ApoTox-Glo Triplex Assay G6321 from Promega

Product Specs
ItemApoTox-Glo Triplex Assay
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberG6321
Quantity5 x 10ml
Molecular Weight~81kDa

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Heat stress suppresses MnSOD expression via p53-Sp1 interaction and induces oxidative stress damage in endothelial cells: Protective effects of MitoQ10 and Pifithrin-αHeliyonDecember 1, 2023Jian Gong, Peipei Sun, Li Li, Zhimin Zou, Qihua Wu, Liyun Sun, Hui Li, Zhengtao Gu, Lei Suapoptosis assay Cell viability and apoptosis were assessed using the ApoTox-Glo™ Triplex Assay kit (Promega, G6320) in 96-well cell cultures. Cells were treated with reaction substrate or control carrier (100 μL), incubated

(2) DoxoDB: A Database for the Expression Analysis of Doxorubicin-Induced lncRNA GenesNon-Coding RNAJuly 13, 2023Rebecca Distefano, Mirolyuba Ilieva, Jens Hedelund Madsen, Sarah Rennie, Shizuka Uchidacellular viability, cytotoxicity, and apoptosis, ApoTox-Glo Triplex Assay (Promega, Nacka, Sweden, #G6321) was used according to the manufacturer’s protocol. The fluorescent and luminescent signals were read

(3) Integrated multi-omics for rapid rare disease diagnosis on a national scaleNature MedicineJuly 1, 2023Sebastian Lunke, Sophie E. Bouffler, Chirag V. Patel, Sarah A. Sandaradura, Meredith Wilson, Jason Pinner, Matthew F. Hunter, Christopher P. Barnett, Mathew Wallis, Benjamin Kamien, et al.cytotoxicity were assessed after heat shock, with the ApoTox-Glo Triplex Assay kit (Promega, cat. no. G6320) according to previously described methods with minor modifications22. To expose cells to heat shock

(4) Spatial and temporal intra-tumoral heterogeneity in advanced HGSOC: Implications for surgical and clinical outcomesCell Reports MedicineJune 20, 2023Paula Cunnea, Edward W. Curry, Elizabeth L. Christie, Katherine Nixon, Chun Hei Kwok, Ahwan Pandey, Ratri Wulandari, Kerstin Thol, Jennifer Ploski, Cristina Morera-Albert, et al.assays,Critical commercial assays,Critical commercial assays ,, ApoTox-Glo Triplex Assay,Promega,Cat #G6321 ,, Deposited data,Deposited data,Deposited data ,, Infinium OmniExpress-24 v1.3 BeadChip (SNP genotyping)

(5) Targeting and Sensitization of Breast Cancer Cells to Killing with a Novel Interleukin-13 Receptor α2-Specific Hybrid Cytolytic PeptideCancers (Basel)May 16, 2023Riaz Jannoo, William Walker, Venkateswarlu Kanamarlapudicell viability, cytotoxicity, and apoptosis was determined by ApoTox-GloTM Triplex Assay (Promega (#G6320)): Briefly, 40,000 cells per well were plated into the 96-well µClear® half area black (for the cell

(6) Empagliflozin reduces podocyte lipotoxicity in experimental Alport syndromeeLifeMay 2, 2023Ge M, Molina J, Kim JJ, Mallela SK, Ahmad A, Varona Santos J, Al-Ali H, Mitrofanova A, Sharma K, Fontanesi F, et al.control,Thermo Scientific,Cat# 4390843,20 nM Commercial kit,ApoTox-Glo Triplex assay,Promega,Cat# G6320, Commercial kit,Amplex Red Cholesterol Assay,Thermo Scientific,Cat# A12216, Commercial kit,Triglyceride

