Fig 1: Ndufs4 deficiency shows altered synaptic protein expression without neuronal loss. (A) Representative image of co-immunofluorescence staining of NeuN and Caspase 3 in hippocampus and olfactory bulb (OB) of WT mouse and Ndufs4-KO. Insets enlarge the boxed area. Low magnification green indicates NeuN and red indicates caspase 3, scale bar: 400 µm. In high magnification red indicates caspase 3 with no green stain, scale: 20 µm. (B) Representative images of Western blot for synaptophysin, PSD95, Vglut1, EAAT1, EAAT2, EAAT3 in WT and Ndufs4-KO mouse hippocampus. (C–H) Analysis of band densities by ImageJ (n = 3).