ADP/ATP Ratio Assay Kit (Bioluminescent) ab65313 from Abcam

Product Specs
ItemADP/ATP Ratio Assay Kit (Bioluminescent)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab65313
Quantity200 test
MethodFuncS
Sample TypeTissue, Adherent cells, Suspension cells
Assay Time0h 30m
Sensitivity100 cells/well
ApplicationsCellular Assay
TypeSemi-quantitative
Detection TargetADP/ATP

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Cell-type-specific responses to the microbiota across all tissues of the larval zebrafishCell ReportsFebruary 13, 2023Michelle S. Massaquoi, Garth L. Kong, Daisy Chilin-Fuentes, Julia S. Ngo, Patrick F. Horve, Ellie Melancon, M. Kristina Hamilton, Judith S. Eisen, Karen Guillemin200-1000 assays",Thermo Fisher Scientific,Cat# A22066 ADP/ATP Ratio Assay Kit (Bioluminescent),Abcam,Cat# ab65313 ,, Deposited data,Deposited data,Deposited data ,, Raw FASTQ files,This study,NCBI SRA: PRJNA885906 ,, Experimental models

(2) CoQ Regulates Brown Adipose Tissue Respiration and Uncoupling Protein 1 ExpressionAntioxidants (Basel)December 22, 2022Ching-Fang Chang, Amanda L. Gunawan, Irene Liparulo, Peter-James H. Zushin, Ambre M. Bertholet, Yuriy Kirichok, Andreas StahlReleasing Buffer, and immediately used for measuring ADP/ATP ratio with an ADP/ATP ratio assay kit (ab65313, Abcam, Cambridge, UK). For NAD/NADH ratio, cells were homogenized in NADH/NAD Extraction Buffer and

(3) Overexpression of Rhodopsin or Its Mutants Leads to Energy Metabolism Dysfunction in 661w CellsInvestigative Opthalmology & Visual ScienceDecember 5, 2022Yang Liu, Xin Wang, Ruowen Gong, Gezhi Xu, Min ZhuThermo Fisher Scientific). The ADP/ATP ratio was detected with an ADP/ATP Ratio Assay Kit (Bioluminescent) (ab65313; Abcam). Reaction mix was added to a luminometer plate, and the background signal was read on the Tecan

(4) Altered glycolysis triggers impaired mitochondrial metabolism and mTORC1 activation in diabetic Î²-cellsNat CommunNovember 14, 2022Elizabeth Haythorne, Matthew Lloyd, John Walsby-Tickle, Andrei I. Tarasov, Jonas Sandbrink, Idoia Portillo, Raul Terron Exposito, Gregor Sachse, Malgorzata Cyranka, Maria Rohm, et al.indicated for 1 h. ATP/ADP ratio was measured using a bioluminescent ADP/ATP ratio assay kit (Abcam, ab65313) according to the manufacturer’s instructions. Respirometry The Seahorse XF24 Extracellular Flux Analyser

(5) Vitamin C modulates the levels of several proteins of the mitochondrial complex III and its activity in the mouse liverRedox biologyNovember 1, 2022Aumailley L, Bourassa S, Gotti C, Droit A, Lebel M.GL40 group were used. ATP levels were determined in the whole liver using a luminescent assay kit (#ab65313 from Abcam, Cambridge, MA) following the manufacturer's protocol. Approximately 60 μg of liver tissue

(6) Platelets and the Cybernetic Regulation of Ischemic Inflammatory Responses through PNC Formation Regulated by Extracellular Nucleotide Metabolism and SignalingCellsSeptember 27, 2022Tiago F. Granja, David KÃ¶hler, Veronika Leiss, Claudia Eggstein, Bernd NÃ¼rnberg, Peter Rosenberger, Sandra Beer-Hammerresuspended platelets (1 × 108 platelets/mL) the release of ADP was measured with an ADP/ATP ratio assay kit (ab65313; Abcam, Cambridge, UK). An ADP standard concentration curve (Chrono-Par® from Chrono-Log®, Hamburg,

