Fig 1: LINC00458 is necessary for soft substrate–induced endoderm commitment.(A) hPSCs were cultured on TCPS and transfected with LNA GapmeRs targeting LINC00458 to determine knockdown efficiency. (B) hPSCs were cultured on soft substrate with LNA GapmeRs targeting LINC00458, and the expression of endodermal genes SOX17, FOXA2, EOMES, and GATA6 was analyzed by qRT-PCR. (C) Immunoblotting analysis of the FOXA2 and SOX17 expressions in hPSCs cultured on soft substrates with LNA GapmeRs targeting LINC00458. (D and E) Immunofluorescence detection of FOXA2 and SOX17 expressions in hPSCs cultured on hard substrates with LINC00458 transfection. The results are presented as means ± SD of triplicates. **P < 0.005, *P < 0.05, two-sided Student’s t test.