Anti-C3 antibody [11H9] ab11862 from Abcam

Product Specs
ItemAnti-C3 antibody [11H9]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab11862
Antibody TypePrimary
Quantity50 UG
Clone11H9
ApplicationsELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Frozen sections), Immunoprecipitation, Western blot
HostRat
ClonalityMonoclonal
IsotypeIgG2a
Conjugate/TagUnconjugated
Format0.2 µm filtered
Concentration0.1 mg/ml
Gene NameC3
ReactivityMouse
TargetC3

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Abcam

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United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Bruton's tyrosine kinase-bearing B cells and microglia in neuromyelitis optica spectrum disorderJ NeuroinflammationDecember 21, 2023Ye Liu, Zhenning Huang, Tian-Xiang Zhang, Bin Han, Guili Yang, Dongmei Jia, Li Yang, Qiang Liu, Alexander Y. L. Lau, Friedemann Paul, et al.anti-GFAP (1:1000, Abcam, ab4648), rabbit anti-AQP4 (1:100, Millipore, ABN411), rat anti-C3 (1:200, Abcam, ab11862), rabbit anti-S100A10 (1:500, Immunoway, YT4198), rabbit anti-Phospho-Btk (Tyr344) (1:200, Thermo Fisher Scientific

(2) Group 3 Innate Lymphoid Cells Exacerbate Lupus Nephritis by Promoting B Cell Activation in Kidney Ectopic Lymphoid StructuresAdvanced ScienceNovember 1, 2023Feng Li, Zhou Liang, Haojie Zhong, Xinrong Hu, Ziwen Tang, Changjian Zhu, Jiani Shen, Xu Han, Ruoni Lin, Ruilin Zheng, et al.Then, sections were stained alone or in combination with antibodies against IgG (ab150113, Abcam), C3 (ab11862, Abcam), RORγt (14‐6988‐82, eBioscience), CD3 (ab5690, Abcam), B220 (14‐0452‐82, eBioscience), CD138

(3) Absent in melanoma 2 mediates aging‐related cognitive dysfunction by acting on complement‐dependent microglial phagocytosisAging CellJuly 1, 2023Lei Ye, Shu Shu, Junqiu Jia, Min Sun, Siyi Xu, Xinyu Bao, Huijie Bian, Yi Liu, Meijuan Zhang, Xiaolei Zhu, et al.anti‐MAP‐2 (1:1000, Abcam, ab5392); rat anti‐C1q (1:500, Abcam, ab11861); rat anti‐C3 (1:500, Abcam, ab11862). The samples were washed with PBS three times (10 min each) and incubated with secondary antibodies

(4) A role for P-selectin and complement in the pathological sequelae of germinal matrix hemorrhageJournal of NeuroinflammationJune 16, 2023Devin Hatchell, Mohammed Alshareef, Tyler Vasas, Silvia Guglietta, Davis Borucki, Chunfang Guo, Khalil Mallah, Ramin Eskandari, Stephen Tomlinsonthe proximity of the injury site. Primary antibodies used for staining were: anti-C3 (Abcam, Cat. #: ab11862, 1:200), anti-Iba1 (Invitrogen, Cat. #: PA5-21274, 1:200), and anti-CD62p (R&D, Cat. #: AF737, 1:200).

(5) Complement Drives Chronic Inflammation and Progressive Hydrocephalus in Murine Neonatal Germinal Matrix HemorrhageInternational Journal of Molecular SciencesJune 15, 2023Mohammed Alshareef, Devin Hatchell, Tyler Vasas, Khalil Mallah, Aakash Shingala, Jonathan Cutrone, Ali Alawieh, Chunfang Guo, Stephen Tomlinson, Ramin Eskandariindividual cells. Primary antibodies used for staining were anti-HO-1 (Abcam, Cambridg, UK. Cat. #: ab11862, 1:200), anti-C5b-9 (Millipore Sigma, St. Louis, MO, USA. Cat. #: 204903-1MG,1:200), anti-TER-119 (Invitrogen,

(6) Single-cell RNA sequencing reveals the fragility of male spermatogenic cells to Zika virus-induced complement activationNature CommunicationsApril 29, 2023Wei Yang, Li-Bo Liu, Feng-Liang Liu, Yan-Hua Wu, Zi-Da Zhen, Dong-Ying Fan, Zi-Yang Sheng, Zheng-Ran Song, Jia-Tong Chang, Yong-Tang Zheng, et al.overnight at 4 °C, including rat anti-mouse C1q (1:100, Abcam, ab11861), rat anti-mouse C3 (1:100, Abcam, ab11862), rabbit anti-mouse MAC (1:100, Abcam, ab55811), rabbit anti-mouse CD45 (1:100, Abcam, ab10558), rabbit

