Fluo-8 Calcium Flux Assay Kit - No Wash ab112129 from Abcam

Product Specs
ItemFluo-8 Calcium Flux Assay Kit - No Wash
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab112129
Quantity96 test
MethodFuncS
Sample TypeAdherent cells, Suspension cells
Assay Time1h 0m
ApplicationsCellular Assay
TypeCell-based
Detection TargetCalcium

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(1) MELAS-Derived Neurons Functionally Improve by Mitochondrial Transfer from Highly Purified Mesenchymal Stem Cells (REC)Int J Mol SciDecember 6, 2023Lu Liu, Jiahao Yang, Yoshinori Otani, Takahiro Shiga, Akihiro Yamaguchi, Yasuaki Oda, Miho Hattori, Tsukimi Goto, Shuichi Ishibashi, Yuki Kawashima-Sonoyama, et al.Calcium Intracellular calcium levels were measured in each group using the Fluo-8 Calcium Flux Assay Kit (Abcam, ab112129). In brief, cells were incubated with Fluo-8 for 30 min at 37 °C and 30 min at RT in HHBS buffer (Sigma-Aldrich,

(2) NAD+ regulates nucleotide metabolism and genomic DNA replicationNature Cell BiologyNovember 13, 2023Sebastian Howen Nesgaard Munk, Joanna Maria Merchut-Maya, Alba Adelantado Rubio, Arnaldur Hall, George Pappas, Giacomo Milletti, MyungHee Lee, Lea Giørtz Johnsen, Per Guldberg, Jiri Bartek, et al.the treatment time with Fluo-8 dye-loading solution from the Fluo-8 Calcium Flux Assay Kit (Abcam, ab112129) to assess intracellular calcium levels. Fluorescence intensity was then measured on a SpectraMax iD3

(3) The potent analgesia of intrathecal 2R, 6R-HNK via TRPA1 inhibition in LF-PENS-induced chronic primary pain modelThe Journal of Headache and PainOctober 19, 2023An-Ran Liu, Zhen-Jia Lin, Ming Wei, Yuan Tang, Hui Zhang, Xiang-Ge Peng, Ying Li, Yu-Fan Zheng, Zhi Tan, Li-Jun Zhou, et al.previous research [36]. Calcium imaging DRG cells were incubated with Fluo-8 Calcium Flux Assay Kit (ab112129, Abcam) in 37 °C CO2 incubator and RT for 30 min without light exposure, and then fluorescence dye was

(4) SnRNA-seq reveals the heterogeneity of spinal ventral horn and mechanism of motor neuron axon regenerationiScienceAugust 18, 2023Zhu Y, Luan C, Gong L, Gu Y, Wang X, Sun H, Chen Z, Zhou Q, Liu C, Shan Q, et al.Calcium imaging Gabapentin (100 μM) pretreated primary motor neurons for 36h, and calcium ion indicator (Abcam, Cat# ab112129) would show the changes of Ca2+ in neurons after axon injury caused by LPA (Sigma-Aldrich, Cat# L7260)

(5) Critical role of mitogen-inducible gene 6 in restraining endothelial cell permeability to maintain vascular homeostasisJournal of cell communication and signalingMarch 1, 2023Xing L, Huang G, Chen R, Huang L, Liu J, Ren X, Wang S, Kuang H, Kumar A, Kim JK, et al.40,000 cells/100 μl/well in a 96-well plate for overnight. An equal volume (100 μl/well) of Fluo-8 dye (ab112129, Abcam) in serum-free EC media was added. After incubation for 1 h, HHBS containing VEGFA (20 ng/ml)

(6) Protocol for high-throughput single-cell patterning using a reusable ultrathin metal microstencilSTAR ProtocolsMarch 1, 2023Qingqing Tian, Kunming Xing, Yongshu Liu, Qian Wang, Haonan Sun, Ying-Nan Sun, Shusheng Zhang(FBS),BBI,Cat#04-001 Penicillin/streptomycin (P/S),Gibco,Cat#15140-122 Fluo-8 No Wash Calcium Assay Kit,Abcam,Cat#ab112129 Fibronectin,Solarbio,Cat# F8180 Tetramethylrhodamine methyl ester (TMRM),Invitrogen,Cat#T668 Staurosporine

