LDH Assay Kit (Cytotoxicity) ab65393 from Abcam

Product Specs
ItemLDH Assay Kit (Cytotoxicity)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab65393
MethodFuncS
Quantity500 test
Sample TypeAdherent cells, Suspension cells
Assay Time1h 0m
ApplicationsCellular Assay
Gene NameLDHC
NCBI Gene AliasesGSD11, HEL-S-133P, LDHM, PIG19
Detection TargetLactate Dehydrogenase

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Enteropathogenic E. coli infection co-elicits lysosomal exocytosis and lytic host cell deathmBioDecember 1, 2023Raisa Shtuhin-Rahav, Aaron Olender, Efrat Zlotkin-Rivkin, Etan Amse Bouman, Tsafi Danieli, Yael Nir-Keren, Aryeh M. Weiss, Ipsita Nandi, Benjamin Aroeti(600 g, 10 min), and the LDH released into them was measured by the LDH-cytotoxicity assay (Abcam #ab65393), as described in the manufacturer’s protocol. The percentage LDH release (i.e., cytotoxicity [%]) =

(2) Dexmedetomidine Ameliorates Cardiac Ischemia/Reperfusion Injury by Enhancing Autophagy Through Activation of the AMPK/SIRT3 PathwayDrug Design, Development and TherapyOctober 25, 2023Hong He, Peng Liu, Peng Liat 37°C for 24 h in humidified air containing 5% CO2 for reperfusion. LDH Release LDH assay kits (#ab65393) were purchased from Abcam Inc. (Boston, MA, USA) and used to determine the LDH release in the supernatant

(3) Neuronal RBM5 modulates cell signaling responses to traumatic and hypoxic-ischemic injury in a sex-dependent mannerCell Death DiscoveryOctober 17, 2023Kara Snyder, Kiersten Gorse, Patrick M. Kochanek, Travis C. JacksonQuickTiter Kit, Cat# VPK-107 (Cell Biolabs, Inc). Cell Injury Supplies: LDH Release Assay Kit, Cat#ab65393 (Abcam), BioFlex untreated culture plates, Cat#BF-3001U (FlexCell International). Cell Recovery Solution,

(4) Gut microbial change after administration of Lacticaseibacillus paracasei AO356 is associated with anti-obesity in a mouse modelFront Endocrinol (Lausanne)August 29, 2023Eun-Ji Song, Eun-Sook Lee, Young In Kim, Dong-Uk Shin, Ji-Eun Eom, Hee Soon Shin, So-Young Lee, Young-Do NamThe toxicity of AO356 was determined using an LDH assay kit following the manufacturer’s protocol (ab65393, Abcam, Cambridge, UK). The non-treated culture medium was used as a low control, the cell lysis buffer

(5) A placental model of SARS-CoV-2 infection reveals ACE2-dependent susceptibility and differentiation impairment in syncytiotrophoblastsNature Cell BiologyJuly 13, 2023J. Chen, J. A. Neil, J. P. Tan, R. Rudraraju, M. Mohenska, Y. B. Y. Sun, E. Walters, N. G. Bediaga, G. Sun, Y. Zhou, et al.between 1/1,000 to 1/2,000 before analysis. LDH was measured using the Abcam LDH cytotoxicity kit II (ab65393) according to the manufacturer’s instructions in undiluted supernatants using an LDH standard curve.

(6) Lats2 deficiency protects the heart against myocardial infarction by reducing inflammation and inhibiting mitochondrial fission and STING/p65 signalingInternational Journal of Biological SciencesJuly 3, 2023Libao Liu, Shuai Huang, Yingzhen Du, Hao Zhou, Kai Zhang, Jinyuan Heusing Cell Counting Kit 8 (Cat. No. ab228554, Abcam) and an LDH Assay Kit (Cytotoxicity) (Cat. No. ab65393, Abcam) in accordance with the supplier's protocols 33. Immunoblot analysis Frozen hearts and/or cells

