- Human B-cell lymphoma-extra large (Bcl-xl)ELISA Kit
- CUSABIO Technology LLC
- Pricing InfoSupplier PageView Company Product Page
- CSB-EL027199HU
- 96T
- 0.156 ng/mL-10 ng/mL
- 450 nm
- Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
- serum, plasma, cell culture supernates
- 0.039 ng/mL
- 50-100ul
- B-cell lymphoma-extra large (Bcl-xl)ELISA kit
- 1-5h
- ELISA
- ELISA Kit
- Homo sapiens (Human)
- BCL2 associated agonist of cell death
- BBC2, BCL2L8
- Cancer
Specifications/Features
This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Bcl-xl has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Bcl-xl present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Bcl-xl is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Bcl-xl bound in the initial step. The color development is stopped and the intensity of the color is measured.