate Induces CREB1 Phosphorylation and Enhances the Function of the CBP-p300 Complex, Leading to Resistance in Prostate Cancer CellsClinical Cancer ResearchApril 1, 2021Wenting Pan, Zhouwei Zhang, Hannah Kimball, Fangfang Qu, Kyler Berlind, Konrad H. Stopsack, Gwo-Shu Mary Lee, Toni K. Choueiri, Philip W. Kantoff(cAMP) measurement The concentration of cAMP was determined using a complete ELISA kit (Abcam, Cat. Ab133051) according to the manufacturer’s instruction. Briefly, cells were extracted in 0.1 M HCl and after neutralization,

(16) EP4 activation ameliorates liver ischemia/reperfusion injury via ERK1/2‑GSK3β‑dependent MPTP inhibitionInternational Journal of Molecular MedicineMarch 17, 2020Lin‑Lin Cai, Hai‑Tao Xu, Qi‑Long Wang, Ya‑Qing Zhang, Wei Chen, Dong‑Yu Zheng, Fang Liu, Hong‑Bin Yuan, Yong‑Hua Li, Hai‑Long Fulevel assay Liver tissue were collected at 6 h of reperfusion. A cAMP complete ELISA kit (cat. no. ab133051; Abcam) was used to determine the cAMP levels in liver tissue samples, according to the manufacturer's

(17) Bilateral Ovariectomy Decreases the Levels of Cyclic Nucleotides and Nuclear Phosphorylated Estrogen Receptor-Alpha in Memory-Sensitive Rat Brain RegionsIndian Journal of Pharmaceutical Education and ResearchDecember 31, 2019Ahsas Goyal, Debapriya Garabadulevels Intracellular levels of cyclic nucleotides (cAMP and cGMP) in tissue were determined using direct cAMP (ab133051; Abcam Plc., Cambridge, USA) and cGMP (ab133052; Abcam Plc., Cambridge, USA) enzyme immunoassay kit

(18) Male-Specific cAMP Signaling in the Hippocampus Controls Spatial Memory Deficits in a Mouse Model of Autism and Intellectual DisabilityBiological PsychiatryMay 1, 2019Marta Zamarbide, Adele Mossa, Pablo Muñoz-Llancao, Molly K. Wilkinson, Heather L. Pond, Adam W. Oaks, M. Chiara ManziniLincoln NE). Cyclic AMP (cAMP) levels were measured using the Cyclic AMP Complete ELISA Kit (AbCam, ab133051, Cambridge, MA) following instructions from the manufacturer. Briefly, flash frozen hippocampal or cortical

(19) Biliary Atresia Relevant Human Induced Pluripotent Stem Cells Recapitulate Key Disease Features in a DishJournal of pediatric gastroenterology and nutritionJanuary 1, 2019Tian L, Ye Z, Kafka K, Stewart D, Anders R, Schwarz KB, Jang YY.media for 20 minutes. The concentration of cAMP was determined using a complete ELISA kit (Abcam, Cat. Ab133051) according to the manufacturer's instruction. Statistical Analysis Data is expressed as mean ± standard

(20) Identification of a Genomic Region between SLC29A1 and HSP90AB1 Associated with Risk of Bevacizumab-Induced Hypertension: CALGB 80405 (Alliance)Clinical Cancer ResearchOctober 1, 2018Megan Li et al.cyclic AMP and nitric oxide Intracellular cyclic AMP levels were measured with a Human cAMP ELISA kit (ab133051; Abcam) following sample acetylation. Total nitrate and nitrite levels, as a measure of nitric oxide,

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cAMP Assay Kit (Competitive ELISA) from Abcam

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cAMP Assay Kit (Competitive ELISA) from Abcam

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