Rat Leptin ELISA Kit ab100773 from Abcam

Product Specs
ItemRat Leptin ELISA Kit
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab100773
MethodELISA
Quantity1 96TESTS
ReactivityRat
ApplicationsELISA
Gene NameLEP
TypeSandwich (quantitative)
Detection Range10.97 - 8000 pg/ml
Sensitivity30 pg/ml
NCBI Gene AliasesLEPD, OB, OBS
Detection TargetLeptin

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) The Hepatic Antisteatosis Effect of Xanthohumol in High-Fat Diet-Fed Rats Entails Activation of AMPK as a Possible Protective MechanismFoodsNovember 22, 2023Hebatallah Husseini Atteia, Nora A. AlFaris, Ghedeir M. Alshammari, Eman Alamri, Salwa Fares Ahmed, Renad Albalwi, Sahar Abdel-Latif Abdel-Sattarthe Serum Samples Serum leptin levels were measured using a rat’s specific leptin ELISA kit (Cat # ab100773, Abcam, Cambridge, UK). Serum levels of aspartate aminotransferase (AST) were measured using an AST

(2) Esculeogenin A, a Glycan from Tomato, Alleviates Nonalcoholic Fatty Liver Disease in Rats through Hypolipidemic, Antioxidant, and Anti-Inflammatory EffectsNutrientsNovember 11, 2023Jwharah M. Al Jadani, Nawal A. Albadr, Ghedeir M. Alshammari, Soheir A. Almasri, Farah Fayez Alfayez, Mohammed Abdo Yahyaand Inflammation Serum levels of leptin were measured using a special rat leptin ELISA kit (Cat. # ab100773, Abcam, Cambridge, UK). Serum adiponectin levels in all collected samples were analyzed using a rat adiponectin ELISA kit

(3) Carob Extract (Ceratonia siliqua L.): Effects on Dyslipidemia and Obesity in a High-Fat Diet-Fed Rat ModelPharmaceuticsNovember 10, 2023Aleksandar Rašković, Nikola Martić, Ana Tomas, Bojana Andrejić-Višnjić, Milana Bosanac, Marko Atanasković, Marko Nemet, Radmila Popović, Marko Krstić, Saša Vukmirović, et al.cholic acid were purchased from Acros Organics, Geel, Belgium. Rat adiponectin (ab239421) and leptin (ab100773) ELISA kits were purchased from Abcam. The antibodies used for immunohistochemical staining were CYP2E1

(4) Neonatal hyperoxia leads to white adipose tissue remodeling and susceptibility to hypercaloric dietPhysiological ReportsJuly 11, 2023Alyson Deprez, Marie‐Amélie Lukaszewski, Coraline De Sousa Do Outeiro, Jéssica H. Poletto Bonetto, Ying He, Anik Cloutier, Daniela Ravizzoni Dartora, Anne Monique NuytSainte‐Justine. Leptin was measured using an enzyme‐linked immunosorbent assay (ELISA) kit (Abcam, ab100773), according to the manufacturer's instructions. 2.7 Histology and immunofluorescence Adipocyte size

(5) Activation of AMPK Entails the Protective Effect of Royal Jelly against High-Fat-Diet-Induced Hyperglycemia, Hyperlipidemia, and Non-Alcoholic Fatty Liver Disease in RatsNutrientsMarch 18, 2023Alaa Hasanain Felemban, Ghedeir M. Alshammari, Abu ElGasim Ahmed Yagoub, Laila Naif Al-Harbi, Maha H. Alhussain, Mohammed Abdo Yahyarespectively). The serum levels of leptin and adiponectin were measured using rat-specific ELISA kits (Cat. No. ab100773 and Cat. No. ab239421, Abcam, Cambridge, UK). The hepatic levels of total glutathione (GSH), malondialdehyde

(6) The Synergistic Protective Effect of γ-Oryzanol (OZ) and N-Acetylcysteine (NAC) against Experimentally Induced NAFLD in Rats Entails Hypoglycemic, Antioxidant, and PPARα Stimulatory EffectsNutrientsDecember 26, 2022Alwadani AH, Almasri SA, Aloud AA, Albadr NA, Alshammari GM, Yahya MA.supernatant of each sample was frozen at −80 °C and then used later to measure levels of leptin (Cat. No. ab100773, Abcam, Cambridge, UK), total glutathione (GSH), (Cat. No. MBS265966, MyBiosources, San Diego, CA, USA),

