Protein G Magnetic Beads from New England Biolabs

Product Specs
ItemProtein G Magnetic Beads
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberS1430S
Capacity1 ml of Protein G Magnetic Beads will bind > 400 µg of Human IgG.
Matrixnonporous superparamagnetic bead
Particle Size1 µm
Quantity1 ml
ApplicationsProteomics & Glycomics
Brand/TrademarkNew England Biolabs Inc (NEB)
Product TypeResins, Magnetic Beads
ModeAntibody Purification
TypeMagnetic Beads
Temperature4 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Peptidylarginine deiminase 2 citrullinates MZB1 and promotes the secretion of IgM and IgAFrontiers in ImmunologyNovember 29, 2023Benjamin Geary, Bo Sun, Ronak R. Tilvawala, Leonard Barasa, Konstantin Tsoyi, Ivan O. Rosas, Paul R. Thompson, I-Cheng Hocentrifuged to pellet debris. The lysate was pre-cleared with Protein G Magnetic Beads (New England BioLabs #S1430S) and rabbit IgG (Cell Signaling Technology #2729S) for 1 hour at 4°C with agitation. Pre-cleared lysate

(2) The AMPK-like protein kinases Sik2 and Sik3 interact with Hipk and induce synergistic tumorigenesis in a Drosophila cancer modelFrontiers in Cell and Developmental BiologyOctober 3, 2023Kewei Yu, Niveditha Ramkumar, Kenneth Kin Lam Wong, Gritta Tettweiler, Esther M. Verheyenovernight, followed by incubation with a mixture of protein A (Invitrogen 10002D) and protein G (NEB S1430S) magnetic beads for 3 h at 4°C. The beads were washed with lysis buffer and boiled in 4× SDS sample

(3) IGF2BP3 promotes the progression of colorectal cancer and mediates cetuximab resistance by stabilizing EGFR mRNA in an m6A-dependent mannerCell Death & DiseaseSeptember 1, 2023Li-Jie Chen, Hui-Ye Liu, Zhi-Yuan Xiao, Ting Qiu, Dan Zhang, Ling-Jie Zhang, Fang-Yi Han, Guo-Jun Chen, Xue-Mei Xu, Jiong-Hua Zhu, et al.anti-m6A antibody (NEB) was applied for m6A immunoprecipitation. Anti-m6A antibody was pre-bound to Protein G magnetic beads in reaction buffer for 30 min. The fragmented mRNA was incubated with m6A-antibody-bound protein G magnetic beads

(4) Changes in m6A RNA methylation of goat lung following PPRV infectionHeliyonSeptember 1, 2023Raja Ishaq Nabi Khan, Manas Ranjan Praharaj, Waseem Akram Malla, Neelima Hosamani, Shikha Saxena, Bina Mishra, Kaushal Kishor Rajak, Muthuchelvan Dhanavelu, Ashok Kumar Tiwari, Basavaraj Sajjanar, et al.was subjected to immunoprecipitation using m6A specific antibody (NEB #E1610S). Initially, 30 μg of Protein G magnetic beads (NEB #S1430) was washed with 250 μl of Immunoprecipitation Reaction Buffer (150 mM NaCl, 10 mM Tris-HCl,

(5) Interaction of L1CAM with LC3 Is Required for L1-Dependent Neurite Outgrowth and Neuronal SurvivalInternational journal of molecular sciencesAugust 7, 2023Loers G, Kleene R, Granato V, Bork U, Schachner M.fractions. Nuclear and non-nuclear fractions were used for immunoprecipitation. For immunoprecipitation, Protein G magnetic beads (25 μL per sample) were washed twice in PBS, pH 7.4, and incubated in dilution buffer (1 mg/mL bovine

(6) The importance of m6A topology in chicken embryo mRNA: a precise mapping of m6A at the conserved chicken β-actin zipcodeRNAJune 1, 2023Francis Baron, Mi Zhang, Nathan Archer, Eleanor Bellows, Helen M. Knight, Simon Welham, Catrin S. Rutland, Nigel P. Mongan, Christopher J. Hayes, Rupert G. Fray, et al.was used for the IP, and 1 µL for preparing the input libraries. The fragmented RNA was mixed with Protein G Magnetic Beads prebound monoclonal anti-m6A antibody (1 µL) from the EpiMark N6-Methyladenosine Enrichment Kit (New England Biolabs

