Fig 1: Autophagy and apoptosis in MDA-MB-231 cells. Represented as follows: fold change in protein expression of (a) beclin-1, (b) P62, (c) LC3B-II, (d) cleaved PARP-1, (e) representative western blot analysis, SDS-PAGE of beclin-1, P62, LC3B-II/LC3B-I, cleaved PARP-1 and ß-actin, (f) BCL-2, and (g) cleaved caspase-3 protein levels. Results are expressed as the mean and standard error of the mean (SEM) (n = 3). Columns with dissimilar letters (a, b, c…) overhead within the same histogram are significantly different, and columns with the same letters are not significantly different at p < 0.05. MDA group: untreated MDA-MB-231 cells served as control; MDA + Eb-ZFO group: MDA-MB-231 cells treated with Ebselen (Eb) and ZnFe2O4 nanoparticles (ZFO); MDA + IR group: MDA-MB-231 cells exposed to ?-radiation; and MDA + Eb-ZFO + IR group: MDA-MB-231 cells treated with Eb-ZFO and exposed to IR.
Fig 2: Autophagy and apoptosis in HT-29 cells. Represented as follows: fold change in protein expression of (a) Beclin-1; (b) P62; (c) LC3B-II; (d) cleaved PARP-1; (e) representative western blot analysis, SDS-PAGE of Beclin-1, P62, LC3B-II/LC3B-I, cleaved PARP-1, and ß-actin; (f) BCL-2; and (g) cleavedcaspase-3 protein levels. Results are expressed as the mean and standard error of the mean (SEM) (n = 3). Columns with dissimilar letters (a, b, c…) overhead within the same histogram are significantly different, and columns with the same letters are not significantly different at p < 0.05. HT-29 group: untreated HT-29 cells served as control. HT-29 + Eb-ZCFO group: HT-29 cells treated with Ebselen (Eb) and ZnCe0.06Fe1.94O4 nanoparticles (ZCFO). HT-29 + IR group: HT-29 cells exposed to ?-radiation. HT-29 + Eb-ZCFO + IR group: HT-29 cells treated with Eb-ZCFO and exposed to IR.
Fig 3: Expressions of miR-708, BAMBI, Wnt10B, P53, Bcl-2, VEGF, Fas, Bax, Caspase-3, and cleaved Caspase-3 after transfection in each group. A, Histogram of mRNA expressions. B, Histogram of protein expressions. C, electrophoretograms of protein expressions. *P < .05, compared to the normal group; # P < .05, compared with the blank and NC groups. NC indicates negative control.
Fig 4: Protein levels of apoptosis biomarker. The level of Caspase-3 (A), p53 (B), Bax (C), and Bcl-2 (D) in HepG-2, MCF-7, Bj-1, and MCF-12F treated with or without the IC50 of MP, MP/NabM, and MP/IHM. MP: milk proteins concentrate; MP/NabM: milk proteins/Nabeq mucilage complex; MP/IHM: milk proteins/Isabgol husk mucilage complex. Data are average of triplicates. Measurements with different letters (a, b, c and d) are significantly different (p < 0.05).
Fig 5: Analysis of apoptosis genes transcript in HepG-2, MCF-7, Bj-1, and MCF-12F cells treated with MP, MP/IHM, and MP/NabM. Profiling of mRNA transcript levels of key pro- (Casp3 (A), p53 (B), Bax (C) and anti-apoptotic Bcl-2 (D)). Gene expression levels were quantified after 72 h by RT-qPCR employing 18S as a housekeeping gene for normalization as detailed in the methods. Significant differences between the means of individual treatments and control were analyzed by one-side Student’s t-test. Histograms represent mean expression level as fold change SD for 3 technical and 2 biological replicas with different letters (a, b and c) are significantly different (p-value = 0.05). MP: milk proteins concentrate; MP/NabM: milk proteins/Nabeq mucilage complex; MP/IHM: milk proteins/Isabgol husk mucilage complex.
Supplier Page from Abcam for Human Bcl-2 ELISA Kit