Fig 1: (A) Stereomicroscope images of pituitary glands of Pou1f1WT/WT and Pou1f1GE/GE mice at 12 weeks of age. Scale bar, 1 mm. (B) Pituitary gland weight per body weight of Pou1f1WT/WT, Pou1f1WT/GE, and Pou1f1GE/GE mice. Data represent the mean ± SD. *P < 0.05 vs Pou1f1WT/WT mice. (C) Histology of pituitary glands at 12 weeks of age. Upper images show hematoxylin and eosin staining. Lower images depict immunostaining with anti-GH antibody. Scale bar, 100 µm. (D, E) Serum IGF-1 and thyroxine values of 12 weeks of age, respectively. Data represent the mean ± SD. *P < .05 vs Pou1f1WT/WT mice. **P < .01; n.s., not significant.
Fig 2: IGF-1 measurements. Comparison of IGF-1 in the early (A), middle (B), and late phases (C). Means and standard errors of IGF-1 are shown as the vertical and the error bars, respectively. Statistical analysis was performed using Student's t-test (*p < 0.05)
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