(7) Metabolic reprogramming from glycolysis to fatty acid uptake and beta-oxidation in platinum-resistant cancer cellsNature communicationsAugust 5, 2022Tan Y, Li J, Zhao G, Huang KC, Cardenas H, Wang Y, Matei D, Cheng JX.and ATP assay NADP/NADPH and ADP/ATP were measured by using commercial kits (Abcam, #ab65349 and #ab65313). For NADPH measurement, cells (~1 × 106 cells) were pelleted and extracted using NADPH/NADP extraction

(8) Differential In Vitro Effects of SGLT2 Inhibitors on Mitochondrial Oxidative Phosphorylation, Glucose Uptake and Cell MetabolismInternational Journal of Molecular SciencesJuly 19, 2022Elmar Zügner et al.for three hours. ADP and ATP levels in the samples were detected using the ADP/ATP Ratio Assay Kit (ab65313, Abcam, Cambridge, UK). Cells were grown as described above. Prior to the measurement, the culture medium

(9) Uncoupling protein 2 and aldolase B impact insulin release by modulating mitochondrial function and Ca(2+) release from the ERiScienceJuly 15, 2022Inoue R, Tsuno T, Togashi Y, Okuyama T, Sato A, Nishiyama K, Kyohara M, Li J, Fukushima S, Kin T, et al.Reverse Transcription Kit,Thermo Fisher Scientific,Cat# 4368813 ADP/ATP Ratio Assay Kit,Abcam,Cat# ab65313 VECTASTAIN® Elite® ABC HRP Kit,Vector Laboratories,Cat# PK-6101 DAB Peroxidase (HRP) Substrate Kit,Vector Laboratories

(10) Myosin Heavy Chain Converter Domain Mutations Drive Early-Stage Changes in Extracellular Matrix Dynamics in Hypertrophic CardiomyopathyFrontiers in Cell and Developmental BiologyJune 16, 2022Jeanne Hsieh, Kelsie L. Becklin, Sophie Givens, Elizabeth R. Komosa, Juan E. Abrahante Lloréns, Forum Kamdar, Branden S. Moriarity, Beau R. Webber, Bhairab N. Singh, Brenda M. Ogleperformed as per manufacturer’s instructions using ADP/ATP Ratio Assay Kit (Bioluminescent) (Abcam, ab65313). Isogenic control and mutant hiPSCs were differentiated to cardiomyocytes as described above and single-cell

(11) Pyruvate-Driven Oxidative Phosphorylation is Downregulated in Sepsis-Induced Cardiomyopathy: A Study of Mitochondrial ProteomeShockApril 1, 2022Briana K. Shimada et al.samples were then run using a commercially available ADP/ATP ratio assay kit from Abcam (Catalog No: ab65313) per manufacturer's instructions. ADP/ATP ratio were calculated as described in the manufacturer's protocol.

(12) AMPK–mTOR–Mediated Activation of Autophagy Promotes Formation of Dormant Polyploid Giant Cancer CellsCancer ResearchMarch 1, 2022Bo You, Tian Xia, Miao Gu, Zhenxin Zhang, Qicheng Zhang, Jianhong Shen, Yue Fan, Hui Yao, Si Pan, Yingna Lu, et al.instructions. Cellular ATP levels were measured using an ADP/ATP ratio assay kit (Bioluminescent; Abcam, ab65313) according to the manufacturer's instructions. Briefly, 100 μL of the reaction mix was added to obtain

(13) Mitochondrial homeostasis regulates definitive endoderm differentiation of human pluripotent stem cellsCell death discoveryFebruary 17, 2022Lv J, Yi Y, Qi Y, Yan C, Jin W, Meng L, Zhang D, Jiang W.determination ATP/ADP ratio was measured using the bioluminescent-based ADP/ATP Ratio Assay Kit (Abcam, Cat# ab65313) according to the manufacturer’s protocol. In brief, the cells of different states or treatments were

