Fig 1: ELISA analysis of serum inflammatory factors. (a) Interleukin- (IL-) 1ß. (b) IL-17A. (c) Interferon- (IFN-) ?. (d) IL-10. Ap < 0.05 vs. the CG group, Bp < 0.05 vs. the MG group, Cp < 0.05 vs. the PG group, Dp < 0.05 vs. the HexG group, Ep < 0.05 vs. the HepG group, and Fp < 0.05 vs. the OG group. n = 8 for each group.
Fig 2: Anti-inflammatory effects of chitosan oligosaccharides (chitobiose, chitotriose, chitotetraose, chitopentaose, chitohexaose, chitoheptaose, and chitooctaose) in LPS inducing the release of inflammatory cytokines in RAW264.7 cells. (a) Interleukin- (IL-) 1ß. (b) IL-10. (c) IL-17A. (d) Interferon- (IFN-) ?. Ap < 0.05 vs. the control, Bp < 0.05 vs. the chitobiose group, Cp < 0.05 vs. the chitotriose group, Dp < 0.05 vs. the chitotetraose group, Ep < 0.05 vs. the chitopentaose group, Fp < 0.05 vs. the chitohexaose group, Gp < 0.05 vs. the chitoheptaose group, and Hp < 0.05 vs. the chitooctaose group.
Fig 3: (A) Schematic diagram showing bilateral PVN microinjection sites and GFP expression in the PVN, and (B) effect of bilateral PVN microinjection of IL-17RA siRNA on mRNA expression of IL-17A receptor IL-17RA in normal rats 1 week after bilateral PVN microinjections of IL-17RA siRNA AAV virus. (C,D) Effect of bilateral PVN microinjection of IL-17RA siRNA on mRNA expression of IL-17A receptors IL-17RA and IL-17RC in the PVN of SHAM and HF rats 4 weeks after CL. 3V: third cerebroventricle; Scr: scrambled siRNA. Values are mean ± SEM (n = 6-8 for each group) and are expressed as a fold change compared to control or SHAM + Scr siRNA; *p < 0.05.
Fig 4: (A) Plasma IL-17A levels in SHAM and HF rats at 1-4 weeks after CL. (B) CSF IL-17A levels in SHAM and HF rats 4 weeks after CL. (C) Correlation between plasma IL-17A levels and CSF IL-17A levels in SHAM and HF rats 4 weeks after CL. CSF: cerebrospinal fluid. Data are mean ± SEM (n = 7-8 for each group); *p < 0.05.
Fig 5: (A) mRNA expression of IL-17A and its receptors IL-17RA and IL-17RC in the PVN of SHAM and HF rats 4 weeks after CL. (B) Representative confocal images showing expression of IL-17RA (green) and NeuN (a neuronal marker, red) and merged images (yellow) in the PVN of SHAM and HF rats 4 weeks after CL. (C) IL-17RA immunofluorescence intensity in 4 subnuclear regions of the PVN including dorsal parvocellular (dp), medial parvocellular (mp), ventrolateral parvocellular (vlp), and posterior magnocellular (pm), in SHAM and HF rats 4 weeks after CL. Values are expressed as means ± SEM (n = 4-8 for each group); *p < 0.05.
Supplier Page from Abcam for Rat IL-17A ELISA Kit