Fig 1: Conditional deletion of Eya1 leads to a reduction in the numbers of Pou2f3+, Six1+ and IP3R3+ taste cells. (A–E) Immunostaining for Pou2f3 (A), Six1 (B), or co-immunostaining for Six1/Krt8 (C), IP3R3 (D) or co-immunostaining for IP3R3/Ncam (E) in wild-type control and Eya1cKO taste buds at ∼6–8 weeks of age. Sections were also counterstained with diluted hematoxylin, and bright-field images were taken to outline the taste buds. Scale bars: 50 μm (A–C) and 20 μm (D, E).
Fig 2: Eya2 is expressed in the CVP and FOP taste buds, and Eya2-deficient mice show a reduced number of Type II taste cells. (A, B) X-Gal staining on sections showing broad expression of Eya2 LacZ in the CVP and FOP trench epithelium at P14 (A) and the adult CVP trench epithelium (B). (C) Higher magnification showing β-Gal+ cells in some basal progenitors (arrows) and cells surrounding the taste pore (red asterisks) and inside the taste buds. Some sections were counterstained with diluted hematoxylin (A and two lower panels in (C). (D–F) Immunostaining for Pou2f3 (D), Six1(E), and co-immunostaining for IP3R3/Ncam (F) in wild-type control and Eya2-deficient adult taste buds. Scale bars: 50 μm (A, B) and 20 μm (C–E).
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