Cdc42 G-LISA Activation Assay Kit BK127 from Cytoskeleton, Inc.

Product Specs
ItemCdc42 G-LISA Activation Assay Kit
CompanyCytoskeleton, Inc.
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberBK127
Detection TargetCdc42
QuantityKIT 96 assays
MethodCdc42 G-LISA Assay Kits
ApplicationsELISA
Brand/TrademarkCytoskeleton, Inc

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Cytoskeleton, Inc.

1830 S. Acoma St.

Denver, Colorado 80223

United States

Phone: 303 322-2254
303 322-2257

Company Profile

Website: http://cytoskeleton.com/

(1) The Rac inhibitor HV-107 as a potential therapeutic for metastatic breast cancerMolecular MedicineJune 14, 2023Grace Velez Crespo, Jescelica Ortiz, Eliud Hernández O’Farrill, Cornelis P. Vlaar, Mikhail Inyushin, Yuriy Kucheryavykh, Lilia Kucheryavykh(0.1% DMSO), 100, 250, 500, 1000, and 2000 nM HV-107 for 24 h. Lysates were subjected to active Cdc42 (#BK127, Cytoskeleton, Denver, CO, USA) or active RhoA (#BK124, Cytoskeleton, Denver, CO, USA) Glisa colorimetric

(2) FXR1 regulates vascular smooth muscle cell cytoskeleton, VSMC contractility, and blood pressure by multiple mechanismsCell reportsApril 25, 2023St Paul A, Corbett C, Peluzzo A, Kelemen S, Okune R, Haines DS, Preston K, Eguchi S, Autieri MV.Polymerization Kit,"Cytoskeleton, Inc",Cat# BK037 CDC42 G-LISA Activation Kit,"Cytoskeleton, Inc",Cat #BK127 Rac1 G-LISA Activation Kit,"Cytoskeleton, Inc",Cat #BK128 ,, Deposited data,Deposited data,Deposited

(3) PKCε inhibition prevents ischemia‑induced dendritic spine impairment in cultured primary neuronsExperimental and Therapeutic MedicineFebruary 16, 2023Chenjie Ge, Xuefeng Wang, Yunhong Wang, Lilei Lei, Guohua Song, Mincai Qian, Shiliang Wangdifferent G-LISA activation assay kits (cat. nos. BK124 for active RhoA, BK128 for active Rac1 and BK127 for CDC42; Cytoskeleton, Inc.) following the manufacturer's instructions. Briefly, high-density cortical

(4) CDC42 governs normal oviduct multiciliogenesis through activating AKT to ensure timely embryo transportCell death & diseaseSeptember 2, 2022Jiang R, Tang X, Pan J, Li G, Yang N, Tang Y, Bi S, Cai H, Chen Q, Chen D, et al.CDC42 or RAC1 were detected by western blot. G-LISA Cdc42 Activation Assay Biochem Kit (Cytoskeleton, BK127) was used to measure levels of activated CDC42 in organoids treated with CDC42 activity inhibitors was

(5) The Hippo pathway mediates Semaphorin signalingScience AdvancesMay 27, 2022Zhipeng Meng et al.RhoA/Rac1 interacting with Rho-GTP–binding strips using G-LISA kits (Cytoskeleton Inc. #BK124 and #BK127). Briefly, 25 μg of cell lysates of each group was incubated with Rho-GTP–binding strips at 4°C for

(6) Glucocorticoid-mediated induction of caveolin-1 disrupts cytoskeletal organization, inhibits cell migration and re-epithelialization of non-healing woundsCommunications BiologyDecember 1, 2021Ivan Jozic et al.assays were performed according to manufacturer’s instructions (Cytoskeleton Inc., RhoA: #BK124; Cdc42: #BK127). Briefly, cells were grown to 60% confluency and serum starved overnight prior to assessment of Cdc42/RhoA

(7) Non-canonical glutamine transamination sustains efferocytosis by coupling redox buffering to oxidative phosphorylationNature MetabolismOctober 1, 2021Johanna Merlin et al.Rac1 G-LISA Activation Assay Kit (Cytoskeleton BK128) and Cdc42 G-LISA Activation Assay Kit (Cytoskeleton BK127) was used according to the manufacturer’s instructions. G-Actin/F-Actin assay G-Actin/F-Actin activity

(8) miR‑769‑5p is associated with prostate cancer recurrence and modulates proliferation and apoptosis of cancer cellsExperimental and Therapeutic MedicineFebruary 8, 2021Daniel Leeon ice as per the instructions for the CDC42 G-LISA Activation Assay (Cytoskeleton, Inc.; cat. no. BK127). Protein concentrations were measured according to the manufacturer's instructions using Precision

(9) Ror2-mediated non-canonical Wnt signaling regulates Cdc42 and cell proliferation during tooth root developmentDevelopment (Cambridge, England)January 21, 2021Ma Y, Jing J, Feng J, Yuan Y, Wen Q, Han X, He J, Chen S, Ho TV, Chai Y.protein in tissue lysates was detected using Cdc42 or Rac1 G-LISA assay, respectively (Cytoskeleton, BK127/BK128). The level of activation was measured by reading at OD490 nm. TUNEL assays Apoptotic cells

(10) Reelin Amplifies Glycoprotein VI Activation and AlphaIIb Beta3 Integrin Outside-In Signaling via PLC Gamma 2 and Rho GTPasesArteriosclerosis, Thrombosis, and Vascular BiologyOctober 1, 2020Irena Krueger, Lothar Gremer, Lena Mangels, Meike Klier, Kerstin Jurk, Dieter Willbold, Hans H. Bock, Margitta ElversRhoA, and CDC42 Activation Assay Biochem Kits (Absorbance Based; Cytoskeleton, Inc, BK128, BK124, and BK127) were used according to the manufacturer’s protocol. Histology After FeCl3-induced injury, the occluded