(7) Porcine reproductive and respiratory syndrome virus infection triggers autophagy via ER stress-induced calcium signaling to facilitate virus replicationPLoS pathogensMarch 1, 2023Diao F, Jiang C, Sun Y, Gao Y, Bai J, Nauwynck H, Wang X, Yang Y, Jiang P, Liu X.measured as previously described with minor modifications [27]. A Fluo-8 Calcium Flux Analysis Kit (Abcam, ab112129) was used to measure cytoplasmic Ca2+. Briefly, at indicated timepoints, 100 μL of Fluo-8 dye-loading

(8) IP3R-dependent mitochondrial dysfunction mediates C5b-9-induced ferroptosis in trichloroethylene-caused immune kidney injuryFrontiers in immunologyJanuary 1, 2023Liu Z, Ma J, Zuo X, Zhang X, Xie H, Wang F, Wu C, Zhang J, Zhu Q.Cytosolic Ca2+ detection Cytosolic Ca2+ was detected using a Fluo-8 calcium flux assay kit (Abcam, Cat. ab112129). HK-2 cells and mouse primary tubular epithelial cells were cultured with 100 μl Fluo-8 dye-loading

(9) A metabolic associated fatty liver disease risk variant in MBOAT7 regulates toll like receptor induced outcomesNature CommunicationsDecember 6, 2022Jawaher Alharthi, Ali Bayoumi, Khaled Thabet, Ziyan Pan, Brian S. Gloss, Olivier Latchoumanin, Mischa Lundberg, Natalie A. Twine, Duncan McLeod, Shafi Alenizi, et al.measurement Intracellular Ca2+ was measured in MDMs using the Fluo-8 Calcium Flux Assay Kit (Abcam, ab112129). In brief, cells were incubated with Fluo-8 for 0.5 h at 37 °C and 0.5 hour at RT in calcium-free Hanks’

(10) Natural statin derivatives as potential therapy to reduce intestinal fluid loss in choleraPLoS neglected tropical diseasesDecember 1, 2022Noitem R, Pongkorpsakol P, Changsen C, Sukpondma Y, Tansakul C, Rukachaisirikul V, Muanprasat C.calcium measurement Intracellular Ca2+ levels were measured using the Fluo-8 Calcium Flux Assay Kit (Cat#ab112129, Abcam plc, CB2, 0AX, UK). T84 cells were seeded (1 x 105 cells/well) and cultured for 24 h on 96-black

(11) DMGV Is a Rheostat of T Cell Survival and a Potential Therapeutic for Inflammatory Diseases and CancersFrontiers in ImmunologyAugust 5, 2022Fengyuan Mandy Yang, Liya Shen, Dengxia Denise Fan, Kuan-Hung Chen, Jongdae Leeinstructions. Calcium Flux Assay Intracellular calcium was measured using the non-wash calcium assay Fluo8 kit (ab112129, Abcam) according to the manufacturer’s instructions. Briefly, T cells were spun down (500rcf, 5min),

(12) Integrin α3β1 promotes vessel formation of glioblastoma-associated endothelial cells through calcium-mediated macropinocytosis and lysosomal exocytosisNature communicationsJuly 25, 2022Bae E, Huang P, Müller-Greven G, Hambardzumyan D, Sloan AE, Nowacki AS, Marko N, Carlin CR, Gladson CL.wheat germ agglutinin-Alexa-Fluor-647-conjugate (W32466) from Thermo Fisher Scientific, and Fluo-8 dye-loading solution (ab112129) from Abcam. qRT-PCR (quantitative reverse transcription polymerase chain reaction) NECs (422 and

(13) Induction of autophagy-dependent ferroptosis to eliminate drug-tolerant human retinoblastoma cellsCell death & diseaseJune 2, 2022Liu K, Huang J, Liu J, Klionsky DJ, Kang R, Tang D.groups was calculated and normalized to protein concentration. Calcium flux assay Fluo-8 (Abcam, ab112129) is a novel green calcium indicator to monitor calcium concentration and flux in cells. Briefly, the