(7) Human Oral Mucosa Stem Cells Increase Survival of Neurons Affected by In Vitro Anoxia and Improve Recovery of Mice Affected by Stroke Through Time-limited Secretion of miR-514A-3pCellular and molecular neurobiologyJuly 1, 2023Stančin P, Song MS, Alajbeg I, Mitrečić D.visualized by Live Dead (Thermofisher L3224) and the LDH assay was performed using a LDH kit (Abcam, ab65393). All quantifications were performed by analysing five visual fields in three independent experiments. Overexpression

(8) TXNIP contributes to induction of pro-inflammatory phenotype and caspase-3 activation in astrocytes during Alzheimer's diseasesRedox biologyJuly 1, 2023Kim J, Lim J, Yoo ID, Park S, Moon JS.using culture medium of human astrocytes with lactate dehydrogenase (LDH) Assay Kit (Cytotoxicity) (ab65393, Abcam) following the manufacturer’s instructions. Briefly, human astrocytes (2 × 105 cells in 6-well

(9) Topology and function of translocated EspZmBioJune 21, 2023Nir Haritan, Etan Amse Bouman, Ipsita Nandi, Raisa Shtuhin-Rahav, Efrat Zlotkin-Rivkin, Tsafi Danieli, Naomi Melamed-Book, Yael Nir-Keren, Benjamin Aroetig, 10 minutes), and the LDH released into them was measured by the LDH-Cytotoxicity Assay (Abcam no. ab65393), as described in the manufacturer’s protocol. The % LDH release was calculated as follows, Cytotoxicity(%)=Test

(10) Chitosomes Loaded with Docetaxel as a Promising Drug Delivery System to Laryngeal Cancer Cells: An In Vitro Cytotoxic StudyInternational Journal of Molecular SciencesJune 8, 2023Christian R. Moya-Garcia, Nicole Y. K. Li-Jessen, Maryam Tabrizian(cat. # C581-25) was purchased from Fisher Scientific (Ottawa, ON, Canada). The LDH assay kit (cat. # ab65393), ALEXA647/EGFR (cat. # ab192982), ALEXA488/TUBIII (cat. # ab195879), and DAPI (cat. # ab228549) from

(11) Botulinum neurotoxin A modulates the axonal release of pathological tau in hippocampal neuronsToxiconJune 1, 2023Chiara Panzi, Sunaina Surana, Samantha De La-Rocque, Edoardo Moretto, Oscar Marcelo Lazo, Giampietro Schiavosomatic side of the same MFCs used for the ELISA was analysed with the Cytotoxicity LDH assay kit (Abcam #ab65393) following manufacturer's instructions. Media samples were spun at 10,000 g for 2 min and 20 μl per

(12) Image-Guided Enhanced PDT/PTT Combination Therapy Using Brominated Hemicyanine-Loaded Folate Receptor-Targeting Ag2S Quantum DotsBioconjugate ChemistryMay 17, 2023Eda Celikbas, Ayca Saymaz, Hande Gunduz, Irem Koc, Ece Cakir, Alphan Sennaroglu, Safacan Kolemen, Havva Yagci Acar, Kubra OnbasliAssay Cell membrane integrity was examined using a lactate dehydrogenase (LDH) Assay Kit (Abcam, Cat. ab65393) following the manufacturer’s protocol. HeLa cells were treated with AS-GSH, AS-GSH-FA, AS-GSH-FA/Hemi-Br,

(13) Differentiated Neurons Are More Vulnerable to Organophosphate and Carbamate Neurotoxicity than Undifferentiated Neurons Due to the Induction of Redox Stress and Accumulate Oxidatively-Damaged ProteinsBrain SciencesApril 26, 2023Anusha W. Mudyanselage, Buddhika C. Wijamunige, Artur Kocon, Wayne G. Carterof active extracellular LDH in response to pesticide exposures was measured using an LDH assay kit (ab65393, Abcam, Cambridge, UK) according to the manufacturer’s instructions. After agent treatment (1–200 µM,

