Fig 1: Glomerular endothelial activation in MCD and SSNS. (a) Glomerular transcriptome analysis from patients with MCD in relapse versus controls obtained from Nephroseq.org (“Ju CKD Glom cohort”); (b) immunofluorescence of human kidney showed up-regulation of caveolin-1 (green), colocalizing with rhodamine lectin (red), in adults with MCD in relapse compared with controls (n = 11 vs. n = 5), scale bars = 100 µm. Right graph represents immunofluorescence analysis of caveolin-1 expression in human kidney tissue, P = 0.0005; (c) Western blots of THBD and GAPDH from hiGEnC exposed for 24 hours to 10% sera from children with MCD/SSNS in relapse (n = 1/n = 4, respectively) versus control subjects (n = 5). Right graph represents densitometry analysis of Western blots from hiGEnC lysates, P = 0.01. Data presented as median ± interquartile range. GAPDH, Glyceraldehyde 3-phosphate dehydrogenase; hiGEnC, human immortalized glomerular endothelial cell; MCD, minimal change disease; SSNS, steroid sensitive nephrotic syndrome; THBD, thrombomodulin.