Fig 1: Trophoblast Hyal-2 overexpression impairs uterine NK cell recruitment and function.(A) Representative images of DBA+ uterine NK cells staining in E6.5 decidua (n = 4 dams in each group). (B) Immunofluorescence of colocalized DBA+ uterine NK cells with hyaluronan receptor RHAMM at E6.5 decidua. (C) Impaired uterine NK cell recruitment demonstrated by decreased IL-15/IL-15R complexes detected by ELISA (n = 5 dams in each group 323.4 ± 23.49 pg/mL [control]; 213.65 ± 30.43 pg/mL [overexpression], P = 0.2). (D) Western blot analysis for NCR-1 in E6.5 decidual extracts demonstrated decreased accumulation of NCR-1–expressing NK cells in Hyal-2 OEx foster dams (n = 3 dams, 2 implantation sites in each group). (E) Flow cytometry analysis of E6.5 implantation sites harvested from foster dams of both groups. Note decreased ratio of CD45+NCR-1+ population in the Hyal-2 OEx group. (F) Immunofluorescence of TNFRSF9 expressed by uterine NK cells, in the mesometrial pole in E6.5 decidua. The statistical analysis applied was Student’s t test (C).