Fig 2: ANGPT2 knockdown improved the inflammatory response, barrier dysfunction and ER stress of LPS-induced IEC-6 cells via suppressing Notch signaling pathway. (a–d) The transfected or untransfected IEC-6 cells were induced with LPS (10 µg/ml) for 24 h. Jagged-1 (JAG), an agonist of Notch, was used for treatment. The levels of PGE2, TNF-a, IL-1ß and IL-6 were checked by ELISA. (e) The cell monolayer permeability was detected using TEER. (f) The expressions of ZO-1, occludin and claudin-1 were measured by western blot. (g) The expressions of GRP79, CHOP, p-PERK and PERK were measured by western blot. *p < 0.05, **p < 0.01, ***p < 0.001