Fig 1: CBL0137 did not affect the acetylation and expression of H3 histone, the activity of histone acetyltransferases, or HDAC1 and HDAC3 expression: (A) Histone H3 acetylation was assessed using Western blotting after treatment of HeLa TI cells with CBL0137 (1.2 and 0.6 μM) or TSA (0.25 μM, used as positive control) for 24 h. Western blotting results were normalized to histone H4 and are shown as mean ± SD of the densitometric analysis (n ≥ 3); * p < 0.05 compared to vehicle control (C). (B) Enzymatic activity of the HAT family proteins was assessed using Histone Acetyltransferase Activity Assay Kit after treatment of HeLa TI cells with CBL0137 (1.2 and 0.6 μM) for 24 h. The results are shown as mean ± SD (n ≥ 3); * p < 0.05 compared to vehicle control. (C) HAT1, CREBBP, and EP300 gene expressions were assessed with RT-qPCR after treatment of HeLa TI cells with CBL0137 (1.2 and 0.6 μM) for 24 h. The results were normalized to the housekeeping gene RPLP0 expression and are shown as mean ± SD (n ≥ 3). The 2−ΔΔCt method was used for data calculation. (D) Expressions of HDAC1 and HDAC3 were assessed using Western blotting after treatment of HeLa TI cells with CBL0137 (1.2 and 0.6 μM) for 24 h. The results were normalized to β-actin and are shown as mean ± SD of the densitometric analysis (n ≥ 3).