(14) Engineering BinB Pore-Forming Toxin for Selective Killing of Breast Cancer CellsToxins (Basel)April 18, 2023Tipaporn Kumkoon, Chalongrat Noree, Panadda Boonsermdehydrogenase (LDH, Tokyo, Japan) leakage was determined using LDH-Cytotoxicity Assay Kit II (Abcam, ab65393, Cambridge, MA, USA) as described in the manufacturer’s protocol. The absorbance was measured at 450

(15) The IgV domain of the poliovirus receptor alone is immunosuppressive and binds to its receptors with comparable affinityScientific ReportsMarch 21, 2023Shrayasee Saha, Amanda Sparkes, Esther I. Matus, Peter Lee, Jean Gariépy37°C, supernatants were collected, and cell cytotoxicity assayed using a lactate dehydrogenase kit (ab65393; Abcam). Percent cytotoxicity was calculated with the following formula: cytotoxicity (%) = ((Test Sample

(16) Discrepant Activation Pattern of Inflammation and Pyroptosis Induced in Dermal Fibroblasts in Response to Dengue Virus Serotypes 1 and 2 and Nonstructural Protein 1Microbiology spectrumFebruary 14, 2023Wei KC, Wei WJ, Liao CL, Chang TH.repeated at least three times. LDH cytotoxicity assay. The LDH cytotoxicity assay (catalog number ab65393, Abcam, Cambridge, MA, USA) was used to detect LDH released from damaged cells. WS1 cells (1 × 104/well)

(17) HIF2α activation and mitochondrial deficit due to iron chelation cause retinal atrophyEMBO molecular medicineFebruary 8, 2023Kong Y, Liu PK, Li Y, Nolan ND, Quinn PMJ, Hsu CW, Jenny LA, Zhao J, Cui X, Chang YJ, et al.with the iMark microplate reader (BIO‐RAD, #1683315). In parallel, a colorimetric LDH assay (Abcam, ab65393) was performed to determine cytotoxicity associated with DFO. The supernatant (10 μl) of each well from

(18) High glucose and high lipid induced mitochondrial dysfunction in JEG-3 cells through oxidative stressOpen Life SciencesFebruary 7, 2023Yang Duan, Fuqiang Sun, Yueqin Li, Suyan Yang450 nm. 2.5 LDH leakage and cell oxidative stress level detection Cytotoxicity assay kit (Cat. No.: ab65393) purchased from Abcam was used to measure LDH activity. A microplate reader (BioTek, USA) was utilized

(19) Staphylococcus aureus stimulates neutrophil itaconate production that suppresses the oxidative burstCell ReportsFebruary 1, 2023Kira L. Tomlinson, Sebastián A. Riquelme, Swikrity Upadhyay Baskota, Marija Drikic, Ian R. Monk, Timothy P. Stinear, Ian A. Lewis, Alice S. Prince,Fisher Scientific,AM1906 High Capacity cDNA Reverse transcription kit,Applied Biosystems,43688-14 LDH Release Assay,Abcam,ab65393 Albumin (BCG) Assay Kit (Colorimetric),Abcam,ab235628 Deposited data,Deposited data,Deposited data Raw

(20) Antitumor Effect of Korean Red Ginseng through Blockade of PD-1/PD-L1 Interaction in a Humanized PD-L1 Knock-In MC38 Cancer Mouse ModelInternational Journal of Molecular SciencesJanuary 18, 2023Eun-Ji Lee, Ju-Hye Yang, Hye Jin Yang, Chong-Kwan Cho, Jang-Gi Choi, Hwan-Suck Chung(Dojindo Molecular Technologies, Inc., Rockville, MD, USA). A lactate dehydrogenase (LDH) assay kit (#ab65393, Abcam, Cambridge, UK) was used to measure the cytotoxic activity on target cells via effector cells

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2/11/2022Sensitive and Easy Assay to Detect Cytotoxicity Helena AiresThis cytotoxicity assay enables the analysis of cytotoxicity caused by drugs or compounds without complicated and time-consuming steps.

LDH Assay Kit (Cytotoxicity) from Abcam

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LDH Assay Kit (Cytotoxicity) from Abcam

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