(14) Perivascular Fibrosis Is Mediated by a KLF10-IL-9 Signaling Axis in CD4+ T CellsCirculation researchMay 27, 2022Zhuang R, Chen J, Cheng HS, Assa C, Jamaiyar A, Pandey AK, Pérez-Cremades D, Zhang B, Tzani A, Khyrul Wara A, et al.Thermo Scientific",https://www.thermofisher.cn/order/catalog/product/88847 Fluo-8 No Wash Calcium Assay kit,"ab112129, Abcam,",https://www.abcam.com/fluo-8-calcium-fluxassay-kit-no-wash-ab112129.html Major Resources

(15) Activation of UCP1-Independent Ca2+ Cycling Thermogenesis by Wireless OptogeneticsMethods in Molecular BiologyMay 2, 2022Kenji Ikeda, Kazuki Tajima, Yuji Tanabe, Ada S. Y. Poon, Shingo KajimuraRosiglitazone (according to the previous study [12]). Fluo-8 No Wash Calcium Assay Kit (Abcam, ab112129). Hanks’ balanced salt solution (HHBS). CaCl2. L-type Ca2+ channel blocker (isradipine 10 μM).

(16) Novel role of pre-replication complex component cell division cycle 6 in retinal neovascularizationArteriosclerosis, Thrombosis, and Vascular BiologyApril 1, 2022Raj Kumar, Gadiparthi N. RaoAlto, CA). Anti-Ki67 (ab15580), and anti-pSer/Thr (ab17464) antibodies, Fluo-8 calcium flux assay kit (ab112129) and Fluo-8 AM green fluorescent calcium binding dye (ab142773) were obtained from Abcam (Cambridge,

(17) NPFF Decreases Activity of Human Arcuate NPY Neurons: A Study in Embryonic-Stem-Cell-Derived ModelInternational journal of molecular sciencesMarch 17, 2022Torz L, Niss K, Lundh S, Rekling JC, Quintana CD, Frazier SED, Mercer AJ, Cornea A, Bertelsen CV, Gerstenberg MK, et al.measured using Ca2+ indicator Fluo-8. Cells were loaded with 1 µM Fluo-8 (Calcium Flux Assay Kit, #ab112129, Abcam, Cambridge, UK) for 30 min according to manufacturer’s protocol. Recording of spontaneous calcium

(18) GPR15-C10ORF99 functional pairing initiates colonic Treg homing in amniotesEMBO reportsFebruary 3, 2022Song J, Zheng H, Xue J, Liu J, Sun Q, Yang W, Liu F, Xiang X, He K, Chen Y, et al.experiments. Intracellular calcium assay Intracellular calcium was measured using the non‐wash calcium assay Fluo‐4 kit (ab112129, Abcam) according to the manufacturer’s instructions. Briefly, CHO cells were seeded in 24‐well plates

(19) High‐dose vitamin D metabolite delivery inhibits breast cancer metastasisBioengineering & Translational MedicineJanuary 1, 2022Jiaye Liu, Junyi Shen, Chunyang Mu, Yang Liu, Dongsheng He, Han Luo, Wenshuang Wu, Xun Zheng, Yi Liu, Sunrui Chen, et al.then separated via centrifugation for calcium measurement. A fluorescence‐based Calcium Assay Kit (ab112129; Abcam) was used, according to the product instruction, to read calcium levels in serum. 52 4.14 Cal

(20) Time- and dose-dependent inhibition of neutrophil extracellular trap formation by blocking of the interleukin-1 receptorCentral European Journal of ImmunologyDecember 12, 2021Hannah Wadehn, Laia Pagerols Pagerols Raluy, Jan Kolman, Charlotte Duecker, Magdalena Trochimiuk, Birgit Appl, Michael Boettcher, Konrad Reinshagen, Julian Trahmobilization, the Fluo-8 No Wash Calcium Assay kit was used, following the manufacturer’s protocol (ab112129, Abcam). Briefly, 1 × 105 cells per well were seeded in black flat-bottom 96-well plates (Corning Incorporated,

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Fluo-8 Calcium Flux Assay Kit - No Wash from Abcam

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Fluo-8 Calcium Flux Assay Kit - No Wash from Abcam

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