Fig 2: Effects of FIGNL1 protein expression level on patient survival. (A) FIGNL1 protein expression level in cancerous and para-carcinoma tissues using immunohistochemical staining. A higher number of cancerous tissues showed positive FIGNL1 staining. (B) Survival curves of patients with lung cancer and low or high protein expression level of FIGNL1. FIGNL1, fidgetin-like 1.

Fig 3: Effect of FIGNL1 knockdown on in vivo tumor formation. BALB/c nude mice were divided into two groups: One group were injected with shCtrl H1299 cells, while the other group were injected with shFIGNL1-2 H1299 cells. The statistical graph indicated that compared with that in the mice injected with shCtrl, FIGNL1 knockdown significantly reduced the formation of the tumor from day 13. ***P<0.001, ****P<0.0001. n=7. sh, short hairpin; Ctrl, control; FIGNL1, fidgetin-like 1.

Fig 4: Representative immunohistochemical images showing FIGNL1 expression in cancerous and para-carcinoma tissues. (A) FIGNL1 negative staining in para-carcinoma tissues (score, 0). (B) Negative FIGNL1 staining in carcinoma tissues (score, 0). (C) Weak positive staining for FIGNL1 in cancerous tissues (score, 1). (D) Moderate positive staining for FIGNL1 in cancerous tissues (score, 2). (E) Strong positive staining for FIGNL1 in cancerous tissues (score, 3). FIGNL1, fidgetin-like 1. SP, streptavidin-peroxidase.

Fig 5: Examination of FIGNL1 protein and mRNA expression level and its effects on cell growth rate. (A) shFIGNL1-2 decreased the mRNA expression level the most, among the three shRNAs in H1299 cell line. (B) shFIGNL1-1 led to a significant decrease in mRNA expression level in A549 cells. (C) The three shRNAs inhibited growth at different levels; shFIGNL1-2 had the strongest ability to suppress cell growth in H1299 cell line. (D) Compared with that in the shCtrl group, shFIGNL1-1 could significantly reduce cell growth in the A549 cell line. (E) FIGNL1 protein expression levels following knockdown and overexpression in the H1299 and A549 cell lines. (F) FIGNL1 mRNA expression level was significantly increased following transfection with the overexpres-sion vector in both cell lines. (G) Cell growth was significantly increased following overexpression of FIGNL1. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001. n=3. FIGNL1 knockdown using shFIGNL1-2 in H1299 cells and shFIGNL1-1 in A549 cells. sh, short hairpin; Ctrl, control; OD, optical density; NC, negative control; OE, overexpression; FIGNL1, fidgetin-like 1.