(11) mTOR signaling regulates the morphology and migration of outer radial glia in developing human cortexeLifeSeptember 2, 2020Andrews MG, Subramanian L, Kriegstein AR.through the use of an ELISA-based Rho-GTPase activation assay kit (Cytoskeleton, Inc, catalog# BK124, BK127, BK128). Briefly, protein was isolated from the SVZ of microdissected slice cultures after treatment

(12) Elevating EGFR-MAPK program by a nonconventional Cdc42 enhances intestinal epithelial survival and regenerationJCI insightAugust 20, 2020Zhang X, Bandyopadhyay S, Araujo LP, Tong K, Flores J, Laubitz D, Zhao Y, Yap G, Wang J, Zou Q, et al.respectively. CDC42 activity was determined before and after treatment by CDC42-specific G-LISA assay (BK127, Cytoskeleton) performed according to the manufacturer’s instructions. Lentivirus-mediated Cdc42 knockdown. To

(13) Compressive Stimulation Enhances Ovarian Cancer Proliferation, Invasion, Chemoresistance, and Mechanotransduction via CDC42 in a 3D BioreactorCancersJune 10, 2020Novak CM, Horst EN, Lin E, Mehta G.Activation and Inhibition) To assess CDC42 activation, a G-Lisa assay from Cytoskeleton Inc. (Cat. # BK127) was used. Sample preparation procedures were followed as directed in kit instructions with few modifications.

(14) Knockout of ACTB and ACTG1 with CRISPR/Cas9(D10A) Technique Shows that Non-Muscle β and γ Actin Are Not Equal in Relation to Human Melanoma Cells' Motility and Focal Adhesion FormationInternational journal of molecular sciencesApril 15, 2020Malek N, Mrówczyńska E, Michrowska A, Mazurkiewicz E, Pavlyk I, Mazur AJ.Denver, CO, USA) were used to compare the levels of activated Rac1 (BK128), RhoA (BK124), and Cdc42 (BK127) GTPases in different groups of cells after LPA and PMA stimulations. Lysates were prepared according

(15) RHOA-mediated mechanical force generation through Dectin-1Journal of Cell ScienceMarch 1, 2020Rohan P. Choraghe, Tomasz Kołodziej, Alan Buser, Zenon Rajfur, Aaron K. Neumannstimulation. G-LISA The G-LISA activation assay kit RHOA (Cat. #BK124), RAC1 (Cat. #BK128) and CDC42 (Cat. #BK127) from Cytoskeleton, Inc. was used according to manufacturer instructions to measure active GTPase. Dectin-1-transfected

(16) The p75NTR Influences Cerebellar Circuit Development and Adult Behavior via Regulation of Cell Cycle Duration of Granule Cell ProgenitorsThe Journal of neuroscience : the official journal of the Society for NeuroscienceNovember 13, 2019Zanin JP, Verpeut JL, Li Y, Shiflett MW, Wang SS, Santhakumar V, Friedman WJ.according to the manufacturer’s instructions. G-Lisa for RhoA (Cytoskeleton, #BK124), Cdc42 (Cytoskeleton, #BK127), and Rac1 (Cytoskeleton, #BK128). RhoA inhibition experiments. Cells were obtained from P7 pups and cultured

(17) Wnt5a induces ROR1 and ROR2 to activate RhoA in esophageal squamous cell carcinoma cellsCancer management and researchJanuary 1, 2019Wu X, Yan T, Hao L, Zhu Y.assays (G-LISA small GTPase activation assays) Rho GTPase activation assays (Cat. BK121 for RhoA, Cat. BK127 for Cdc42, Cat. BK125 for Rac1,2,3, Cytoskeleton Inc., Denver, CO, USA) were used to measure the Rho

(18) Nbeal2 interacts with Dock7, Sec16a, and Vac14BloodMarch 1, 2018Louisa Mayer et al.UK) were done at room temperature for different time points, and lysis buffer provided by assay kit (BK127 and BK128, Cytoskeleton) was added. Subsequent steps were carried out following the manufacturer’s protocol.

(19) Inhibition of farnesyl pyrophosphate synthase improves pressure overload induced chronic cardiac remodelingScientific ReportsDecember 23, 2016Chen-Ze Zhao, Xu-Ming Zhao, Jian Yang, Yun Mou, Bin Chen, Huan-Dong Wu, Dong-Pu Dai, Jie Ding, Shen-Jiang Hudetermined by an absorbance-based G-LISA Activation Assay Biochemistry kit (Kit #BK131; #BK124; #BK128; #BK127; Cytoskeleton, Denver, CO, USA). Ras activity was determined from protein isolated from left ventricular

(20) Extracellular translationally controlled tumor protein promotes colorectal cancer invasion and metastasis through Cdc42/JNK/ MMP9 signalingOncotargetAugust 2, 2016Bin Xiao et al.Cdc42 and Rac1) activation was measured using the Small G-proteins Activation Kits (Cat. BK124, Cat. BK127 and Cat. BK128. Cytoskeleton, Denver, USA). according to the manufacturer's instructions. The activation

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Cdc42 G-LISA Activation Assay Kit from Cytoskeleton, Inc.

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Cdc42 G-LISA Activation Assay Kit from Cytoskeleton, Inc.

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