(7) Short-term exposure to an obesogenic diet during adolescence elicits anxiety-related behavior and neuroinflammation: modulatory effects of exogenous neuregulin-1Translational PsychiatryFebruary 26, 2022Julio David Vega-Torres, Perla Ontiveros-Angel, Esmeralda Terrones, Erwin C. Stuffle, Sara Solak, Emma Tyner, Marie Oropeza, Ike dela PeÃ±a, Andre Obenaus, Byron D. Ford, et al.measurements were made using an enzyme-linked immunosorbent assay ELISA kit (Leptin Rat ELISA Kit Abcam, ab100773; Cambridge, MA, USA) according to manufacturer’s instructions. Plates were read at 450 nm using the

(8) Supplementation with Lactiplantibacillus plantarum IMC 510 Modifies Microbiota Composition and Prevents Body Weight Gain Induced by Cafeteria Diet in RatsInternational Journal of Molecular SciencesOctober 16, 2021Maria Vittoria Micioni Di Bonaventura et al.concentration was evaluated by colorimetric method using a specific kit (Rat Leptin ELISA Kit, Abcam ab100773, Cambridge CB2 0AX, UK) following the protocol of the datasheet. 4.4. Liver and Adipose Tissue: Morphological

(9) Dietary Intervention, When Not Associated With Exercise, Upregulates Irisin/FNDC5 While Reducing Visceral Adiposity Markers in Obese RatsFrontiers in PhysiologyAugust 13, 2021Vanessa de Oliveira Furino et al.anti-inflammatory markers, an ELISA analysis was performed. The cytokines IL-1β (ab255730) and leptin (ab100773) were purchased by Abcam® (Cambridge, United Kingdom and the cytokine IL-10 (no.555134) was purchased

(10) Interactive effects of compounding multidimensional stressors on maternal and male and female rat offspring outcomesHormones and BehaviorAugust 1, 2021Arielle R. Strzelewicz, Haley A. Vecchiarelli, Alejandro N. Rondón-Ortiz, Anthony Raneri, Matthew N. Hill, Amanda C. Kentnerfrozen on dry ice. Tissues were stored in −70 °C for future processing. 2.8. ELISA Plasma leptin (ab100773, Abcam) and corticosterone (ADI-900–097, Enzo Life Sciences, Farmingdale, NY, USA) levels were analyzed

(11) Roux-en-Y gastric bypass contributes to weight loss-independent improvement in hypothalamic inflammation and leptin sensitivity through gut-microglia-neuron-crosstalkMolecular metabolismJune 1, 2021Chen J, Haase N, Haange SB, Sucher R, Münzker J, Jäger E, Schischke K, Seyfried F, von Bergen M, Hankir MK, et al.fluorescent microscope (Zeiss ApoTome, Germany). 2.10 ELISA Plasma leptin levels were measured by an ELISA kit (Ab100773, Abcam, USA) according to the manufacturer's protocol. Circulating cytokine levels were measured using

(12) Leptin Receptors Are Not Required for Roux-en-Y Gastric Bypass Surgery to Normalize Energy and Glucose Homeostasis in RatsNutrientsMay 4, 2021Mohammed K. Hankir, Laura Rotzinger, Arno Nordbeck, Caroline Corteville, Ulrich Dischinger, Juna-Lisa Knop, Annett Hoffmann, Christoph Otto, Florian Seyfried(EMD Millipore, MA, USA, #EZGLP1T-36 K), plasma leptin using a Rat Leptin ELISA kit (Abcam, Cambridge, UK, #ab100773), plasma alanine transaminase (ALT) using a Rat ALT Simplestep® ELISA kit (Abcam, Cambridge, UK #ab264579),

(13) Effect of Caesalpinia bonduc Polyphenol Extract on Alloxan-Induced Diabetic Rats in Attenuating Hyperglycemia by Upregulating Insulin Secretion and Inhibiting JNK Signaling PathwayOxidative Medicine and Cellular LongevityMarch 17, 2020Asra Iftikhar, Bilal Aslam, Maryam Iftikhar, Wafa Majeed, Mehwish Batool, Bushra Zahoor, Naseem Amna, Hareem Gohar, Iqra LatifBioSure, California, USA). Serum leptin and peptide YY (PYY) levels were also assayed through ELISA kits (ab100773, Abcam, Cambridge, UK & EIAR-PYY, RayBio®, Norcross, GA, USA). The serum amylin level was determined