(7) N(6)-Methyladenosine RNA Modification in Host Cells Regulates Peste des Petits Ruminants Virus ReplicationMicrobiology spectrumFebruary 14, 2023Khan O, Tanuj GN, Choravada DR, Rajak KK, Chandra Sekar S, Lingaraju MC, Dhara SK, Gupta PK, Mishra BP, Dutt T, et al.EpiMark N6-methyladenosine enrichment kit (E1610S; NEB) following the manufacturer’s instructions. Briefly, m6A antibody was attached to protein G magnetic beads by incubation with orbital rotation for 30 min at 4°C. The prepared RNA was bound to the beads coated

(8) The Interactions of the 70 kDa Fragment of Cell Adhesion Molecule L1 with Topoisomerase 1, Peroxisome Proliferator-Activated Receptor γ and NADH Dehydrogenase (Ubiquinone) Flavoprotein 2 Are Involved in Gene Expression and Neuronal L1-Dependent FunctionsInternational journal of molecular sciencesJanuary 20, 2023Loers G, Kleene R, Bork U, Schachner M.fraction. Nuclear and non-nuclear fractions were used for immunoprecipitation. For immunoprecipitation, Protein G magnetic beads (25 μL per sample) were washed twice in phosphate-buffered saline (PBS), pH 7.4, and incubated in dilution

(9) Matricellular Protein SMOC2 Potentiates BMP9-Induced Osteogenic Differentiation in Mesenchymal Stem Cells through the Enhancement of FAK/PI3K/AKT SignalingStem cells internationalJanuary 1, 2023He WG, Deng YX, Ke KX, Cao XL, Liu SY, Yang YY, Luo HH, Yao XT, Gao X, Du Y, et al.phosphatase inhibitors (B14002 and B15002, Bimake, Shanghai, China); all procedures were carried out on ice. Protein G magnetic beads (S1430, NEB China group, Beijing, China) were prewashed with 30 μL lysis buffer. Cell lysates were incubated

(10) NQO1 regulates cell cycle progression at the G2/M phaseTheranosticsJanuary 1, 2023Oh ET, Kim HG, Kim CH, Lee J, Kim C, Lee JS, Cho Y, Park HJ.sodium orthovanadate, and sodium fluoride. Total cell protein (1 mg) was incubated with 25 μl washed Protein G-magnetic beads (New England Biolabs) at 4°C for 1 h. Cleared lysates were incubated overnight at 4°C with 5 μg mouse

(11) TMBIM5 is the Ca 2+ /H + antiporter of mammalian mitochondriaEMBO reportsDecember 6, 2022Shane Austin, Ronald Mekis, Sami E M Mohammed, Mariafrancesca Scalise, Wen‐An Wang, Michele Galluccio, Christina Pfeiffer, Tamara Borovec, Katja Parapatics, Dijana Vitko, et al.oxide, and osmium tetroxide were from Merck (Darmstadt, Germany), protein G magnetic beads from NEB (#S1430s), ProtA/G agarose and DMEM (#41966‐029) from Thermo Fisher Scientific (NativeMark™ #LC0725) NativePAGE™

(12) Methyltransferase like 7B is a potential therapeutic target for reversing EGFR-TKIs resistance in lung adenocarcinomaMolecular CancerDecember 1, 2022Huibin Song et al.(NEB, Hertfordshire, UK) was applied for m6A immunoprecipitation. Anti-m6A antibody was pre-bound to Protein G magnetic beads in reaction buffer for 30 min. The fragmented mRNA was incubated with m6A-antibody-bound protein G magnetic

(13) Antibody response to oral biofilm is a biomarker for acute coronary syndrome in periodontal diseaseCommunications BiologyDecember 1, 2022Mariliis Jaago, Nadežda Pupina, Annika Rähni, Arno Pihlak, Helle Sadam, Nihal Engin Vrana, Juha Sinisalo, Pirkko Pussinen, Kaia Palmparticles) and immunoglobulin G (IgG) fraction was recovered using protein G-coated magnetic beads (S1430S, NEB). Captured phage DNA was analyzed by Illumina HiSeq sequencing of 50-bp single end reads using

