Prostaglandin E2 ELISA Kit ab133021 from Abcam

Product Specs
ItemProstaglandin E2 ELISA Kit
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab133021
Quantity1 96TESTS
ReactivitySpecies independent
ApplicationsELISA
Gene NamePtger2
TypeCompetitive
Detection Range39.1 - 2500 pg/ml
Sensitivity13.4 pg/ml
NCBI Gene AliasesEP2
Detection TargetPGE2

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Abcam

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+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) ABHD6 and MAGL control 2-AG levels in the PAG and allodynia in a CSD-induced periorbital model of headacheFrontiers in Pain ResearchMay 23, 2023Erika Liktor-Busa, Aidan A. Levine, Seph M. Palomino, Simar Singh, Jared Wahl, Todd W. Vanderah, Nephi Stella, Tally M. Largent-Milnes(5,000 g, 10 min, 4°C). 5 µl of the supernatant was applied in the immunoassay. PGE2 ELISA kit (Abcam, ab133021) was used according to manufacturer`s guidance. Tissue samples (n = 3–4/group) were weighed and homogenized

(2) Tissue-Oxygen-Adaptation of Bone Marrow-Derived Mesenchymal Stromal Cells Enhances Their Immunomodulatory and Pro-Angiogenic Capacity, Resulting in Accelerated Healing of Chemical BurnsInternational Journal of Molecular SciencesFebruary 17, 2023Marina V. Volkova, Ningfei Shen, Anna Polyanskaya, Xiaoli Qi, Valery V. Boyarintsev, Elena V. Kovaleva, Alexander V. Trofimenko, Gleb I. Filkov, Alexandre V. Mezentsev, Sergey P. Rybalkin, et al.Immunofluorescent Analysis The amount of PGE2 in 5% and 21% oxygen MSC lysates was determined using Prostaglandin E2 ELISA Kit (ab133021, Abcam, Cambridge, UK) according to the manufacturer’s instructions. The obtained values were normalized

(3) Gut microbiota regulate Alzheimer’s disease pathologies and cognitive disorders via PUFA-associated neuroinflammationGutNovember 1, 2022Chun Chen, Jianming Liao, Yiyuan Xia, Xia Liu, Rheinallt Jones, John Haran, Beth McCormick, Timothy Robert Sampson, Ashfaqul Alam, Keqiang Ye88-7324-88 IL-1 beta Mouse Uncoated ELISA Kit,Invitrogen,Cat# 88-7013-22 PGE2 ELISA Kit,Abcam,Cat# ab133021 LTB4 ELISA Kit,Invitrogen,Cat# EHLTB4 Deposited Data,Deposited Data,Deposited Data 16 s RNA sequences,PPMS

(4) Human Allogeneic Liver-Derived Progenitor Cells Significantly Improve NAFLD Activity Score and Fibrosis in Late-Stage NASH Animal ModelCellsSeptember 13, 2022Najimi M, Michel S, Binda MM, Gellynck K, Belmonte N, Mazza G, Gordillo N, Vainilovich Y, Sokal E.from the recovered CMs using specific assays according to the manufacturers’ instructions (PGE2—Ref: ab133021 from Abcam, Cambridge, UK; Kynurenine—Ref: K972-100 from Biovision, Waltham, MA, USA, IFN-γ—Ref: DY258B

(5) A New EGFR Inhibitor from Ficus benghalensis Exerted Potential Anti-Inflammatory Activity via Akt/PI3K Pathway InhibitionCurrent Issues in Molecular BiologyJuly 2, 2022Rania Alaaeldin, Heba Ali Hassan, Islam M. Abdel-Rahman, Reham H. Mohyeldin, Nancy Youssef, Ahmed E. Allam, Sayed F. Abdelwahab, Qing-Li Zhao, Moustafa FathyUSA) was used to measure Mouse inducible nitric oxide synthase (iNOS) activity. A PGE2 ELISA kit (#ab133021, Abcam) was used to measure the PGE2 concentration. A COX-1 ELISA kit (#MBS753621, MyBioSource, Inc.)