(7) A Role for P-selectin and Complement in the Pathological Sequelae of Germinal Matrix HemorrhageResearch SquareMarch 1, 2023Devin Hatchell, Mohammed Alshareef, Tyler Vasas, Silvia Guglietta, Davis Borucki, Chunfang Guo, Khalil Mallah, Ramin Eskandari, Stephen Tomlinsonthe proximity of the injury site. Primary antibodies used for staining were: anti-C3 (Abcam, Cat. #: ab11862, 1:200), anti-Iba1 (Invitrogen, Cat. #: PA5–21274, 1:200), and anti-CD62p (R&D, Cat. #: AF737, 1:200).

(8) A porcine islet-encapsulation device that enables long-term discordant xenotransplantation in immunocompetent diabetic miceCell Reports MethodsJanuary 23, 2023Kumiko Ajima, Naoto Tsuda, Tadashi Takaki, Shoji Furusako, Shigeki Matsumoto, Koya Shinohara, Yzumi Yamashita, Sayaka Amano, Chinatsu Oyama, Masayuki Shimodaas primary antibody at a dilution of 1:200. Rat monoclonal anti-C3 antibody was obtained from Abcam (ab11862) and used as primary antibody at a dilution of 1:100. Goat polyclonal anti-guinea pig IgG (H + L) secondary

(9) CRISPRi screens in human iPSC-derived astrocytes elucidate regulators of distinct inflammatory reactive statesNature neuroscienceNovember 1, 2022Leng K, Rose IVL, Kim H, Xia W, Romero-Fernandez W, Rooney B, Koontz M, Li E, Ao Y, Wang S, et al.antibodies against ISG15 (rabbit, clone: 1H9L21, Invitrogen 703132, 1:100), C3 (rat, clone: 11H9, Abcam ab11862, 1:50), and GFAP (chicken, polyclonal, Aves Labs GFAP, 1:500). Sections were washed three times with

(10) The alarmin interleukin-1α triggers secondary degeneration through reactive astrocytes and endothelium after spinal cord injuryNature communicationsOctober 2, 2022Bretheau F, Castellanos-Molina A, Bélanger D, Kusik M, Mailhot B, Boisvert A, Vallières N, Lessard M, Gunzer M, Liu X, et al.rat anti-BrdU (1:1000, clone BU1/75 [ICR1], Abcam, ab6326), rat anti-C3 (1:100, clone 11H9, Abcam, ab11862), mouse anti-CC1 (1:1000, clone CC1, Abcam, ab16794), rat anti-CD11b (1:250, clone 5C6, AbD Serotec,

(11) Crry silencing alleviates Alzheimer's disease injury by regulating neuroinflammatory cytokines and the complement systemNeural regeneration researchAugust 1, 2022Zhu XC, Liu L, Dai WZ, Ma T.monoclonal,sc-81603,"Santa Cruz Biotechnology, Santa Cruz, CA, USA" C3,1:500,AB_2066623,Rat monoclonal,ab11862,"Abcam, Cambridge,UK" C3b,1:2000,AB_627277,Mouse monoclonal,sc-28294,"Santa Cruz Biotechnology, Santa

(12) Effect of rottlerin on astrocyte phenotype polarization after trimethyltin insult in the dentate gyrus of miceJournal of neuroinflammationJune 11, 2022Hwang Y, Kim HC, Shin EJ.protein (GFAP, 1:500; #MAB3402, Millipore, Temecula, CA, USA), complement component 3 (C3, 1:100; #ab11862, Abcam, Cambridge, MA, USA), S100A10 (1:200; #AF2377, R&D Systems, Minneapolis, MN, USA), Iba-1 (1:500,

(13) Melanoma-Secreted Amyloid Beta Suppresses Neuroinflammation and Promotes Brain MetastasisCancer discoveryMay 2, 2022Kleffman K, Levinson G, Rose IVL, Blumenberg LM, Shadaloey SAA, Dhabaria A, Wong E, Galán-Echevarría F, Karz A, Argibay D, et al.Rabbit Anti-Iba1 (Wako Chemicals 019–19741, RRID:AB_839504) 1:500 – 48 hours, Rat Anti-C3 [11H9] (Abcam ab11862, RRID:AB_2066623) 1:50 – 72 hours, Rabbit Anti-TMEM119 (Abcam ab209064, RRID:AB_2800343) 1:100 – 72