(7) Combined inhibition of BADSer99 phosphorylation and PARP ablates models of recurrent ovarian carcinomaCommunications MedicineDecember 1, 2022Xi Zhang, Liqiong Wang, Shu Chen, Peng Huang, Lan Ma, Hui Ding, Basappa Basappa, Tao Zhu, Peter E. Lobie, Vijay Pandeyexpansion. At termination, viability and apoptosis were determined using the ApoTox-Glo Triplex Assay Kit (G6320, Promega, China)49–52. The clinical characteristics, molecular profiling, and culture conditions for

(8) Mono-(2-ethyl-5-hydroxyhexyl) phthalate promotes uterine leiomyoma cell survival through tryptophan-kynurenine-AHR pathway activationProceedings of the National Academy of Sciences of the United States of AmericaNovember 22, 2022Iizuka T, Yin P, Zuberi A, Kujawa S, Coon JS 5th, Björvang RD, Damdimopoulou P, Pacyga DC, Strakovsky RS, Flaws JA, et al.viability, cytotoxicity, and apoptosis of LM and MM cells were evaluated by ApoTox-Glo Triplex Assay (G6320, Promega) following the manufacturer’s protocol. Briefly, 8,000 to 10,000 cells per well were cultured

(9) Effects of Piptoporus betulinus Ethanolic Extract on the Proliferation and Viability of Melanoma Cells and Models of Their Cell MembranesInternational Journal of Molecular SciencesNovember 11, 2022Justyna Bożek, Joanna Tomala, Sylwia Wójcik, Beata Kamińska, Izabella Brand, Ewa Pocheć, Elżbieta Szostakdehydrogenase (LDH) activity test (MAK066, Sigma-Aldrich, St. Louis, MO, USA), as well as ApoTox-Glo assay (G6320, Promega, Madison, WI, USA) were used to analyze both cell viability and apoptosis. Cells, detached

(10) Photobiomodulation at 830 nm Stimulates Migration, Survival and Proliferation of Fibroblast CellsDiabetes, Metabolic Syndrome and Obesity: Targets and TherapySeptember 21, 2022Olajumoke Arinola Oyebode, Nicolette Nadene Houreld(PerkinElmer) in relative light units (RLU). ApoTox-Glo™ Triplex Assay The ApoTox-Glo triplex assay (Promega, G6320) is more complex and is centered on the proteolytic action of biomarkers present in the cell. It was

(11) Aristolochic acid I induces proximal tubule injury through ROS/HMGB1/mt DNA mediated activation of TLRsJournal of cellular and molecular medicineAugust 1, 2022Upadhyay R, Batuman V.triplex assay We purchased the ApoToxGloTM Triplex Assay from Promega (Fitchburg, WI, USA, catalogue G6320) and performed according to the manufacturer's instructions. 2.6 Flow cytometry We used Beckman coulter

(12) Inhibition of BAD-Ser99 phosphorylation synergizes with PARP inhibition to ablate PTEN-deficient endometrial carcinomaCell Death & DiseaseJune 20, 2022Xi Zhang, Peng Huang, Liqiong Wang, Shu Chen, Basappa Basappa, Tao Zhu, Peter E. Lobie, Vijay Pandeyassays, cell viability, apoptosis, and cytotoxicity were performed using ApoTox-Glo™ Triplex Assay Kit (G6320, Promega, China) as previously described [40]. Phosphatidylserine exposure and cell death, Live/Dead

(13) Progestin Significantly Inhibits Carcinogenesis in the Mogp-TAg Transgenic Mouse Model of Fallopian Tube CancerCancer prevention research (Philadelphia, Pa.)February 1, 2022Nelson OL, Rosales R, Turbov JM, Thaete LG, Cline JM, Rodriguez GC.FTE cell viability, cytotoxicity and caspase activity were examined using ApoTox-Glo™ Triplex Assay (#G6321 Promega) according to the manufacturer’s instructions using 5000 cells/well on a 96-well white plate