(6) Aortic Fibrosis in Insulin-Sensitive Mice with Endothelial Cell-Specific Deletion of Ceacam1 GeneInternational journal of molecular sciencesApril 14, 2022Abu Helal R, Muturi HT, Lee AD, Li W, Ghadieh HE, Najjar SM.endothelin-1 (Endothelin-1 ELISA kit, ab133030, Abcam); prostaglandin E2 (prostaglandin E2 ELISA kit, ab133021, Abcam, Cambridge, UK); PDGF-B (ELISA Kit, MBB00; R&D System, Minneapolis, MN, USA). Hepatic triacylglycerol

(7) Study on the mechanism of Danshen-Guizhi drug pair in the treatment of ovarian cancer based on network pharmacology and in vitro experimentPeerJApril 6, 2022Chongzhen Qin, Menglin Wu, Xinru Wang, Wenda Zhang, Guangzhao Qi, Na-Yi Wu, Xiaoting Liu, Yaoyao Lu, Jingmin Zhang, Yuna Chai0, 20 μm salviolone for 2 h. The cell supernatant was collected and PGE2 was measured by ELISA kit (ab133021; Abcam, Cambridge, UK). The absorbance (OD) of the sample was measured at 415 nm. The average value

(8) Attenuation of hepatic ischemia‑reperfusion injury by adipose stem cell‑derived exosome treatment via ERK1/2 and GSK‑3β signaling pathwaysInternational journal of molecular medicineFebruary 1, 2022Zhang Y, Li Y, Wang Q, Zheng D, Feng X, Zhao W, Cai L, Zhang Q, Xu H, Fu H.Furthermore, prostaglandin E2 (PGE2) was also quantified in serum using PGE2 ELISA kit (product code ab133021; Abcam). Histological analysis Following 2 and 6 h of reperfusion, collected liver tissue samples

(9) Analgesic Mechanisms of Steroid Ointment against Oral Ulcerative Mucositis in a Rat ModelInternational Journal of Molecular SciencesNovember 22, 2021Mako Naniwa et al.Endothelin-1 Assay Kit (17165; Immuno-Biological Laboratories, Fujioka, Japan) and Prostaglandin E2 ELISA Kit (ab133021; Abcam, Cambridge, UK). The assays were carried out in duplicate. The concentrations were standardized

(10) FABP4 knockdown suppresses inflammation, apoptosis and extracellular matrix degradation in IL-1β-induced chondrocytes by activating PPARγ to regulate the NF-κB signaling pathwayMolecular Medicine ReportsOctober 14, 2021Huajie Mao, Bin Han, Hao Li, Yiqing Tao, Weigang Wutriplicate. The level of prostaglandin E2 (PGE2) was determined using a Prostaglandin E2 ELISA kit (cat. no. ab133021; Abcam). Reactive oxygen species (ROS) content in cells was detected by using a ROS/Superoxide Detection Assay kit (Cell-based

(11) Modulation of COX-2 and NADPH oxidase-4 by alpha-lipoic acid ameliorates busulfan-induced pulmonary injury in ratsHeliyonOctober 1, 2021Elhadidy MG, Elmasry A, Elsayed HRH, El-Nablaway M, Hamed S, Elalfy MM, Rabei MR.tissues The levels of PGE2 were measured in the lung tissues by a competitive enzyme immunoassay kit (Abcam, ab133021, Cambridge, UK) following the manufacturer's protocol. The absorbance of PEG2 was measured at 405 nm

(12) Aryl Hydrocarbon Receptor Activation Produces Heat Shock Protein 90 and 70 Overexpression, Prostaglandin E2/Wnt/β-Catenin Signaling Disruption, and Cell Proliferation in MCF-7 and MDA-MB-231 Cells after 24 h and 14 Days of Chlorpyrifos TreatmentChemical Research in ToxicologySeptember 20, 2021Paula Moyano, José Manuel Garcia, Jimena García, Adela Pelayo, Pilar Muñoz-Calero, María Teresa Frejo, Andrea Flores, Javier del Pinountreated control. PGE2 concentration in culture media was analyzed employing a commercial ELISA kit (ab133021; Abcam, Cambridge, U.K.) following the manufacturer’s protocols. Gene expression analysis was developed

(13) Amelioration of alcohol‑induced gastric mucosa damage by oral administration of food‑polydeoxyribonucleotidesMolecular Medicine ReportsSeptember 9, 2021Jonghwan Kim, Soyoung Chun, Seul-Ong Ohk, Sanghoon Kim, Juwan Kim, Sungoh Lee, Hangyu Kim, Sujong Kim(cat. no. ab213975), MPO (cat. no. ab155458), cAMP (cat. no. ab133051) and prostaglandin E2 (cat. no. ab133021) ELISA kits (Abcam) were used to measure histamine, MPO, cAMP and PGE2 levels, respectively, in the

