Fig 1: Flow cytometry and immunofluorescence of Sca1+ FAPs and ITGA7+ MuSCs.(A) Scatter plots of Sca1+ FAPs (from 45-d old wt mice) stained with antibodies raised against CD140a-APC (PDGFRa) (left panel). Scatter plots of ITGA7+ MuSCs (from 45-d-old wt mice) stained with antibodies raised against ITG7-APC and Pax7-FITC (right panel). (B) Percentage of Sca1+ FAPs expressing CD140a (PDGFRa) (left table). Percentage of ITGA7+ MuSCs co-expressing ITGA7 and Pax7 (left table). Data from three independent cell preparations are shown. (C) Representative immunofluorescence showing the expression of CD140a (PDGFRa) in FAPs and the expression of Pax7 and MyoD in MuSCs.