(14) Trastuzumab/pertuzumab combination therapy stimulates antitumor responses through complement-dependent cytotoxicity and phagocytosisJCI insightMarch 22, 2022Tsao LC, Crosby EJ, Trotter TN, Wei J, Wang T, Yang X, Summers AN, Lei G, Rabiola CA, Chodosh LA, et al.prepared as described above and stained with 2 μg/mL of rat anti–mouse C3 antibody (clone 11H9, Abcam, ab11862) and 2 μg/mL rabbit anti–mouse C5 antibody (Abcam, ab11898) in 1% BSA/PBS at 4°C for 30 minutes. Cells

(15) A Role of Complement in the Pathogenic Sequelae of Mouse Neonatal Germinal Matrix HemorrhageInternational Journal of Molecular SciencesMarch 9, 2022Mohammed Alshareef, Khalil Mallah, Tyler Vasas, Ali Alawieh, Davis Borucki, Christine Couch, Jonathan Cutrone, Chelsea Shope, Ramin Eskandari, Stephen Tomlinsonthe field. The primary antibodies used for staining were: anti-C3 (Abcam, Waltham, MA, USA, Cat. #: ab11862, 1:200), anti-NeuN (Abcam, Waltham, MA, USA, Cat. #: ab104225, 1:200), anti-Iba1 (Invitrogen, Waltham,

(16) Mannose-binding lectin and complement mediate follicular localization and enhanced immunogenicity of diverse protein nanoparticle immunogensCell reportsJanuary 11, 2022Read BJ, Won L, Kraft JC, Sappington I, Aung A, Wu S, Bals J, Chen C, Lee KK, Lingwood D, et al.AB_2562979 "αCD38, clone 90, in AF488",BioLegend,Cat#102714; RRID: AB_528796 "αC3, clone 11H9",Abcam,Cat#ab11862; RRID: AB_1119819 "α-sialic acid, polyclonal",LSBio,Cat#LS-C664155 "Chemicals, peptides, and recombinant

(17) Divergent complement system activation in two clinically distinct murine models of multiple sclerosisFrontiers in immunologyJanuary 1, 2022Linzey M, DiSano K, Welsh N, Pachner A, Gilli F.blocking, spinal cord sections were incubated with: C1q (1:100, Abcam #Ab11861), C3 (1:100, Abcam #Ab11862), C5b-C9 (1:50, EMD Millipore #204903-1MG), IBA1 (1:500, Fujifilm Wako Pure Chemical Co. #019-19741),

(18) Complement C5 is not critical for the formation of sub-RPE deposits in Efemp1 mutant miceScientific ReportsDecember 1, 2021Donita L. Garland, Eric A. Pierce, Rosario Fernandez-Godinosections were blocked with 1% BSA and incubated with primary antibodies anti C3/C3b/iC3b/C3d/C3dg (AB11862, Abcam), EFEMP1 (SDIX), MAC (C5b-9 AB55811, Abcam), TIMP-3 (AB39184 Abcam), ELN (sc17581, Santa Cruz Biotechnology

(19) Iron chelation suppresses secondary bleeding after intracerebral hemorrhage in angiotensin II‐infused miceCNS Neuroscience & TherapeuticsNovember 1, 2021Jie Wang, Xiao‐qin Tang, Min Xia, Cheng‐cheng Li, Chao Guo, Hong‐fei Ge, Yi Yin, Bo Wang, Wei‐xiang Chen, Hua FengWako, 019‐19741), rat anti‐CD68 (diluted 1:100, Abcam, ab53444), rat anti‐C3 (diluted 1:100, Abcam, ab11862), mouse anti‐AQP4 (diluted 1:100, Abcam, ab9512), rabbit anti‐GFAP (diluted 1:500, Abcam, ab7260), and

(20) Lung mesenchymal stromal cells influenced by Th2 cytokines mobilize neutrophils and facilitate metastasis by producing complement C3Nature communicationsOctober 27, 2021Zheng Z, Li YN, Jia S, Zhu M, Cao L, Tao M, Jiang J, Zhan S, Chen Y, Gao PJ, et al.(Leica Biosystems). Primary antibodies (1 : 200) were applied as follows: Rat anti-mouse C3 (Abcam ab11862), Rabbit anti-C3 (Abcam ab200999), Rat anti-mouse Nestin (Abcam ab81462), Rat anti-mouse Ly6G (Abcam

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Anti-C3 antibody [11H9] from Abcam

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Anti-C3 antibody [11H9] from Abcam

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