(14) Limonin, an AMPK Activator, Inhibits Hepatic Lipid Accumulation in High Fat Diet Fed MiceFrontiers in PharmacologyJanuary 24, 2022Si-wei Wang, Tian Lan, Hang-fei Chen, Hao Sheng, Chun-yi Xu, Li-feng Xu, Fang Zheng, Feng Zhangconcentrations of limonin dissolved in DMSO for 2 h. ADP/ATP ratio was measured by ADP/ATP Ratio Assay Kit (#ab65313, Abcam, Burlingame, CA) according to the manufacturer’s instruction. Plasmids and Transfection AMPK-DN

(15) Changes in the Left Ventricular Eicosanoid Profile in Human Dilated CardiomyopathyFrontiers in cardiovascular medicineJanuary 1, 2022Sosnowski DK, Jamieson KL, Darwesh AM, Zhang H, Keshavarz-Bahaghighat H, Valencia R, Viveiros A, Edin ML, Zeldin DC, Oudit GY, et al.extinction coefficient, sample volume, and protein concentration. A bioluminescent assay kit (Abcam, ab65313) was used to quantify ATP and ADP levels in crude mitochondrial fractions from LV tissues. Samples were

(16) Pharmacological Inhibition of NOX4 Improves Mitochondrial Function and Survival in Human Beta-CellsBiomedicinesDecember 8, 2021Andris Elksnis, Jing Cen, Per Wikström, Per-Ola Carlsson, Nils Welshnm) was quantified by flow cytometry. 2.6. ATP and ADP A bioluminescent ADP/ATP assay kit (Abcam, ab65313) was used to examine changes in ADP and ATP levels in EndoC-βH1 cells following incubation for 20 min

(17) Singular and short-term anesthesia exposure in the developing brain induces persistent neuronal changes consistent with chronic neurodegenerative diseaseScientific ReportsDecember 1, 2021Kaley Hogarth, Ramesh Babu Vanama, Greg Stratmann, Jason T. MaynesAbcam Bioluminescent ADP/ATP Ratio Assay Kit and performed according to manufacturer’s instructions (ab65313, Cambridge, MA, USA). In brief, 100 µL of ATP Reaction Mix (1× ATP Monitoring Enzyme in Nucleotide Releasing

(18) N-acetylcysteine supplementation did not reverse mitochondrial oxidative stress, apoptosis, and inflammation in the salivary glands of hyperglycemic ratsNutrition & DiabetesNovember 9, 2021Zalewska Anna, KuÄ‡ Joanna, ZiÄ™ba Sara, Matczuk Jan, Kostecka-SochoÅ„ Paula, Szarmach Izabela, Åadny Jerzy Robert, Å»endzian-Piotrowska MaÅ‚gorzata, Maciejczyk MateuszATP to ADP by luciferase. The procedure was performed using a commercial kit (ADP/ATP Ratio Assay Kit ab65313; Abcam, USA) according to the manufacturer’s instructions. Mitochondrial hydrogen peroxide (H2O2) was

(19) Nitro-Deficient Niclosamide Confers Reduced Genotoxicity and Retains Mitochondrial Uncoupling Activity for Cancer TherapyInternational journal of molecular sciencesSeptember 27, 2021Ngai TW, Elfar GA, Yeo P, Phua N, Hor JH, Chen S, Ho YS, Cheok CF.h. Cells were then harvested via trypsinisation and lysed in nucleotide releasing buffer (Abcam, USA ab65313). ATP levels were obtained using ADP/ATP Ratio Bioluminescent Assay Kit (Abcam, USA ab65313). Luminescence

(20) Mesenchymal stromal cell aging impairs the self-organizing capacity of lung alveolar epithelial stem cellseLifeSeptember 16, 2021Chanda D, Rehan M, Smith SR, Dsouza KG, Wang Y, Bernard K, Kurundkar D, Memula V, Kojima K, Mobley JA, et al.kit,Mouse XL Cytokine Array Kit,R&D Systems,ARY028, Commercial assay or kit,ADP/ATP Ratio Kit,Abcam,Ab65313, Commercial assay or kit,Novex Colloidal Blue Staining kit,Invitrogen,LC6025, "Strain, strain background

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ADP/ATP Ratio Assay Kit (Bioluminescent) from Abcam

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ADP/ATP Ratio Assay Kit (Bioluminescent) from Abcam

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