Fig 1: SG biogenesis is impaired Magel2p?/m+ mice, human iN with PWS deletion, and DPSC-derived neurons in PWS patients.(A and B) Representative images (A) and quantitation (B) of SG (yellow arrows) by electron microscopy of 8-week-old Magel2+/+ and Magel2p?/m+ + mouse medial eminence region of hypothalamus, n = 2 per genotype; >30 images were analyzed. Data shown as mean ± SD and analyzed by unpaired, 2-tailed t test; *P < 0.05. (C and D) Representative images (C) and quantitation (D) of immunofluorescence staining with CHGB and CHGA in control and PWS iN. Each data point represents 1 induction experiment (n = 5), plotted as mean ± SD and analyzed by 1-way ANOVA. (E and F) Representative images (E) and quantitation (F) of immunofluorescence staining with PCSK2 and DAPI in control and PWS deletion DPSC-derived neurons. Each data point represents 1 individual, plotted as mean ± SD and analyzed by unpaired, 2-tailed t test. *P < 0.05, ***P < 0.001, and ****P < 0.001.