(14) Activity dependent dissociation of the Homer1 interactomeScientific ReportsDecember 1, 2022Mason Stillman, Jonathan D. Lautz, Richard S. Johnson, Michael J. MacCoss, Stephen E. P. Smithand normalized by the addition of lysis buffer. Lysates were pre-cleared by incubation with 25 μl of Protein G magnetic beads (NEB) for 1 h at 4 °C with rotation followed by magnetic removal of beads. 10 μg of anti-Homer1 antibody

(15) Poly ADP-ribosylation of SET8 leads to aberrant H4K20 methylation in mammalian nuclear genomeCommunications biologyNovember 25, 2022Estève PO, Sen S, Vishnu US, Ruse C, Chin HG, Pradhan S.(50 mM HEPES, pH 7.5, 250 mM NaCl, and 1% Triton X-100). The antibodies were captured using 50 µl of protein G magnetic beads (S1430, New England Biolabs) incubated for 60 min with end over end mixing at 4 °C. After 3 washes with

(16) Systematic discovery and functional dissection of enhancers needed for cancer cell fitness and proliferationCell ReportsNovember 8, 2022Poshen B. Chen, Patrick C. Fiaux, Kai Zhang, Bin Li, Naoki Kubo, Shan Jiang, Rong Hu, Emma Rooholfada, Sihan Wu, Mengchi Wang, et al.M2200S T4 PNK,NEB,Cat# M0201S Protein A Magnetic Beads,NEB,Cat# S1425S Protein G Magnetic Beads,NEB,Cat# S1430S Phusion High-Fidelity DNA Polymerase,NEB,Cat# M0530S Puromycin,InvivoGen,Cat# ant-pr-1 Blasticidin

(17) Differential patterns of cross-reactive antibody response against SARS-CoV-2 spike protein detected for chronically ill and healthy COVID-19 naÃ¯ve individualsScientific ReportsOctober 7, 2022Mariliis Jaago, Annika RÃ¤hni, NadeÅ¾da Pupina, Arno Pihlak, Helle Sadam, JÃ¼rgen Tuvikene, Annela Avarlaid, Anu Planken, Margus Planken, Liina Haring, et al.phage-immunoglobulin G (IgG) antibody complexes were recovered using protein G-coated magnetic beads (S1430S, New England Biolabs). Captured phage DNA was analysed by next generation sequencing (Illumina HiSeq,

(18) Protein interaction, cytotoxic, transcriptomic and proteomic responses to structurally distinct EPAC1 activators in HUVECsScientific ReportsOctober 5, 2022Jolanta Wiejak, Urszula Luchowska-StaÅ„ska, Pingyuan Wang, Jia Zhou, Pasquale Maffia, David Morgan, Graeme Barker, Stephen J. Yarwoodwas added to cleared lysates and incubated for 30 min at 4 °C on a rotator. Following this, 10 µl of protein G magnetic beads (New England Biolabs, Ipswich, USA) was added to each lysate containing the antibody, followed by a

(19) Epitranscriptome profiling of spleen mRNA m6A methylation reveals pathways of host responses to malaria parasite infectionFrontiers in ImmunologySeptember 20, 2022Luoluo Wang et al.with EpiMark® N6-Methyladenosine Enrichment Kit (E1610S, New England Biolabs). RNAs were eluted from protein G magnetic beads in 100 μL of Buffer RLT (79216, Qiagen) followed by precipitation. The RNAs were dissolved in 12 μL

(20) Mettl3-dependent m⁶A modification attenuates the brain stress response in DrosophilaNature communicationsSeptember 14, 2022Perlegos AE, Shields EJ, Shen H, Liu KF, Bonini NM.EpiMark N6-Methyladenosine Enrichment kit protocol with some minor alterations described. About 30 ul of protein G-magnetic beads (NEB, #S1430) were washed and resuspended in IP buffer (150 mM NaCl, 10 mM Tris-HCL, 0.1% NP-40). About

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(1)

11/20/2015Protein G Magnetic Beads M MThese are good beads to be used for immunoprecipitations. They bind a variety of IgGs in different species and are compatible with the magnetic rack to separate them from IP slurry.