(14) Novel Hydrogen Sulfide (H2S)-Releasing BW-HS-101 and Its Non-H2S Releasing Derivative in Modulation of Microscopic and Molecular Parameters of Gastric Mucosal BarrierInternational Journal of Molecular SciencesMay 14, 2021Dominik Bakalarz et al.concentrations in gastric mucosal samples obtained from the ulcer margin were determined using PGE2 ELISA kit (ab133021, Abcam, Cam- Int. J. Mol. Sci. 2021, 22, 5211 15 of 18 bridge, UK) according to the manufacturer’s

(15) Osteocytic Connexin43 Channels Regulate Bone–Muscle CrosstalkCellsJanuary 26, 2021Guobin Li, Lan Zhang, Kaiting Ning, Baoqiang Yang, Francisca M. Acosta, Peng Shang, Jean X. Jiang, Huiyun Xuin both serum and serum-free primary osteocytes CM were detected using a commercially available kit (ab133021, Abcam, USA), according to the manufacturer’s protocols. 2.11. Statistical Analysis Statistical analysis

(16) LMTK2 regulates inflammation in lipopolysaccharide‑stimulated BV2 cellsExperimental and Therapeutic MedicineJanuary 18, 2021Qianyun Rui, Shugang Cao, Xiaozhu Wang, Xiaoyu Duan, Xinyi Iao, Wanli Dong, Qi Fang, Xueguang Zhang, Qun XueInvitrogen; Thermo Fisher Scientific, Inc.) and prostaglandin E2 (PGE2) levels (Prostaglandin E2 ELISA; ab133021; Abcam). Statistical analysis. GraphPad Prism 8.0 software (GraphPad Software, Inc.) was used for statistical

(17) Cichoric Acid Ameliorates Monosodium Urate-Induced Inflammatory Response by Reducing NLRP3 Inflammasome Activation via Inhibition of NF-kB Signaling PathwayEvidence-Based Complementary and Alternative MedicineJanuary 6, 2021Qi Wang, Bingfeng Lin, Zhifeng Li, Jie Su, Yulin Fenganti-NLRP3 antibody (ab263899), PKA kinase activity assay kit (ab139435), Prostaglandin E2 ELISA Kit (ab133021), and cAMP assay kit (Competitive ELISA) (ab234585) (Abcam, Cambridge, UK); anti-IL-1β antibody (12703),

(18) L-Carnitine potentiates the anti-inflammatory and antinociceptive effects of diclofenac sodium in an experimentally-induced knee osteoarthritis rat modelIran J Basic Med SciSeptember 21, 2020Suzan A. Khodir, Marwa A. Al-Gholam, Heba R. Salemkits. (IL-1β: ab100768, Abcam, Cambridge, UK), (TNF-α: R&D Systems Inc., Minneapolis, USA.), (PG-E2: ab133021, Abcam, Cambridge, UK), and (BALP: MBS703336, MyBioSource, San Diego, USA) according to the manufacturer’s

(19) The protective effect of Bifidobacterium bifidum G9-1 against mucus degradation by Akkermansia muciniphila following small intestine injury caused by a proton pump inhibitor and aspirinGut microbesSeptember 2, 2020Yoshihara T, Oikawa Y, Kato T, Kessoku T, Kobayashi T, Kato S, Misawa N, Ashikari K, Fuyuki A, Ohkubo H, et al.concentrator (Savant, NY, USA). PGE2 quantification was performed using a Prostaglandin E2 ELISA kit (ab133021, Abcam) according to the protocols of the manufacturer. Quantitative RT-PCR RNA extraction was performed

(20) Evidence for Cytoprotective Effect of Carbon Monoxide Donor in the Development of Acute Esophagitis Leading to Acute Esophageal Epithelium LesionsCellsMay 12, 2020Magierowska K, Bakalarz D, Wójcik D, Korbut E, Danielak A, Głowacka U, Pajdo R, Buszewicz G, Ginter G, Surmiak M, et al.same assay microplates. PGE2 concentration in esophageal biopsies was determined using PGE2 ELISA kit (ab133021, Abcam) according to manufacturer’s protocol. Homogenization process of each sample was standardized

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Prostaglandin E2 ELISA Kit from Abcam

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Prostaglandin E2 ELISA Kit from Abcam

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