(14) The endocannabinoidome mediator N-oleoylglycine is a novel protective agent against 1-methyl-4-phenyl-pyridinium-induced neurotoxicityFrontiers in aging neuroscienceJanuary 1, 2022Lauritano A, Cipollone I, Verde R, Kalkan H, Moriello C, Iannotti FA, Di Marzo V, Piscitelli F.and N-Oleoylglycine from Cayman Chemical Company (Ann Arbor, MI, USA); ApoTox-Glo Triplex Assay kit (G6321) from Promega Corporation (Fitchburg, WI, USA); Human interleukin-1 beta (IL-1β) and Human TNF-α ELISA

(15) Integrated analysis of mRNA and miRNA profiles revealed the role of miR-193 and miR-210 as potential regulatory biomarkers in different molecular subtypes of breast cancerBMC CancerDecember 1, 2021Adriane F. Evangelista, Renato J. Oliveira, Viviane A. O. Silva, Rene A. D. C. Vieira, Rui M. Reis, Marcia M. C. Marques

(16) Serum from Patients with Severe Alcoholic Liver Cirrhosis Inhibits Proliferation and Migration of Human Coronary Artery Smooth Muscle CellsJournal of Clinical MedicineNovember 23, 2021Mare Mechelinck, Miriam Peschel, Moriz A. Habigt, Daniela Kroy, Michael Lehrke, Marius J. Helmedag, Rolf Rossaint, Matthias Barton, Marc HeinApoTox-GloTM Triplex Assay, according to the manufacturer’s instructions (Promega GmbH, Madison, WI, USA, G6320). Luminescence was measured to quantify apoptosis. The assay was run in duplicate for each individual

(17) Cognitive Impairment in Frail Hypertensive Elderly Patients: Role of HyperglycemiaCellsAugust 17, 2021Pasquale Mone, Jessica Gambardella, Antonella Pansini, Antonio de Donato, Giuseppe Martinelli, Eugenio Boccalone, Alessandro Matarese, Salvatore Frullone, Gaetano Santulli[60,61]. Cell death was evaluated using a Caspase-Glo® 3/7 assay (Promega, Madison, WI, USA; catalog number: #G6321), as we described [62]. All reagents were from Millipore-Sigma (Burlington, MA, USA), unless otherwise

(18) Synthesis and Pharmacological In Vitro Investigations of Novel Shikonin Derivatives with a Special Focus on Cyclopropane Bearing DerivativesInternational Journal of Molecular SciencesMarch 9, 2021Nadine Kretschmer, Antje Hufner, Christin Durchschein, Katrin Popodi, Beate Rinner, Birgit Lohberger, Rudolf BauerTriplex Assay The ApoToxGloTM Triplex Assay was purchased from Promega (Fitchburg, WI, USA, cat. no. G6320) and performed according to the manufacturer’s instructions. In brief, 10,000 cells/well (WM9 or MUG-Mel2

(19) Hyaluronan-Conjugated Carbon Quantum Dots for Bioimaging UseACS Applied Materials & InterfacesJanuary 13, 2021Bedia Begüm Karakoçak, Amine Laradji, Tina Primeau, Mikhail Y. Berezin, Shunqiang Li, Nathan RaviCulture Collection (ATCC) (Manassas, VA). The apoptosis assessment assay: ApoTox-Glo Triplex assay (cat# G6320) and reactive oxygen species (ROS) detection assay: ROS Glo H2O2 Assay (cat# G8820) were both obtained

(20) A small-molecule allosteric inhibitor of BAX protects against doxorubicin-induced cardiomyopathyNature CancerAugust 11, 2020Dulguun Amgalan et al.treated with 200 nM DOX for 24 h and cell death was assessed using a Caspase-Glo 3/7 assay (Promega, G6320). Toxicity studies in vivo Vehicle or BAI1 at 2 mg kg−1 was administered i.p. (~4 μl g−1) every other

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ApoTox-Glo Triplex Assay

ApoTox-Glo Triplex Assay from Promega

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ApoTox-Glo Triplex Assay from Promega

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