(14) Lupine as a potential agent against diet-induced obesity through adenosine monophosphate-activated protein kinase pathway: crude oil versus nanoemulsion formulationsBrazilian Journal of Pharmaceutical SciencesJanuary 1, 2020Omar Mahmoud Mohafez, Ibrahim Abdelrahman Alhaider, Tamer Mohamed Shehata, Maged Elsayed Mohamedfrom Sigma-Aldrich Co. (St Louis, MO, USA). Leptin rat enzyme-linked immunosorbent assay (ELISA) kit (ab100773) and adiponectin rat ELISA kit (ab108784) were obtained from Abcam plc (Cambridge, UK). A rat insulin ELISA

(15) Ginger Extract Ameliorates Renal Damage in High Fat Diet-Induced Obesity in Rats: Biochemical and Ultrastructural StudyInternational Journal of MorphologyJune 1, 2019Mashael Mohammed Bin-Meferij, Attalla Farag El-Kott, Ali Abdullah Shati, Refaat A EidUniversity, Riyadh, Saudi Arabia. . Biochemical assays: Serum leptin kit was acquired from Abcam (USA; Cat. No. ab100773). Total antioxidants (TAS) in serum was performed using assay kit from Abnova (Taiwan; Cat. No. KA0802).

(16) Access to a high resource environment protects against accelerated maturation following early life stress: A translational animal model of high, medium and low security settingsHormones and BehaviorMay 1, 2019Arielle R. Strzelewicz, Ordoñes Sanchez Ordoñes Sanchez, Alejandro N. Rondón-Ortiz, Anthony Anthony, Sydney T. Famularo, Debra A. Bangasser, Amanda C. Kentnercoefficients of variation were 6.6% and 7.8%, respectively. The minimum detectable leptin concentration (abcam ab100773, Abcam) was 30 pg/mL, intra and inter-assay coefficients of variation were <10% and <12%, respectively. 2.10 PCR Author

(17) Smallanthus sonchifolius (Yacon) Flour Improves Visceral Adiposity and Metabolic Parameters in High-Fat-Diet-Fed RatsJournal of ObesityOctober 28, 2018Stella Maris Honoré, Maria Virginia Grande, Jorge Gomez Rojas, Sara Serafina Sánchezinterleukin-1β (IL-1β) (RAB0277), insulin (RAB0904) (all from SIGMA Aldrich, St. Louis, MO, USA), leptin (ab100773), and adiponectin (ab108784) (Abcam, USA) were performed using commercially available kits according

(18) Leptin Mediates a Glucose-Fatty Acid Cycle to Maintain Glucose Homeostasis in StarvationCellJanuary 11, 2018Perry RJ, Wang Y, Cline GW, Rabin-Court A, Song JD, Dufour S, Zhang XM, Petersen KF, Shulman GI.Assays,Critical Commercial Assays Rat insulin ELISA,Mercodia,CAT#10-1250 Rat leptin ELISA,Abcam,CAT#ab100773 Rat CRH ELISA,MyBioSource,CAT#MBS703710 Rat ACTH ELISA,MyBioSource,CAT#MBS2502683 Mouse/rat corticosterone ELISA

(19) Leptin Signaling in the Carotid Body Regulates a Hypoxic Ventilatory Response Through Altering TASK Channel ExpressionFrontiers in physiologyJanuary 1, 2018Yuan F, Wang H, Feng J, Wei Z, Yu H, Zhang X, Zhang Y, Wang S.kept on ice. After centrifugation, the plasma was stored at−80°C for leptin analysis by ELISA kit (#ab100773, Abcam, USA), an in vitro enzyme-linked immunosorbent assay for the quantitative measurement as previously

(20) Long-Term Sex-Dependent Vulnerability to Metabolic challenges in Prenatally Stressed RatsFrontiers in Behavioral NeuroscienceJune 29, 2017Pamela Panetta, Alessandra Berry, Veronica Bellisario, Sara Capoccia, Carla Raggi, Alessia Luoni, Linda Longo, Marco A. Riva, Francesca Cirullilevels of plasma adipokines (leptin and adiponectin) were assessed using Rat Leptin Elisa kit (Abcam®–ab100773, Cambridge, MA) and Rat Adiponectin Elisa kit (Abcam®–ab108784, Cambridge, MA) respectively. Measurements

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Rat Leptin ELISA Kit from Abcam

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Rat Leptin ELISA Kit from Abcam

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