Fig 2: Overexpression of PHF19 promoted CRC cell migration and invasion by inducing EMT in vitro.Overexpression of PHF19 promoted CRC cell migration and invasion by inducing EMT in vitro. Transwell was used to examine cell migration and invasion in SW480 and HT-29 transfected with PHF19 overexpression plasmid (A–C). Wound healing assay was used to examine cell migration in SW480 and HT-29 transfected with PHF19 overexpression plasmid (D–F). Western blot was used to detect the expression of EMT related proteins (G). (***means P < 0.001).

Fig 3: PHF19 mRNA expression levels between tumor and normal tissues in CRC patients in GEO database series and TCGA-COADREAD database.PHF19 mRNA expression levels between tumor and normal tissues in CRC patients in GEO database series including GSE21510 (A), GSE21815 (B), GSE9348 (C), GSE18105 (D), GSE8671 (E), GSE44076 (F), GSE10715 (G), and the TCGA-COADREAD database (H).

Fig 4: High PHF19 expression positively correlates with Cell Cycle in CRC patients.High PHF19 expression positively correlates with Cell Cycle in CRC patients. Top ten signaling pathway in CRC tissue based on GSEA (A). Enrichment of KEGG pathways by GSEA using the TCGA database (B). The enrichment plot shows that GSEA analysis of genes involved in cell cycle (C, D, E).

Fig 5: Co-expression analysis of PHF19 using LinkOmics.Co-expression analysis of PHF19 using LinkOmics. Highly correlated genes were identified by Pearson test in COADREAD cohort (A). Heat maps showing top 50 genes correlated with PHF19 in COADREAD. Red indicates positive scores and blue indicates negative scores (B, C). Enrichment of GO annotations and KEGG pathways of PHF19 in the COADREAD cohort (D, E).